Contributors: Gabriel Fiorin, Andrea Sanchez-Vallet, Daniela Thomazella, Paula Prado, Leandro Nascimento, Antonio Figueira, Bart Thomma, Goncalo Pereira, Paulo Jose Teixeira
... Source data of the paper "Suppression of plant immunity by fungal chitinase-like effectors".
Contributors: Richard Zimmermann, Regine Stutz
... In mammalian cells, one-third of all polypeptides are transported into/across the ER-membrane via the Sec61-channel. While the Sec61-complex facilitates translocation of all polypeptides with amino-terminal signal peptides (sp) or transmembrane helices, the Sec61-associated TRAP-complex supports translocation of only a subset, i.e. in a substrate-specific manner. To characterize TRAP-dependent precursors, we combined siRNA-mediated TRAP depletion in human cells, label-free quantitative proteomics, and differential expression analysis. The results were validated by western blotting and quantitative RT-PCR as well as under conditions of over-production of TRAP-dependent precursors and their mutated variants in the presence or absence of MG132, a proteasome inhibitor. Sp analysis of TRAP-clients revealed above-average glycine-plus-proline content as the distinguishing feature. Thus, TRAP acts as an sp-receptor on the ER-membrane’s cytosolic face for precursor polypeptides with high glycine-plus-proline content in their sp and triggers substrate-specific (and regulated) opening of the Sec61-channel by its interaction with the ER-lumenal hinge of Sec61α. The work is about to be published under the title Proteomics reveals signal peptide features determining the client specificity in human TRAP-dependent ER protein import by Duy Nguyen, Regine Stutz, Stefan Schorr, Sven Lang, Stefan Pfeffer, Hudson H. Freeze, Friedrich Förster, Volkhard Helms1 Johanna Dudek & Richard Zimmermann Here we present the original western blot data plus additional SP and TMH analyses, which are presented in a pptx figure and a xlsx table, respectively . Additional physicochemical properties of TRAP clients with SP or TMH. In addition to the hydrophobicity score and glycine/proline (GP) content, we determined the ΔGapp values of SP sequences (a) and transmembrane helices (TMH) (b) with the ΔGapp predictor for TM helix insertion (http://dgpred.cbr.su.se) and plotted these values against the relative count (Density). Glycine and glycine plus proline content of human signal peptides. We used custom scripts to compute the glycine (G) and glycine plus proline (GP) content of human SP sequences from UniProtKB entries. GP content was calculated as the total fraction of glycine and proline in the respective sequence. Notably, 1382 SP of a total of 3513 have a GP content of > 15%, the proposed threshold for TRAP involvement and, therefore, potential regulation by phosphorylation and/or calcium binding by TRAP subunit α.
an approach to improve the mechanical properties of LM24 alloy via dislocation enhanced precipitates
Contributors: yijie zhang
... The main idea is to develop an approach to improve the mechanical properties of LM24 alloy by the enhanced the precipitates strengthening when carrying on the directly ageing treatment. To achieve this, the formation of precipitates is promoted by the dislocation movement and to optimize the size of the precipitates by accelerating the diffusion along dislocation.
Contributors: Alessandro Borgognone
... Background, surgery and perspectives of the oncoplastic breast surgery are examined and discussed .
Contributors: Hsiang-Chen Chui, Wei-Chen Lin, Yi-Hsi Chen, Jowtsong Shy, Hong-Zui Chen
... The Supplement information of this research work. It included the optical layout, the spectra analysis under different temperatures, and applied currents.
Contributors: Richard Zimmermann, Marie-Christine Klein
... The endoplasmic reticulum (ER) requires a constant ATP supply. However, the carrier that catalyzes ATP uptake into the ER was unknown. In search of the elusive ATP/ADP carrier we screened gene expression datasets for member(s) of the family of solute carriers that are co-expressed with BiP, the major ER resident ATP consumer, and are ER membrane proteins. We identified SLC35B1 as a potential candidate. Heterologous expression of SLC35B1 in E. coli revealed that SLC35B1 is highly specific for ATP and ADP and acts in antiport mode. Moreover, siRNA mediated depletion of SLC35B1 from HeLa cells reduced ER ATP levels and BiP activity. Thus, human SLC35B1 provides ATP to the ER and was named AXER (ATP/ADP exchanger in the ER membrane). Furthermore, we began to unravel an ER to cytosol low energy response regulatory axis (termed lowER) that is central for maintaining ER ATP supply under steady state and stress conditions. This work is about to be published under the title AXER is an ATP/ADP exchanger in the membrane of the endoplasmic reticulum by Marie-Christine Klein, Katharina Zimmermann, Stefan Schorr, Martina Landini, Patrick A. W. Klemens, Jacqueline Altensell, Martin Jung, Elmar Krause, Duy Nguyen, Volkhard Helms, Jens Rettig, Claudia Fecher-Trost, Adolfo Cavalié, Markus Hoth, Ivan Bogeski, H. Ekkehard Neuhaus, Richard Zimmermann, Sven Lang and Ilka Haferkamp Here we present the original western blot data.
Contributors: Xuping Xie
... As shown in Fig. 3h, the C7a/t virus contained two C variants: HA-C (~12.5 kDa) and C7 (~10.5 kDa) proteins with an estimated molecular ratio of 1:30, whereas the WT ZIKV had only full-length C protein (~11.4 kDa). As expected, both C7a/t and WT viruses contained mature M and E proteins. As controls, culture medium from replicon-transfected cells contained only NS1 protein, with no detectable structural proteins (C, M, or E). The data suggest that C7a/t ZIKV contains both HA-C and C7 proteins. Western blot analysis of C7a/t virus. Supernatants from replicon RNA-transfected BHK-HA-C cells (day 4 p.t.), WT RNA-transfected Vero cells (day 4 p.t.), and C7a RNA-transfected BHK-HA-C cells (day 4 p.t.) were analyzed for viral proteins (HA-C, C, M, E, and NS1) using Western blotting.
Contributors: Phil Prangnell
... Automated image analysis measurements and final selected images for paper
Contributors: Santiago Emilio Díaz Azpíroz
... These data speak about my concept of architecture, the relationships instead of people through public space to show their idiosyncracy or identity as a community
Supplementary material (Development of a customized eddy current system for high-speed inspection of unidirectional carbon fiber reinforced polymer)
Contributors: Miguel Machado
... EC density, EC vectors and secondary magnetic field for different ECT probes operation modes