Asymmetric centromere and dynamic microtubules coordinate to ensure biased sister chromatid segregation in Drosophila male germline stem cells
Contributors: Rajesh Ranjan
... Many stem cells utilize asymmetric cell division (ACD) to produce a self-renewed stem cell and a differentiating daughter cell. How non-genic information could be inherited differentially to establish distinct cell fates is not well understood. Here, we report a series of spatiotemporally regulated asymmetric components, which ensure biased sister chromatid attachment and segregation during ACD of Drosophila male germline stem cells (GSCs). First, sister centromeres are differentially enriched with proteins involved in centromere specification and kinetochore function. Second, temporally asymmetric microtubule activities and polarized nuclear envelope breakdown allow for the preferential recognition and attachment of microtubules to asymmetric sister kinetochores and sister centromeres. Abolishment of either the asymmetric sister centromeres or the asymmetric microtubule activities results in randomized sister chromatid segregation. Together, these results provide the cellular basis for partitioning epigenetically distinct sister chromatids during stem cell ACDs, which opens new directions to study these mechanisms in other biological contexts.
Data/Software for "Presynaptic Mitochondria Volumes and Complexity of Subsynaptic Distribution Increase During Development at a High-fidelity Synapse"
Contributors: Connon I. Thomas, Christian Keine, Satoko Okayama, Rachel Satterfield, Morgan Musgrove, Debbie Guerrero-Given, Naomi Kamasawa, Samuel M. Young, Jr.
... Contains data and software from the publication: "Presynaptic Mitochondria Volumes and Complexity of Subsynaptic Distribution Increase During Development at a High-fidelity Synapse" currently under review. The preprint to this data set has been published on bioRxiv (https://doi.org/10.1101/689653). In this study, we created a helper-dependent adenoviral vector (HdAd) to co-express cytoplasmic EGFP and a genetically encoded peroxidase marker (mito-APEX2) at the calyx of Held, an excellent model for deciphering regulatory mechanisms of presynaptic function. ABSTRACT: The calyx of Held, a large glutamatergic presynaptic terminal in the auditory brainstem undergoes developmental changes to support the high action-potential firing rates required for auditory information encoding. In addition, calyx terminals are morphologically diverse which impacts vesicle release properties and synaptic plasticity. Mitochondria influence synaptic plasticity through calcium buffering and are crucial for providing the energy required for synaptic transmission. Therefore, it has been postulated that mitochondrial levels increase during development and contribute to the morphological-functional diversity in the mature calyx. However, the developmental profile of mitochondrial volumes and subsynaptic distribution at the calyx of Held remains unclear. To provide insight on this, we developed a helper-dependent adenoviral vector (HdAd) that expresses the genetically encoded peroxidase marker for mitochondria, mito-APEX, at the mouse calyx of Held. We developed protocols to detect labeled mitochondria for use serial block face SEM (SBF-SEM) to carry out semi-automated segmentation of mitochondria, high-throughput whole terminal reconstruction and presynaptic ultrastructure in mice of either sex. Subsequently, we measured mitochondrial volumes and subsynaptic distributions at the immature postnatal day 7 (P7) and the mature (P21) calyx. We found an increase of mitochondria volumes in terminals and axons from P7 to P21 but did not observe differences between stalk and swelling subcompartments in the mature calyx. Based on these findings, we propose that mitochondrial volumes developmentally increase to support high firing rates but have limited contribution to morphological-functional diversity at the calyx. Data are sorted by the figures they appear in. Media (movies and 3D models) and custom-written software are located in separate folders.
Contributors: Nathaniel Hathaway
... Raw data from Headley et al. 2019.
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Evaluation of a virtual basic dermatology curriculum for dermoscopy using the Triage Amalgamated Dermoscopic Algorithm (TADA) for novice dermoscopists.
Contributors: Jason Susong
... Supplemental Diagrams and Video.
Contributors: UnaElsLive Natra, mdgmatest5 live
... RDM - File Type Support 21May2019 ElsCustomer Apart from .u3d all files preview [ .obj / .ply / .vtk / .stl / .ent / .brk / .pdb / .pse / .mol / .mol2 / .cif / .u3d / .dcm / .nii] - .pse is not supported
Contributors: Carlos Palacio
... Raw data for the internal Project 2016210 "Preparation and characterization of magnetic nanoparticles based in ferrites"--> UAN-UdeA.
Contributors: Ravindra verma, PRAKASH BISEN
... To remain competitive and survive in the developing economy, farmer has to adopt the scientific and technological approach for farming. Integrated farming with crop diversification is a sustainable agricultural development concept towards accumulation and utilization of cultivable land. Cultivating suitable multiple crop like short term vegetable, long term tree plantation, herbal farming are in order to generate higher farm income. Increasing awareness about physical, mental wellness and inimical effects of modern medicines peoples again diversify for the use of herbal medicines. Our exercise demonstrates the integrated path for cultivation of Chlorophytum Borivilianum (Safed Musali) with other high valued medicinal herbs like Momordica Charantia L. (Bitter gourd), Mucuna Pruriens L. (Velvet Beans), and Jatropha Curcas in the cultivated land of Gwalior in India. It has been noticed that all the four herbs are eco-friendly with each other and can cultivate on the same land. Another herb Gloriosa Superba Linn (Superb Lily) is proposed for empirical purpose on the same cultivation field with Jatropha curcas. The primary objective of our study is to optimize the economy of the farm land and yield of interdependent plants. Our accomplishment and practice will help in terms of understanding integrated goal for multicropping of herbs to the economy of farmer. This study will provide the ground and contribute in future for the study of integrated herbal farming.
Data for: Modal analysis of two different types of fixed implant-supported prostheses embedded in edentulous maxillae
Contributors: Akihiro Suzuki, Shohei KASUGAI, Takafumi Otomaru, Hisashi Taniguchi, Shinji Kuroda, Hidemi Nakata, Taiji Hoshiai, Meiko Oki
... Supplementary Video 1: The modal shapes at the natural frequency of 674 Hz in Subject A. To facilitate understanding, we have provided a Supplementary video (Supplementary Video 1). Supplementary Video 2: The modal shapes at the natural frequency of 660 Hz in Subject J. To facilitate understanding, we have provided a Supplementary video (Supplementary Video 2). The maximum displacements: The maximum displacements of every measurement point. The damping ratios: The damping ratios at the natural frequency of all Subjects. Modal shapes: The modal shapes at the natural frequency of all Subjects. A repeated vibration was divided into 24 moments. The moments, including acceleration vectors showing antiphase, were extracted (From the upper left side). The number of vectors in antiphase: A repeated vibration was divided into 24 moments. The acceleration vectors showing antiphase were counted. The number of vectors in antiphase was compared.
Data for: Strain distribution of the anterolateral ligament during internal rotation at different knee flexion angles: A biomechanical study on human cadavers
Contributors: Jun Ouyang, Yibei Wang, Shiqi Li, Daorong Xu, Lei Qian, Chunyu Jiang, Peidong Sun, Maoqing Fu
... The datas are overall strain and sub-regional strain of the anterolateral ligament induced by tibial internal rotation at different knee flexion angles. The video is a dynamic display of strain distribution of the anterolateral ligament duiring internal rotation at 90° of knee flexion.
Contributors: Cian Desmond, Florent Thiebaut, Jimmy Murphy
... An aluminium cross-section approximately 1 m in length was held between 2 flat plates connected to a movable carriage in a wave basin. Under still water conditions, the cross section was moved through the wave basin. Data were collected from an array of load cells in 6 DoF. This process was repeated for four cross sections, each at 4 angles of attack and 7 velocities. These data can be used for all research scientists and engineers working in the fields of fluid mechanics and hydraulics. The data may be used specifically for the validation of numerical models