Contributors: Frederik Holdt
... This was a retrospective review to ascertain the plain film fracture detection rate of non-radiologist trauma clinicians when interpreting Lodox bodygram images in adult trauma patients. Customised searches of the Picture Archiving and Communications Systems (PACS) of two tertiary academic hospitals in the Western Cape, South Africa, were conducted to retrieve all adult trauma patients (aged 18+ years) who had Lodox bodygram scans as part of their initial workup on presentation to either of the two Level 1 Trauma Units over two consecutive months. Information collected included age, sex, mechanism of trauma, location of all fractures or dislocations visible on the Lodox bodygram and whether the patient was intubated or not. The bodygram images (which are not routinely reported by radiologists) were retrospectively reviewed by two radiologists, who noted the locations of all fractures visible on Lodox (gold standard). The follow-up imaging sequence on the PACS system was then analysed to see which of the fractures visible on Lodox received dedicated follow-up imaging (radiographs/CT/MR) since the single projection Lodox bodygram is insufficient for full characterization of the injury. Fractures seen on Lodox but without additional dedicated imaging were deemed as missed. Cases which were unclear or where follow-up imaging was delayed were clarified by review of the clinical notes. Generalised Fischer Exact Test assessed any association between the fracture site and failure of detection. An orthopaedic surgeon was consulted to ascertain which of the missed fractures needed surgical treatment. Data included are: Excel Spreadsheet: Complete Dataset Complete dataset for our study in table form. Columns are self-explanatory with totals tallied below. Excel Spreadsheet: Statistical results The results of the Generalised Fischer Exact Test used to assess any association between the fracture site and failure of detection Figure 2: Selected examples of subtle missed fractures, description included in accompanying Word document of the same name.
Contributors: Jens Kalchschmidt
... These are the raw data files of the 2nd allele targeting experiments, the Med14 SMASh and PolII alpha-amanitin western blots published in El Khattabi et al., Cell, 2019
Contributors: Joshua Soderholm
... The following collection is used to demonstrate the HailPixel survey technique as part of an AGU GRL publication. The following is an abstract for this paper: A new technique, named "HailPixel," is introduced for measuring the maximum dimension and intermediate dimension of hailstones from aerial imagery. The photogrammetry procedure applies a convolutional neural network for robust detection of hailstones against complex backgrounds and an edge detection method for measuring the shape of identified hailstones. This semi-automated technique is capable of measuring many thousands of hailstones within a single survey, which is several orders of magnitude larger than population sizes from existing sensors (e.g., a hail pad). Comparison with a co-located hail pad for an Argentinan hailstorm event demonstrates the larger population size of the HailPixel survey significantly improves the shape and tails of the observed hail size distribution. When hailfall is sparse, such as during large and giant hail events, the large survey area of this technique is especially advantageous for resolving the hail size distribution. The dataset contains the DEM and orthomosaic imagery, processing reports, final location of hail centroids, final measurements of hail major and minor axis, subset offsets and hail pad data. For more information applying the subset offsets to calculate the true position of the hail centroids please see the paper.
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Data for: A geomorphology based reconstruction of ice volume distribution at the Last Glacial Maximum across the Southern Alps of New Zealand
Contributors: William James, Duncan Quincey, Jonathan Carrivick, Neil Glasser
... Modeled LGM ice thickness for the New Zealand Southern Alps
Generation of Human PSC-Derived Kidney Organoids with Patterned Nephron Segments and a De Novo Vascular Network
Contributors: Jian Hui Low, Pin Li, Elaine Chew, Bingrui Zhou, Tian Zhang, Michelle Lian, Meng Liu, Jia Nee Foo, Juan Carlos Izpisua Belmonte, Yun Xia
... Human pluripotent stem cells-derived kidney organoids recapitulate developmental processes and tissue architectures, but intrinsic limitations, such as a lack of vasculature and functionality, have greatly hampered their application. Here we establish a versatile protocol for generating vascularized three-dimensional kidney organoids. We employ dynamic modulation of WNT signaling to control the relative proportion of proximal versus distal nephron segments, thereby producing a correlative level of VEGFA to define a resident vascular network. Single cell RNA-sequencing identifies a subset of nephron progenitor cells as a potential source of renal vasculature. These kidney organoids undergo further structural and functional maturation upon implantation. Using this kidney organoid platform, we establish an in vitro model of autosomal recessive polycystic kidney disease, the cystic phenotype of which can be effectively prevented by gene correction or drug treatment. Our studies provide new avenues for studying human kidney development, modeling disease pathogenesis, and performing patient-specific drug validation.
Astragalus granule ameliorates renal injury in diabetic rats and patients with diabetic kidney disease
Contributors: Teng Chen
... 1 Astragalus granule with losartan alleviated STZ-induced renal injury in DKD rats Significantly reduced kidney/body weight ratios and BUN were observed in AG+losartan group and Losartan group compared to Model group (P<0.01)(Figure 1). Compared to the losartan group, the ACR were also significantly reduced in AG+losartan group (P<0.05)(Figure 2). The results of histopathological tests of the renal tissues at the end of the experiment are shown in Figures 3. Pathological renal lesion significantly worsened in the model group. AG+losartan and losartan relieved glomerular hypertrophy and mesangial expansion (Figure 3). 2 In comparison with controls, the expression of TLR4, MyD88 and p-NF-κB significantly increased in the model group (P<0.05). After 12 weeks of treatment with Astragalus granules and losartan, the expression of TLR4, MyD88 and p-NF-κB protein decreased in the AG+losartan group and Losartan group (P<0.05). Compared to the losartan group, TLR4, MyD88 and p-NF-κB were also significantly reduced in AG+losartan group. In the all groups the level of NF-κB was not statistically different (Figure 4). 3 Astragalus granulesinhibited STZ-induced TNF-α and IL-1β generation in DKD rats Renal inflammation plays a key role in promoting the occurrence and development of DKD. To explore whether Astragalus granules could affect STZ-induced inflammatory cytokines production, ELISA was performed to detect the levels of TNF-α and IL-1β in blood of DKD rats. An apparent upregulation was observed in the levels of TNF-α and IL-1β in blood of DKD rats, while astragalus granules and losartan treatment strikingly inhibited the increase of TNF-α and IL-1β in DKD rats (Figure 5). 4 Astragalus granules alleviated renal injury in DKD patients The study was conducted as a randomized, controlled, prospective study. Patients were recruited over a time period of six months. Overall, 60 DKD patients with control group (n=30) and AG+losartan group (n=30) were included. The characteristics are in Table 1. There were no significantly different between the control group and AG+losartan group (Table 1). With 12 weeks treatment, the ACR in control group and AG+losartan group were significantly lower than 12 weeks ago. Meanwhile, compared to the control group, the ACR were significantly reduced in AG+losartan group (P<0.05). Alanine transaminase (ALT), Aspartic transaminase (AST), Blood urea nitrogen (BUN), Serum creatinine (SCR), Serum uric Acid (SUA), Glomerular filtration rate (GFR) and Kalium (K) were similar among the groups (Table 2). 5 Astragalus granulesinhibited TNF-α and IL-1β generation in DKD patients. ELISA was performed to detect the levels of TNF-α and IL-1β in blood of DKD patients. With 12 weeks treatment, astragalus granules and losartan treatment strikingly inhibited the increase of TNF-α and IL-1β in DKD rats (P<0.05)(Figure 6). But there was no significantly different in control group.
Data for: A trial of in situ and static measurements of levels of radioactive cesium 137 on shallow rugged reefs lying close to the coastline of Fukushima
Contributors: Fumie Suzuki
... Supplementary Data of the article "A trial of in situ and static measurements of levels of radioactive cesium 137 on shallow rugged reefs lying close to the coastline of Fukushima"
Contributors: Olanrewaju Lawal
... Exposure capture factors which could be manifested in the magnitude and intensity of long-term changes in climate (Intergovernmental Panel on Climate Change, 2007) and in this context factors with impact on agricultural production. Temperature and rainfall were used to capture the extent to which Maize is exposed to climate change. Data was sourced from the Centre for Environmental Data Analysis (CRU TS release 4), with data extracted for 1941 - 2015. The data were processed within R (Version 3.4.2), within this environment the mean (temperature and rainfall) for northern and southern parts of the country were computed. The growing season for Maize in the north spans from May to September while in the south it starts from March and ends in August (FAO, 2018). Furthermore, long (1941 – 2015) and short (1961 – 2015) term averages for the respective growing season were computed for each of the regions. Following the computation of the long and short-term averages, exposure was computed as the ratio of the long-term to the short-term averages. With exposure index for rainfall and temperature computed separately, the two were added to get the combined exposure index. A high value indicates high exposure to climate variability. In this dataset, the exposure index is presented in raster format (Geotiff) to allow for easy processing across GIS software. In addition, the boundaries of the northern and the southern regions were also included as shapefiles.
Contributors: Fanling Hong
... Meta-analysis of retrospective case control studies about the risk factors for splenic hilum lymph node metastasis in gastric cancer