Contributors: Joshua Soderholm
... The following collection is used to demonstrate the HailPixel survey technique as part of an AGU GRL publication. The following is an abstract for this paper: A new technique, named "HailPixel," is introduced for measuring the maximum dimension and intermediate dimension of hailstones from aerial imagery. The photogrammetry procedure applies a convolutional neural network for robust detection of hailstones against complex backgrounds and an edge detection method for measuring the shape of identified hailstones. This semi-automated technique is capable of measuring many thousands of hailstones within a single survey, which is several orders of magnitude larger than population sizes from existing sensors (e.g., a hail pad). Comparison with a co-located hail pad for an Argentinan hailstorm event demonstrates the larger population size of the HailPixel survey significantly improves the shape and tails of the observed hail size distribution. When hailfall is sparse, such as during large and giant hail events, the large survey area of this technique is especially advantageous for resolving the hail size distribution. The dataset contains the DEM and orthomosaic imagery, processing reports, final location of hail centroids, final measurements of hail major and minor axis, subset offsets and hail pad data. For more information applying the subset offsets to calculate the true position of the hail centroids please see the paper.
Contributors: Olanrewaju Lawal
... Exposure capture factors which could be manifested in the magnitude and intensity of long-term changes in climate (Intergovernmental Panel on Climate Change, 2007) and in this context factors with impact on agricultural production. Temperature and rainfall were used to capture the extent to which Maize is exposed to climate change. Data was sourced from the Centre for Environmental Data Analysis (CRU TS release 4), with data extracted for 1941 - 2015. The data were processed within R (Version 3.4.2), within this environment the mean (temperature and rainfall) for northern and southern parts of the country were computed. The growing season for Maize in the north spans from May to September while in the south it starts from March and ends in August (FAO, 2018). Furthermore, long (1941 – 2015) and short (1961 – 2015) term averages for the respective growing season were computed for each of the regions. Following the computation of the long and short-term averages, exposure was computed as the ratio of the long-term to the short-term averages. With exposure index for rainfall and temperature computed separately, the two were added to get the combined exposure index. A high value indicates high exposure to climate variability. In this dataset, the exposure index is presented in raster format (Geotiff) to allow for easy processing across GIS software. In addition, the boundaries of the northern and the southern regions were also included as shapefiles.
Data for: Plasmid BMP-2–Embedded Gelatin Sponge As A Gene-Activated Matrix for Preosteoblast Differentiation
Contributors: Po-Liang Lai, Min-Kai Hsieh, Chi-Chien Niu, Tsung-Ting Tsai, Shinn-Chih Wu, Ming-kai Hsieh
... Supplementary Figure 1 To allow endocytosis of DNA complexes into the cells, 5 × 104 cells and TransIT-2020/pEGFP-C1 complexes were mixed for 5, 15, 30, or 60 min (n = 3). Flow cytometry analysis was performed to evaluate GFP expression at these time points using FACS Calibur flow cytometer (Becton Dickinson, Heidelberg, Germany). Cells were gated at 1 × 104 cells/sample in FL1 channel setting, and BMP-2 transfection samples under the same condition at each time point were used as controls(Figure S1A). Transfection efficiencies of these four mixing time points were compared using Becton Dickinson Cell Quest software(Figure S1B). We found that duration of 30 min was the optimal mixing time, as the average GFP expression (9.92%) at 30 min was the highest compared to that at the other three time points. Live and dead assay : The scaffolds were stained using a Live/Dead Cytotoxicity/Viability kit (Invitrogen, Carlsbad, CA)from day 7 to day 28 .The live cells ,dead cells and merge images were all included. Figure 5 ALP supplementary data : all ALP activity corresponding to Figure 5 Figure 6 ARS supplementary data : all ARS activity corresponding to Figure 6 Figure 7 BMP-2 supplementary data: all BMP-2 expression corresponding to Figure 7
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Contributors: Maikol Castro, Dora Maria Ballesteros L, Diego Renza
... This dataset is composed of 100 natural images, 1000 tampered counterparts, and their 1000 images of difference (i.e., tampered minus original). The natural images were captured in public sites like parks, streets, and shopping center. For every image were applied 10 manipulations among copy-move, colorizing and retouching operations.
Data for: FAULT-FLUID INTERACTION IN PORPHYRY COPPER HYDROTHERMAL SYSTEMS: FAULTED VEINS IN RADOMIRO TOMIC, NORTHERN CHILE.
Contributors: erik jensen, Thomas Mitchell, Daniel R. Faulkner, Gabriel Gonzalez, Jose Cembrano
... Raw patterns , Rietveld refinement parameters and quantitative results from X-ray diffraction of fault-related rocks in Radomiro Tomic porphyry copper deposit, Chile Extra set micro-photographies of the analysed rock samples.
Data/Software for "Presynaptic Mitochondria Volumes and Complexity of Subsynaptic Distribution Increase During Development at a High-fidelity Synapse"
Contributors: Connon Thomas, Satoko Okayama, Christian Keine, Rachel Satterfield, Morgan Musgrove, Debbie Guerrero-Given, Naomi Kamasawa, Samuel M. Young, Jr.
... Contains data and software from the publication: "Presynaptic Mitochondria Volumes and Complexity of Subsynaptic Distribution Increase During Development at a High-fidelity Synapse" currently under review. In this study, we created a helper-dependent adenoviral vector (HdAd) to co-express cytoplasmic EGFP and a genetically encoded peroxidase marker (mito-APEX2) at the calyx of Held, an excellent model for deciphering regulatory mechanisms of presynaptic function. ABSTRACT: The calyx of Held, a large glutamatergic presynaptic terminal in the auditory brainstem undergoes developmental changes to support the high action-potential firing rates required for auditory information encoding. In addition, calyx terminals are morphologically diverse which impacts vesicle release properties and synaptic plasticity. Mitochondria influence synaptic plasticity through calcium buffering and are crucial for providing the energy required for synaptic transmission. Therefore, it has been postulated that mitochondrial levels increase during development and contribute to the morphological-functional diversity in the mature calyx. However, the developmental profile of mitochondrial volumes and subsynaptic distribution at the calyx of Held remains unclear. To provide insight on this, we developed a helper-dependent adenoviral vector (HdAd) that expresses the genetically encoded peroxidase marker for mitochondria, mito-APEX, at the mouse calyx of Held. We developed protocols to detect labeled mitochondria for use serial block face SEM (SBF-SEM) to carry out semi-automated segmentation of mitochondria, high-throughput whole terminal reconstruction and presynaptic ultrastructure in mice of either sex. Subsequently, we measured mitochondrial volumes and subsynaptic distributions at the immature postnatal day 7 (P7) and the mature (P21) calyx. We found an increase of mitochondria volumes in terminals and axons from P7 to P21 but did not observe differences between stalk and swelling subcompartments in the mature calyx. Based on these findings, we propose that mitochondrial volumes developmentally increase to support high firing rates but have limited contribution to morphological-functional diversity at the calyx. Data are sorted by the figures they appear in and custom-written software is located in a separate folder.
EXD2 protects stressed replication forks and is required for cell viability in the absence of BRCA1/2. Nieminuszczy et al.
Contributors: Jadwiga Nieminuszczy, Ronan Broderick, Marina Bellani, Elizabeth Smethurst, Rebekka Schwab, Veronica Cherdyntseva, Theodora Evmorfopoulou, Yea-Lih Lin, Michal Minczuk, Philippe Pasero
... Original imaging data related to Nieminuszczy et al. "EXD2 Protects Stressed Replication Forks and Is Required for Cell Viability in the Absence of BRCA1/2”, Molecular Cell (2019) DOI:https://doi.org/10.1016/j.molcel.2019.05.026
Contributors: Jorge Parraga-Alava
... A lemon (Le) leaf (Le) images dataset for aphids (Phid) detection. It includes 665 files with annotations regarding objects (leaves) and state (healthy and aphids presence).
Data for: Reducing the U-Net size for practical scenarios: Virus recognition in electron microscopy images
Contributors: Damian Matuszewski, Ida-Maria Sintorn
... The source code and results of the best U-Net-based model described in the paper.