Data for: Marinitoga lauensis sp. nov., a novel deep-sea hydrothermal vent thermophilic anaerobic heterotroph with a prophage.
Contributors: anna-louise reysenbach, Lena Gouhier, Emily St. John
... Marinitoga lauensis sp. nov., a novel deep-sea hydrothermal vent thermophilic anaerobic heterotroph with a prophage. Stéphane L’Haridona*, Léna Gouhiera, Emily St. Johnb and Anna-Louise Reysenbachb Univ Brest, CNRS, IFREMER, Laboratoire de Microbiologie des Environnements Extrêmes, F-29280, Plouzané, Francea; Portland State University, Department of Biology and Center for Life in Extreme Environments, P.O. Box 751, Portland, OR 97207 USAb.
Contributors: Ying-Jui Hsu
... Chlorine partitioning between granitic melt and C-O-H-Cl aqueous fluids in the Earth’s upper crust, and some implications for magmatic-hydrothermal ore genesis
The NAD-booster nicotinamide riboside potently stimulates hematopoiesis through increased mitochondrial clearance
Contributors: Olaia Naveiras
... It has been recently shown that increased oxidative phosphorylation, as reflected by increased mitochondrial activity, together with impairment of the mitochondrial stress response can severely compromise hematopoietic stem cell (HSC) regeneration. Here we show that the NAD+-boosting agent Nicotinamide Riboside (NR) reduces mitochondrial activity within HSCs through increased mitochondrial clearance, leading to increased asymmetric HSC divisions. NR dietary supplementation results in a significantly enlarged pool of progenitors, without concurrent HSC exhaustion, improves survival by 80%, and accelerates blood recovery after murine lethal irradiation and limiting-HSC transplantation. In immune-deficient mice, NR increased the production of human leucocytes from hCD34+ progenitors. Our work demonstrates for the first time a positive effect of NAD+ boosting strategies on the most primitive blood stem cells, establishes a novel link between HSC mitochondrial stress, mitophagy and stem cell fate decision, and unveils the potential of NR to improve recovery of patients suffering from hematological failure including post-chemo/radiotherapy.
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Data for: Access to acute care resources in various income settings to treat new-onset stroke: a survey of acute care providers
Contributors: Clint Hendrikse, Stevan Bruijns, Ramadhan Chunga
... A self-reported cross-sectional survey was conducted of delegates that attended the April 2016 International Conference on Emergency Medicine using the web-based e-Survey client, Survey Monkey Inc. The survey assessed both pre-hospital and in-hospital settings and was piloted before implementation.
Contributors: Nicolas Sarute, Susan R Ross
... Data from to the manuscript "TRIM2, a novel member of the antiviral family, limits new world arenavirus entry"
Data for: Kinetic, physicochemical properties and effects of pesticides and heavy metals on brain acetylcholinesterase of bentonic fish Hoplosternum littorale: Perspectives of application in aquatic biomonitoring
Contributors: MARIA OLIVEIRA, Rafael Souto Azevedo, Kaline Catiely Campos Silva, Caio Assis, Mônica Lucia Adam, Valdir Luna Silva, Kelma Sirleide Souza, Marlyete Chagas Araujo, Maria Betania Melo Oliveira, Luciano Clemente da Silva
... Highlights 1. Brain ChE from H. littorale was physicochemical and kinetically characterized; 2. Selective inhibitors showed AChE as the main ChE in H. littorale brain; 3. The enzyme was in vitro exposed to eleven pesticides and ten ions; 4. This study presents the first report on the inhibition of cerebral AChE in the Siluriformes group of fish by the pesticides, novaluron and pyriproxyfen; 5. The AChE from H. littorale was highly sensitive to dichlorvos at concentrations (IC20 - IC50) well below the maximum concentrations of this pesticide allowed by the current legislation; 6. Among the ions studied, mercury altered the enzymatic activity most significantly.
Contributors: Richard Zimmermann, Regine Stutz
... In mammalian cells, one-third of all polypeptides are transported into/across the ER-membrane via the Sec61-channel. While the Sec61-complex facilitates translocation of all polypeptides with amino-terminal signal peptides (sp) or transmembrane helices, the Sec61-associated TRAP-complex supports translocation of only a subset, i.e. in a substrate-specific manner. To characterize TRAP-dependent precursors, we combined siRNA-mediated TRAP depletion in human cells, label-free quantitative proteomics, and differential expression analysis. The results were validated by western blotting and quantitative RT-PCR as well as under conditions of over-production of TRAP-dependent precursors and their mutated variants in the presence or absence of MG132, a proteasome inhibitor. Sp analysis of TRAP-clients revealed above-average glycine-plus-proline content as the distinguishing feature. Thus, TRAP acts as an sp-receptor on the ER-membrane’s cytosolic face for precursor polypeptides with high glycine-plus-proline content in their sp and triggers substrate-specific (and regulated) opening of the Sec61-channel by its interaction with the ER-lumenal hinge of Sec61α. The work is about to be published under the title Proteomics reveals signal peptide features determining the client specificity in human TRAP-dependent ER protein import by Duy Nguyen, Regine Stutz, Stefan Schorr, Sven Lang, Stefan Pfeffer, Hudson H. Freeze, Friedrich Förster, Volkhard Helms1 Johanna Dudek & Richard Zimmermann Here we present the original western blot data plus additional SP and TMH analyses, which are presented in a pptx figure and a xlsx table, respectively . Additional physicochemical properties of TRAP clients with SP or TMH. In addition to the hydrophobicity score and glycine/proline (GP) content, we determined the ΔGapp values of SP sequences (a) and transmembrane helices (TMH) (b) with the ΔGapp predictor for TM helix insertion (http://dgpred.cbr.su.se) and plotted these values against the relative count (Density). Glycine and glycine plus proline content of human signal peptides. We used custom scripts to compute the glycine (G) and glycine plus proline (GP) content of human SP sequences from UniProtKB entries. GP content was calculated as the total fraction of glycine and proline in the respective sequence. Notably, 1382 SP of a total of 3513 have a GP content of > 15%, the proposed threshold for TRAP involvement and, therefore, potential regulation by phosphorylation and/or calcium binding by TRAP subunit α.
Data for: INFLUENCE OF DEPOSITION PARAMETERS ON THE STRUCTURE AND MICROSTRUCTURE OF Bi12TiO20 FILMS OBTAINED BY PULSED LASER DEPOSITION
Contributors: luiz gorup
... support information
Pacer is a mediator of mTORC1 and GSK3-TIP60 signaling in regulation of autophagosome maturation and lipid metabolism
Contributors: Xiawei Cheng, Sun Qiming
... Molecular Cell D-18-00349-Original images of Western blot and microscopy images
Contributors: Claudio De Virgilio, Riko Hatakeyama, Marie-Pierre Peli-Gulli, Zehan Hu, Malika Jaquenoud, Guillermo Miguel Garcia Osuna, Alessandro Sardu, Jörn Dengjel
... Online on December 6th, 2018 in : Molecular Cell Print: Molecular Cell 73, 1–14, January 17, 2019 This is the original data set that contains all of the unprocessed microscopy images, gels, and blots used in this manuscript.