Data/Software for "Presynaptic Mitochondria Volumes and Complexity of Subsynaptic Distribution Increase During Development at a High-fidelity Synapse"
Contributors: Connon Thomas, Satoko Okayama, Christian Keine, Rachel Satterfield, Morgan Musgrove, Debbie Guerrero-Given, Naomi Kamasawa, Samuel M. Young, Jr.
... Contains data and software from the publication: "Presynaptic Mitochondria Volumes and Complexity of Subsynaptic Distribution Increase During Development at a High-fidelity Synapse" currently under review. In this study, we created a helper-dependent adenoviral vector (HdAd) to co-express cytoplasmic EGFP and a genetically encoded peroxidase marker (mito-APEX2) at the calyx of Held, an excellent model for deciphering regulatory mechanisms of presynaptic function. ABSTRACT: The calyx of Held, a large glutamatergic presynaptic terminal in the auditory brainstem undergoes developmental changes to support the high action-potential firing rates required for auditory information encoding. In addition, calyx terminals are morphologically diverse which impacts vesicle release properties and synaptic plasticity. Mitochondria influence synaptic plasticity through calcium buffering and are crucial for providing the energy required for synaptic transmission. Therefore, it has been postulated that mitochondrial levels increase during development and contribute to the morphological-functional diversity in the mature calyx. However, the developmental profile of mitochondrial volumes and subsynaptic distribution at the calyx of Held remains unclear. To provide insight on this, we developed a helper-dependent adenoviral vector (HdAd) that expresses the genetically encoded peroxidase marker for mitochondria, mito-APEX, at the mouse calyx of Held. We developed protocols to detect labeled mitochondria for use serial block face SEM (SBF-SEM) to carry out semi-automated segmentation of mitochondria, high-throughput whole terminal reconstruction and presynaptic ultrastructure in mice of either sex. Subsequently, we measured mitochondrial volumes and subsynaptic distributions at the immature postnatal day 7 (P7) and the mature (P21) calyx. We found an increase of mitochondria volumes in terminals and axons from P7 to P21 but did not observe differences between stalk and swelling subcompartments in the mature calyx. Based on these findings, we propose that mitochondrial volumes developmentally increase to support high firing rates but have limited contribution to morphological-functional diversity at the calyx. Data are sorted by the figures they appear in and custom-written software is located in a separate folder.
Contributors: jasvinder pal Singh, Madhav Institute of Technology & Science, Sakshi Arora, UDAY PRATAP SINGH
... Previous research in gait recognition and available dataset focused on single subjects. But in real time environment (such as airport, metro stations etc.) more than one person walk in a group and occlusion factors affects the performance of gait recognition. Therefore, we presented a new dataset which focused dynamic occlusion scenario. We constructed two categories of gait dataset i.e. first, subjects walk in a group (SMVDU-Multi-Gait) and second, subjects walk separately (SMVDU-Single-Gait). The purpose of this dataset to analyze the variations in gait patterns when same subject walk in a group or individually and also to recognize the target subject in Multi-Gait.
Contributors: UnaElsLive Natra, mdgmatest5 live
... RDM - File Type Support 21May2019 ElsCustomer Apart from .u3d all files preview [ .obj / .ply / .vtk / .stl / .ent / .brk / .pdb / .pse / .mol / .mol2 / .cif / .u3d / .dcm / .nii] - .pse is not supported
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Contributors: Guillem Camprodon, Viktor Smari, Dorian Somers
... The Smart Citizen Web is the main front-end website for the Smart Citizen Platform. It provides a visual website where the project environmental sensors can be accessed in near real time to facilitate the exploration of data with other contextual data (maps, keywords) and processed reports. This is especially important towards citizens engaging at each local site having a sense of ownership over a technology intervention has been associated with sustained community engagement. It connects with the Smart Citizen Platform using its REST API. This work is led by IAAC Fab Lab Barcelona team under the framework of iSCAPE (Improving Smart Control of Air Pollution in Europe) project, which is funded by the European Community’s H2020 Programme (H2020-SC5-04-2015) under the Grant Agreement No. 689954. All the authors acknowledge the funding received via the iSCAPE project.
Supplementary material for the PhD thesis of Rickman, J., "Modelling microtubule dynamics and microtubule-motor self-organisation", UCL, 2019.
Contributors: Jamie Rickman
... Simulation configuration files, documentation and supplementary movies.
Contributors: Carlos Palacio
... Raw data for the internal Project 2016210 "Preparation and characterization of magnetic nanoparticles based in ferrites"--> UAN-UdeA.
Contributors: Cian Desmond, Florent Thiebaut, Jimmy Murphy
... An aluminium cross-section approximately 1 m in length was held between 2 flat plates connected to a movable carriage in a wave basin. Under still water conditions, the cross section was moved through the wave basin. Data were collected from an array of load cells in 6 DoF. This process was repeated for four cross sections, each at 4 angles of attack and 7 velocities. These data can be used for all research scientists and engineers working in the fields of fluid mechanics and hydraulics. The data may be used specifically for the validation of numerical models
Contributors: Prashant Jha, Robert Lipton
... We share the data used in publishing the article "Numerical convergence of finite difference approximations for state based peridynamic fracture models", see https://doi.org/10.1016/j.cma.2019.03.024. The data set comprises of raw data produced by computational code, post-processed files, and python script files. We consider finite difference approximation of a nonlinear state-based peridynamic model. We run simulation for two problems. In the first problem, we have a square domain with verticle pre-crack originating from the middle of the bottom edge. We apply a constant velocity boundary condition along the horizontal axis on the bottom layer. In response to the boundary conditions, the crack propagates vertically. The data correspond to three different horizons, 2mm, 4mm, and 8mm. For each horizon, we have three results, each corresponding to mesh size horizon/2, horizon/4, and horizon/8. From the approximate displacement fields, we compute the rate of convergence with respect to mesh size, for each fixed horizon. These are post-processed data and can be found in "postprocessing" folder of Example 1. In the second problem, we consider a rectangle domain which is supported at two regions (left and right) near the bottom edge. On the portion of the top edge, we apply a monotonically increasing in time force in the downward direction. We run simulation when the sample has just one vertical pre-crack originating from the middle of the bottom edge and when the sample has two vertical pre-cracks symmetrically located and originating from the bottom edge. We plot the damage at multiple times and show that the crack propagates upwards in response to applied load. All computations are carried out using an in-house developed code. In this data set, we have not shared the computational code. However, we plan on making the code public in the future. If you are interested in our code and if you have some collaborative ideas please feel free to get in touch.
Contributors: Alessandro Carfì, Francesco Foglino, Barbara Bruno, Fulvio Mastrogiovanni
... The dataset contains over 1000 recordings of human-human handovers collected from 18 volunteers. The recordings refer to 76 test configurations, which consider volunteer's starting position and role, object to pass and experimenter's motion strategy. Data collected come from 6-axis IMU one for each volunteer, Microsoft Kinect for skeleton tracking and a motion capture system. Each recording is also annotated with a video and a questionnaire about the perceived characteristics of the handover.
Contributors: Talha Agcayazi
... This repository contains the supplement files for the HardwareX Journal titled: Multi-axis Stress Sensor Characterization and Testing Platform Authors: Talha Agcayazi*, Marc Foster, Hannah Kausche, Max Gordon, Alper Bozkurt Affiliations: Electric and Computer Engineering, North Carolina State University Contact email: [email@example.com] Abstract: Multi-Axis mechanical stress sensors have become an important tool in numerous fields. To characterize the quality and resolution of a new stress sensor, researchers use test platforms to apply controlled amounts of shear and normal stresses. Since commercially available multi-axis test platforms are expensive, in many cases researchers end up needing to build their own sensor characterization platforms. In this study, we describe a cost-effective multi-axis stress sensor characterization platform to reduce development time, enable accurate benchmarking and provide an open source standard for stress sensor characterization for other researchers. In our design, we combined a 3-axis translational stage and a 3-axis force and torque (F/T) sensor through custom hardware and software. Our platform is cost effective and can reach the limits of the F/T sensor (Fxy = ±65N and Fz = ±200N) without losing any accuracy to structural bending. We provide detailed construction and operation instructions as well as results of extensive static and cyclic load experiments. To further validate our system, we show characterization results from a custom stress sensor as a case study. The modular nature of our platform also enables other researchers to customize this characterization platform for their unique experimental requirements.