Contributors: Wei-hsiu Liu, Jang-Chun Lin, Jo-Ting Tsai, Tsu-Yi Chao, HSINI MA
... Left: Expression of MSI1, ICAM1, and LIN28 in GBM-U87MG/Ctrl, and GBM-U87MG/MSI1. Right: Expression of MSI1, ICAM1, and LIN28 in GBM-05MG/sh-Scr and GBM-05MG/sh-MSI1 cells. ß-actin was used as a loading control.
Data for: Marinitoga lauensis sp. nov., a novel deep-sea hydrothermal vent thermophilic anaerobic heterotroph with a prophage.
Contributors: anna-louise reysenbach, Lena Gouhier, Emily St. John
... Marinitoga lauensis sp. nov., a novel deep-sea hydrothermal vent thermophilic anaerobic heterotroph with a prophage. Stéphane L’Haridona*, Léna Gouhiera, Emily St. Johnb and Anna-Louise Reysenbachb Univ Brest, CNRS, IFREMER, Laboratoire de Microbiologie des Environnements Extrêmes, F-29280, Plouzané, Francea; Portland State University, Department of Biology and Center for Life in Extreme Environments, P.O. Box 751, Portland, OR 97207 USAb.
Contributors: Christopher Bretherton
... Map of collaborators
Data for: Seipin deletion in mice enhances phosphorylation and aggregation of tau protein through reduced neuronal PPARγ and insulin resistance
Contributors: Ling Chen
... We attached the independent replicates of Western blot analyses in the supplementary section that were used in various quantifications. The regions of line rectangles in these blots/gels, as representative images, were used in Figures of the main Text. In the Fig. 1e: the levels of hippocampal NF-H protein in seipin-sKO mice (sKO) and seipin-nKO mice (nKO) were lower than those of WT mice. In the Fig. 2a: the levels of tau protein in hippocampus of seipin-sKO mice, seipin-nKO mice and seipin-aKO mice (aKO) were not altered. In the Fig. 2b: the levels of oligomer tau protein were increased in seipin-sKO mice and seipin-nKO mice. In the Fig. 2c: the levels of hippocampal tau phosphorylation at Thr212/Ser214 and Ser202/Thr205 were increased in seipin-sKO mice and seipin-nKO mic. In the Fig. 3a: the levels of PPARγ protein were decreased in hippocampus of seipin-sKO mice and seipin-nKO mice. In the Fig. 3b: the administration of PPARγ agonist rosiglitazone (rosi) for 7 days could correct the increased tau phosphorylation at Thr212/Ser214 and Ser202/Thr205 in seipin-sKO mice and seipin-nKO mice. In the Fig. 4a: seipin-sKO mice and seipin-nKO mice showed an increase in the GSK3β phosphorylation at Tyr21 and a decrease at Ser9, which were corrected by rosi. In the Fig. 4b: the treatment with AR-A014418 (AR) corrected the increased tau phosphorylation at Thr212/Ser214 and Ser202/Thr205 in seipin-nKO mice. In the Fig. 5a&5b: the phosphorylation of Akt and mTOR was elevated in seipin-sKO mice and seipin-nKO mice, which were corrected by rosi. In the Fig. 5c&5d: seipin-sKO mice and seipin-nKO mice showed a decrease in the ratio of LC3II/I and an elevation of p62 protein, which was rescued by the PI3K inhibitor LY294002 (LY) and mTOR inhibitor rapamycin (Rap), but not AR. In the Fig. 5e&5f: the administration of LY and Rap reduced the levels of oligomer tau protein and tau phosphorylation at Thr212/Ser214 and Ser202/Thr205 in seipin-nKO mice. In the Fig. 6c: the increased phosphorylation of JNK in seipin-sKO mice were corrected by treatment with rosi for 30 days. In the Fig. 6d: the P38 phosphorylation in seipin-sKO mice was not altered. In the Fig. 6e: the increased phosphorylation of tau at Ser396 in seipin-sKO mice was prevented by treatment with rosi for 30 days and the JNK inhibitor SP600125 (SP).
Data for: miR-196b inhibits cell proliferation, migration and invasion via MAP3K1 in hydatidiform mole
Contributors: Ying Kong, jia qi, na zou, yuefei xu, yunpeng xie, zhenzhen guo, linlin sui, Qiannan Sun Sun
... Raw data of western blot involved in the article
Contributors: Amin Namazi
... By review, both a basic knowledge of earth geology and with understanding In physics and chemistry, the law of conservation of energy, the basic fundamental of agriculture “It's Not the Harvest You Reap But the Seeds You Sow” so we should reach to the solution of the Earth-saving Method.
Contributors: Ying-Jui Hsu
... Chlorine partitioning between granitic melt and C-O-H-Cl aqueous fluids in the Earth’s upper crust, and some implications for magmatic-hydrothermal ore genesis
Contributors: Anthony Roccisano
... The data presented was developed through analysis of EBSD datasets of SCC cracks taken from active low carbon steel pipelines. Samples were analysed on an FEI Inspect F50 SEM with an EDAX Hikari EBSD detector using OIM Data Collection 7 and analysed using OIM Analysis 7. Samples were obtained from the ND-TD plane to capture the morphology of the cracks and the resulting dataset rotated in software by 90 degrees about the TD direction to align the orientation of the sample to that of the system. The data contains information of grain boundary characteristics exported from standard OIM Analysis functions as well as some mechanical properties conducted on the sectioned steel samples.
The NAD-booster nicotinamide riboside potently stimulates hematopoiesis through increased mitochondrial clearance
Contributors: Olaia Naveiras
... It has been recently shown that increased oxidative phosphorylation, as reflected by increased mitochondrial activity, together with impairment of the mitochondrial stress response can severely compromise hematopoietic stem cell (HSC) regeneration. Here we show that the NAD+-boosting agent Nicotinamide Riboside (NR) reduces mitochondrial activity within HSCs through increased mitochondrial clearance, leading to increased asymmetric HSC divisions. NR dietary supplementation results in a significantly enlarged pool of progenitors, without concurrent HSC exhaustion, improves survival by 80%, and accelerates blood recovery after murine lethal irradiation and limiting-HSC transplantation. In immune-deficient mice, NR increased the production of human leucocytes from hCD34+ progenitors. Our work demonstrates for the first time a positive effect of NAD+ boosting strategies on the most primitive blood stem cells, establishes a novel link between HSC mitochondrial stress, mitophagy and stem cell fate decision, and unveils the potential of NR to improve recovery of patients suffering from hematological failure including post-chemo/radiotherapy.