CELL-REPORTS-D-17-02899R1, Rayman et al. "TIA-1 self-multimerization, phase separation, and recruitment into stress granules are dynamically regulated by Zn2+"
Contributors: Joseph Rayman
... These data files are associated with the following article: Rayman, J.B., Karl, K.A., and Kandel, E.R. (2018). TIA-1 self-multimerization, phase separation, and recruitment into stress granules are dynamically regulated by Zn2+. Cell Reports 22, 1-13.
The Ebola virus nucleoprotein recruits the host PP2A-B56 phosphatase to activate transcriptional support activity of VP30
Contributors: Jakob Nilsson, Thomas Kruse, Nadine Biedenkopf, Stephan Becker, Emil Hertz, Erik Dietzel, Norman Davey, Blanca López-Méndez, Gertrud Stallman
... Datapoints, uncropped blots and images, raw ITC data and sequences of oligonucleotides used.
Contributors: Yungang Liu
... Raw experimental data and associate plots, primarily including results of micronucleus tests, Hprt mutagenicity assays, CCK-8 cytotoxicity assays, q-PCR, and Westernblotting assays.
Contributors: Tariq rahmani
... SCCP Routing solution for "No translation for this specific address" Error
Contributors: Min-Dian Li
... Original data of images and Western blot. Cell Reports (2017), https://doi.org/10.1016/j.celrep.2017.09.051
Data for: Study of the non-clinical healing activities of the extract and gel of Portulaca pilosa L. in skin wounds in wistar rats
Contributors: José Carlos Tavares Carvalho, jonatas duarte, Helison Carvalho, ALBENISE ALVES, Igor Victor dos Santos, Tafnis Ingret Sampaio, Caio Fernandes, Talisson Taglialegna
... Introduction: Portulaca pilosa L., belonging to the family Portulacaceae, is a common herbaceous plant in the Americas and in the Amazon, is popularly known as love-grown and is traditionally used as an aid in the treatment of burns, buds, insect bites and wound healing. This study aims to evaluate the non-clinical topical healing activity of the P. pilosa gel (GPP) and the propyleneglycol extract of P. pilosa (EPP) in Wistar rats. Methods: For the healing activity, wistar rats were divided into the following groups: negative control (GVE - vehicle, 150 mg/kg), positive control (Fibrinase®- FIB, 100 U/kg), Portulaca pilosa gel 10%, (GPP, 150 mg/kg), and propylenglycollic extract of Portulaca pilosa (EPP, 150 mg/kg), which were submitted to a surgical procedure to obtain the wounds, and were treated topically for 7 days. After treatment, the treated area was removed and a histopathological analysis was performed. Results: The EPP when analyzed in HPLC was able to identify the presence of gallic acid. EPP significantly modulated the tissue inflammatory response, presenting low number of inflammatory cells in the histopathological study. Treatment with EPP and GPP significantly stimulated angiogenesis and this response was superior to the fibrinase® group. Treatment with EPP and GPP significantly stimulated the proliferation of fibroblasts. The groups treated with EPP and GPP presented an organization pattern of the epidermis and dermis better than the control group, with a mild inflammatory process, with fibroblast proliferation and increased formation of collagen fibers. Conclusion: Thus, from the results obtained it can be suggested that the phytochemical marker of the P. pilosa species for healing activity is gallic acid and, together with the macroscopic and microscopic findings triggered by the topical applications of EPP and GPP, it can be concluded that this plant species has topical healing activity, with great potential for use, since this pharmacological action is associated with a possible topical anti-inflammatory activity.
Contributors: Christelle Larzabal, Eve Tramoni, Sophie Muratot, Simon Thorpe, Emmanuel Barbeau
... ## RESULTS ________ Task1: Forced-choice for TEST participants: filename: classeur'x'_set'y'Choix_forcé_pp_'z'_TESTMarseille.xlsx Forced-choice for CONTROL participants: filename: classeur'x'_set'y'Choix_forcé_pp_'z'_CTRL.xlsx Task2: Yes/No for TEST participants: filename: Oui_Non_pp'z'_TESTMarseille.xlsx ## MATERIAL ________ # BINDERS A binder was used for each participant including three different sets. Power point file of the binders: classeur'x'_set'y'.pptx # STIMULI PNG file starting by "C_": stimuli seen at least 3 times in the initial DMS-48 PNG file starting by "D1_": stimuli seen once in the initial DMS-48 during the first recognition test performed 3 min after the incidental encoding PNG file starting by "D2_": stimuli seen once in the initial DMS-48 during the second recognition test performed 1h after the incidental encoding PNG file starting by "LC_", "LD1_" and "LD2_" : the lures of the respective stimuli that are found in the precedent folders.
Additional Files for "Characterizing the loblolly pine (Pinus taeda L.) genome by BAC sequencing and Cot analysis"
Contributors: Daniel Peterson
... Additional File 1. Detailed protocols: (A) Isolation of nuclear DNA from plants; (B) Dialysis of nucleic acid solutions; (C) Shearing DNA into 450 bp fragments using the Misonix sonicator 3000; (D) Removing metal ions from DNA solutions using Chelex; (E) Preparing 0.5 m sodium phosphate buffer (SPB); (F) Preparing ASE buffer (PDF 744 kb). Additional File 2. List of targeted genes (carbon metabolism, wood development, transcription factor, signaling, or disease resistance), their references, and the overgo probe sequences used in screening. A total of seventy-three overgo probes were used to screen BAC macroarrays: twenty-five (colored in blue) were included in a first batch of screening while the last forty-eight (colored in green) were utilized in a second screen (XLS 41 kb). Additional File 3. Schematic representation of the screening steps performed to identify the targeted BACs: (A) screening outline (B) Pooling pattern (PPTX 46 kb). Additional File 4. Examples of screened 4 x 4 macroarrays from the first (A) and the second (B) batches of screening. The coordinates of the positive clones on the macroarrays correspond to the well position in the 384-well plate while the configuration of the double spots corresponds to the plate number of the 384-well plate where the clones are located. Panel C shows hybridization with repetitive elements and D shows NotI digestion of the identified low-copy BACs from C to verify the presence of inserts (PPTX 876 kb). Additional File 5. List of the BAC clones that were selected for sequencing, the corresponding GenBank accession numbers, gene index, length of assembly, type of BAC clone, the probe used to screen the targeted BAC clones, notes, LP draft genome scaffold ID, and percent query coverage. A total of 100 BAC clones were sequenced: 50 targeted BAC clones, 25 low-copy sequence BAC clones, and 25 random BAC clones (XLS 48 kb).
Laguesse_Prosapip1-Dependent Synaptic Adaptations In The Nucleus Accumbens Drive Alcohol Intake, Seeking, And Reward
Contributors: sophie laguesse
... Prosapip1-Dependent Synaptic Adaptations in the Nucleus Accumbens Drive Alcohol Intake, Seeking, and Reward Sophie Laguesse, Nadege Morisot6,7, Jung Hoon Shin6, Feng Liu8, Martin F. Adrover, Samuel A. Sakhai, Marcelo F. Lopez, Khanhky Phamluong, William C. Griffin III, Howard C. Becker, Kevin J. Bender, Veronica A. Alvarez, Dorit Ron DOI: http://dx.doi.org/10.1016/j.neuron.2017.08.037
Contributors: charles latchoumane, Hong Viet Ngo
... Average traces and phase plot of stimulation for each mice of each group for the manuscript: "Spindles promote memory formation during NREM through triple phase-locking of cortical, thalamic and hippocampal rhythms," Latchoumane et al.