Contributors: Eran Halfi, Asher Brenner, David Katoshevski
... This video, filmed with Go-Pro Hero2, is an overview of the grouping process in a laboratory experiment conducted in a 2 Liter beaker with initial turbidity of 50 NTU. The time shown in the down right corner of the presentation is in minutes (high speed editing). The experiment consisted of 30 minutes of oscillations. The white sedimentation which accumulates in the bottom of the beaker are the aggregates which are formed and settle due to the oscillation process in distinct settling zones. The shape of "lungs" formed by the aggregates sedimentation is representative for all experiments.
Contributors: Uma Nagarajan
Contributors: Saroj Chakraborty
... Salt-responsive Metabolite, beta-hydroxybutyrate, Attenuates Hypertension Chakraborty, S1, Galla, S1, Cheng X1, Yeo, J1, Mell, B1, Singh V1, Yeoh BS1, Saha P1, Mathew, AV2, Vijay-Kumar, M1, Joe B*1. 1Program in Physiological Genomics, Microbiome Consortium, Center for Hypertension and Personalized Medicine, Department of Physiology and Pharmacology, University of Toledo College of Medicine and Life Sciences, Toledo, OH, USA. 2 Department of Internal Medicine-Nephrology, University of Michigan, Ann Arbor, MI, USA. *Corresponding Author: Bina Joe, email@example.com Summary Dietary salt reduction and exercise are lifestyle modifications prescribed for salt-sensitive hypertensives. While exercise is known to have prominent metabolic effects, such effects of lower dietary salt are just emerging. Studies indicate that salt has adverse effect on metabolic syndrome, of which, hypertension is a hallmark. We hypothesized that dietary salt impacts metabolism in a salt-sensitive model of hypertension. Untargeted metabolomic profiling of rats with differential salt (NaCl) diets revealed that a high salt-induced increase in blood pressure was associated with lower circulating levels of the ketone body, beta-hydroxybutyrate (βOHB). Specific rescue of the βOHB levels by nutritional supplementation of its precursor, 1,3-butanediol attenuated hypertension and protected kidney disease despite the high salt intake. This beneficial effect of βOHB was observed independent of the gut-microbiotal effects of salt or the Th17-mediated renal effects. Instead, the mechanism for the observed renoprotective effect of βOHB on salt-sensitive hypertension was the βOHB-mediated inhibition of the pro-inflammatory cascade by the Nlrp3 inflammasome. In summary, the juxtaposed effects of dietary salt and exercise on salt-sensitive hypertension, which decrease and increase βOHB respectively, indicates that nutritional supplementation of a precursor of βOHB could be considered to provide a similar benefit to salt-sensitive hypertension as exercise.
an approach to improve the mechanical properties of LM24 alloy via dislocation enhanced precipitates
Contributors: yijie zhang
... The main idea is to develop an approach to improve the mechanical properties of LM24 alloy by the enhanced the precipitates strengthening when carrying on the directly ageing treatment. To achieve this, the formation of precipitates is promoted by the dislocation movement and to optimize the size of the precipitates by accelerating the diffusion along dislocation.
Contributors: Alessandro Borgognone
... Background, surgery and perspectives of the oncoplastic breast surgery are examined and discussed .
Contributors: Hsiang-Chen Chui, Wei-Chen Lin, Yi-Hsi Chen, Jowtsong Shy, Hong-Zui Chen
... The Supplement information of this research work. It included the optical layout, the spectra analysis under different temperatures, and applied currents.
Data for: All-trans retinoic acid ameliorates inflammatory response mediated by TLR4/NF-кB during initial of diabetic nephropathy
Contributors: Jose L. Reyes, Maria del Carmen Namorado-Tónix, Rafael Rodríguez-Muñoz, José Pedraza-Chaverri, Eduardo Molina-Jijón, Edith Sierra-Mondragón
... The raw data showed in this article comes from the article entitled “All-trans retinoic acid ameliorates inflammatory response mediated by TLR4/NF-кB during the initiation of diabetic nephropathy”. Data illustrates the beneficial effect of ATRA in diabetes-induced early inflammation. Anti-inflammatory effect was evaluated in cortex renal, glomeruli, proximal and distal tubules through Western blot assay.
Contributors: Richard Zimmermann, Marie-Christine Klein
... The endoplasmic reticulum (ER) requires a constant ATP supply. However, the carrier that catalyzes ATP uptake into the ER was unknown. In search of the elusive ATP/ADP carrier we screened gene expression datasets for member(s) of the family of solute carriers that are co-expressed with BiP, the major ER resident ATP consumer, and are ER membrane proteins. We identified SLC35B1 as a potential candidate. Heterologous expression of SLC35B1 in E. coli revealed that SLC35B1 is highly specific for ATP and ADP and acts in antiport mode. Moreover, siRNA mediated depletion of SLC35B1 from HeLa cells reduced ER ATP levels and BiP activity. Thus, human SLC35B1 provides ATP to the ER and was named AXER (ATP/ADP exchanger in the ER membrane). Furthermore, we began to unravel an ER to cytosol low energy response regulatory axis (termed lowER) that is central for maintaining ER ATP supply under steady state and stress conditions. This work is about to be published under the title AXER is an ATP/ADP exchanger in the membrane of the endoplasmic reticulum by Marie-Christine Klein, Katharina Zimmermann, Stefan Schorr, Martina Landini, Patrick A. W. Klemens, Jacqueline Altensell, Martin Jung, Elmar Krause, Duy Nguyen, Volkhard Helms, Jens Rettig, Claudia Fecher-Trost, Adolfo Cavalié, Markus Hoth, Ivan Bogeski, H. Ekkehard Neuhaus, Richard Zimmermann, Sven Lang and Ilka Haferkamp Here we present the original western blot data.
Contributors: Agnieszka Tudek
... A nuclear export block triggers the decay of newly synthesized polyadenylated RNA Agnieszka Tudek1, Manfred Schmid1, Marius Makaras1, J. David Barrass2, Jean D. Beggs2, Torben Heick Jensen1* 1Department of Molecular Biology and Genetics, Aarhus University, C. F. Møllers Allé 3, building 1130, 8000 Aarhus C, Denmark, 2Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, UK *corresponding author and lead contact: firstname.lastname@example.org Keywords: nuclear export of pA+ RNA, nuclear degradation of pA+ RNA, Mex67p, Nab2p, transcription. ABSTRACT Genomes are promiscuously transcribed necessitating mechanisms that facilitate the sorting of RNA for function or destruction. The polyA (pA) tail is one such distinguishing feature, which in the Saccharomyces cerevisiae nucleus is bound by the Nab2p protein, yielding transcript protection. As Nab2p also contacts the main nuclear export factor Mex67p, we asked whether transport kinetics contributes to RNA sorting. Indeed, 3’end sequencing of newly transcribed pA+ RNAs demonstrates that nuclear depletion of Mex67p elicits their instant and global decay. A similar phenotype is evident upon inactivation of other export factors and proportional to the amount of nuclear pA+ RNA. As RNA expression is partially rescued by Nab2p over-expression, we propose that an export-block out-titrates Nab2p onto nuclear retained pA+ RNA, reducing the pool of Nab2p available to protect new transcripts. More generally, we suggest that nuclear RNA decay, negotiated by Nab2p availability, aids in balancing cellular transcript supply with demand.
Contributors: Xuping Xie
... As shown in Fig. 3h, the C7a/t virus contained two C variants: HA-C (~12.5 kDa) and C7 (~10.5 kDa) proteins with an estimated molecular ratio of 1:30, whereas the WT ZIKV had only full-length C protein (~11.4 kDa). As expected, both C7a/t and WT viruses contained mature M and E proteins. As controls, culture medium from replicon-transfected cells contained only NS1 protein, with no detectable structural proteins (C, M, or E). The data suggest that C7a/t ZIKV contains both HA-C and C7 proteins. Western blot analysis of C7a/t virus. Supernatants from replicon RNA-transfected BHK-HA-C cells (day 4 p.t.), WT RNA-transfected Vero cells (day 4 p.t.), and C7a RNA-transfected BHK-HA-C cells (day 4 p.t.) were analyzed for viral proteins (HA-C, C, M, E, and NS1) using Western blotting.