Data for: Kinetic, physicochemical properties and effects of pesticides and heavy metals on brain acetylcholinesterase of bentonic fish Hoplosternum littorale: Perspectives of application in aquatic biomonitoring
Contributors: MARIA OLIVEIRA, Rafael Souto Azevedo, Kaline Catiely Campos Silva, Caio Assis, Mônica Lucia Adam, Valdir Luna Silva, Kelma Sirleide Souza, Marlyete Chagas Araujo, Maria Betania Melo Oliveira, Luciano Clemente da Silva
... Highlights 1. Brain ChE from H. littorale was physicochemical and kinetically characterized; 2. Selective inhibitors showed AChE as the main ChE in H. littorale brain; 3. The enzyme was in vitro exposed to eleven pesticides and ten ions; 4. This study presents the first report on the inhibition of cerebral AChE in the Siluriformes group of fish by the pesticides, novaluron and pyriproxyfen; 5. The AChE from H. littorale was highly sensitive to dichlorvos at concentrations (IC20 - IC50) well below the maximum concentrations of this pesticide allowed by the current legislation; 6. Among the ions studied, mercury altered the enzymatic activity most significantly.
The effects of pH, adsorption dosage and initial per- and polyfluoroalkyl substances (PFAS) concentrationon on the adsorption mechanism of PFAS using maize tassel (MT) and chemically activated maize tassel (CAMT) as adsorbents.
Contributors: Patricia Omo-Okoro
... The adsorption of PFAS using novel adsorbents such as maize tassel and activated carbon produced from maize tassel were evaluated via batch equilibrium experiments investigating the effects of pH, adsorbent dosage and initial contaminant concentration. The findings from these experiments were presented at the 38th International Symposium on Halogenated Persistent Organic Pollutants (DIOXIN 2018) Krakow, Poland in August, 2018. The powerpoint slides of the presentation are attached herein.
Contributors: Petter Ström, Per Petersson, Anna Widdowson, Gennady Sergienko, Elżbieta Fortuna-Zaleśna, Marek Rubel
... ToF-ERDA data with partial GIC energy signals from QMB covers 1,2,3,5 (ILW-1-2). Data provided as list-files (.lst) and histogram files (.mpa) from FAST MPA-3 data acquisition system. ADC 1: Energy signal anode 1. ADC 2: Energy signal anodes 2-4. ADC 3: ToF signal.
Contributors: Pushkar Kulkarni, Yogeeswari Perumal, Rita Rani, Raghavender Medishetti, DRILS Hyderabad, Shubham Dwivedi
... All the files attached here are described in the Supplementary Information PDF.
Supplementary material (Contactless high-speed eddy current inspection of unidirectional carbon fiber reinforced polymer)
Contributors: Miguel Machado
... EC density, EC vectors and secondary magnetic field for different ECT probes operation modes.
Contributors: Richard Zimmermann, Regine Stutz
... In mammalian cells, one-third of all polypeptides are transported into/across the ER-membrane via the Sec61-channel. While the Sec61-complex facilitates translocation of all polypeptides with amino-terminal signal peptides (sp) or transmembrane helices, the Sec61-associated TRAP-complex supports translocation of only a subset, i.e. in a substrate-specific manner. To characterize TRAP-dependent precursors, we combined siRNA-mediated TRAP depletion in human cells, label-free quantitative proteomics, and differential expression analysis. The results were validated by western blotting and quantitative RT-PCR as well as under conditions of over-production of TRAP-dependent precursors and their mutated variants in the presence or absence of MG132, a proteasome inhibitor. Sp analysis of TRAP-clients revealed above-average glycine-plus-proline content as the distinguishing feature. Thus, TRAP acts as an sp-receptor on the ER-membrane’s cytosolic face for precursor polypeptides with high glycine-plus-proline content in their sp and triggers substrate-specific (and regulated) opening of the Sec61-channel by its interaction with the ER-lumenal hinge of Sec61α. The work is about to be published under the title Proteomics reveals signal peptide features determining the client specificity in human TRAP-dependent ER protein import by Duy Nguyen, Regine Stutz, Stefan Schorr, Sven Lang, Stefan Pfeffer, Hudson H. Freeze, Friedrich Förster, Volkhard Helms1 Johanna Dudek & Richard Zimmermann Here we present the original western blot data plus additional SP and TMH analyses, which are presented in a pptx figure and a xlsx table, respectively . Additional physicochemical properties of TRAP clients with SP or TMH. In addition to the hydrophobicity score and glycine/proline (GP) content, we determined the ΔGapp values of SP sequences (a) and transmembrane helices (TMH) (b) with the ΔGapp predictor for TM helix insertion (http://dgpred.cbr.su.se) and plotted these values against the relative count (Density). Glycine and glycine plus proline content of human signal peptides. We used custom scripts to compute the glycine (G) and glycine plus proline (GP) content of human SP sequences from UniProtKB entries. GP content was calculated as the total fraction of glycine and proline in the respective sequence. Notably, 1382 SP of a total of 3513 have a GP content of > 15%, the proposed threshold for TRAP involvement and, therefore, potential regulation by phosphorylation and/or calcium binding by TRAP subunit α.
Contributors: Jiaqiang Wang
... The original pictures for the article in https://www.cell.com/cell/fulltext/S0092-8674(18)31564-2?from=timeline&isappinstalled=0
Data for: INFLUENCE OF DEPOSITION PARAMETERS ON THE STRUCTURE AND MICROSTRUCTURE OF Bi12TiO20 FILMS OBTAINED BY PULSED LASER DEPOSITION
Contributors: luiz gorup
... support information
Pacer is a mediator of mTORC1 and GSK3-TIP60 signaling in regulation of autophagosome maturation and lipid metabolism
Contributors: Xiawei Cheng, Sun Qiming
... Molecular Cell D-18-00349-Original images of Western blot and microscopy images
Data for: Electronic model «FC-FNS» of the fusion neutron source DEMO-FNS fuel cycle and modeling hydrogen isotopes flows and inventories in fueling system
Contributors: Sergey Ananyev
... The key task of the fuel cycle (FC) design is to create a closed system providing the conditions for fusion burning in plasma, with reduce tritium inventory in the system elements. For this purpose “FC-FNS” electronic model has been actively developing as a tool for estimating fluxes of DT fuel and T inventories in systems where tritium is used. The paper describe the conceptual fuel cycle design for the DEMO-FNS facility and results of the modeling of hydrogen isotopes flows and inventories in fuel systems for steady-state operation mode.