Data for: Study of the non-clinical healing activities of the extract and gel of Portulaca pilosa L. in skin wounds in wistar rats
Contributors: José Carlos Tavares Carvalho, jonatas duarte, Helison Carvalho, ALBENISE ALVES, Igor Victor dos Santos, Tafnis Ingret Sampaio, Caio Fernandes, Talisson Taglialegna
... Introduction: Portulaca pilosa L., belonging to the family Portulacaceae, is a common herbaceous plant in the Americas and in the Amazon, is popularly known as love-grown and is traditionally used as an aid in the treatment of burns, buds, insect bites and wound healing. This study aims to evaluate the non-clinical topical healing activity of the P. pilosa gel (GPP) and the propyleneglycol extract of P. pilosa (EPP) in Wistar rats. Methods: For the healing activity, wistar rats were divided into the following groups: negative control (GVE - vehicle, 150 mg/kg), positive control (Fibrinase®- FIB, 100 U/kg), Portulaca pilosa gel 10%, (GPP, 150 mg/kg), and propylenglycollic extract of Portulaca pilosa (EPP, 150 mg/kg), which were submitted to a surgical procedure to obtain the wounds, and were treated topically for 7 days. After treatment, the treated area was removed and a histopathological analysis was performed. Results: The EPP when analyzed in HPLC was able to identify the presence of gallic acid. EPP significantly modulated the tissue inflammatory response, presenting low number of inflammatory cells in the histopathological study. Treatment with EPP and GPP significantly stimulated angiogenesis and this response was superior to the fibrinase® group. Treatment with EPP and GPP significantly stimulated the proliferation of fibroblasts. The groups treated with EPP and GPP presented an organization pattern of the epidermis and dermis better than the control group, with a mild inflammatory process, with fibroblast proliferation and increased formation of collagen fibers. Conclusion: Thus, from the results obtained it can be suggested that the phytochemical marker of the P. pilosa species for healing activity is gallic acid and, together with the macroscopic and microscopic findings triggered by the topical applications of EPP and GPP, it can be concluded that this plant species has topical healing activity, with great potential for use, since this pharmacological action is associated with a possible topical anti-inflammatory activity.
Contributors: Christelle Larzabal, Eve Tramoni, Sophie Muratot, Simon Thorpe, Emmanuel Barbeau
... ## RESULTS ________ Task1: Forced-choice for TEST participants: filename: classeur'x'_set'y'Choix_forcé_pp_'z'_TESTMarseille.xlsx Forced-choice for CONTROL participants: filename: classeur'x'_set'y'Choix_forcé_pp_'z'_CTRL.xlsx Task2: Yes/No for TEST participants: filename: Oui_Non_pp'z'_TESTMarseille.xlsx ## MATERIAL ________ # BINDERS A binder was used for each participant including three different sets. Power point file of the binders: classeur'x'_set'y'.pptx # STIMULI PNG file starting by "C_": stimuli seen at least 3 times in the initial DMS-48 PNG file starting by "D1_": stimuli seen once in the initial DMS-48 during the first recognition test performed 3 min after the incidental encoding PNG file starting by "D2_": stimuli seen once in the initial DMS-48 during the second recognition test performed 1h after the incidental encoding PNG file starting by "LC_", "LD1_" and "LD2_" : the lures of the respective stimuli that are found in the precedent folders.
Additional Files for "Characterizing the loblolly pine (Pinus taeda L.) genome by BAC sequencing and Cot analysis"
Contributors: Daniel Peterson
... Additional File 1. Detailed protocols: (A) Isolation of nuclear DNA from plants; (B) Dialysis of nucleic acid solutions; (C) Shearing DNA into 450 bp fragments using the Misonix sonicator 3000; (D) Removing metal ions from DNA solutions using Chelex; (E) Preparing 0.5 m sodium phosphate buffer (SPB); (F) Preparing ASE buffer (PDF 744 kb). Additional File 2. List of targeted genes (carbon metabolism, wood development, transcription factor, signaling, or disease resistance), their references, and the overgo probe sequences used in screening. A total of seventy-three overgo probes were used to screen BAC macroarrays: twenty-five (colored in blue) were included in a first batch of screening while the last forty-eight (colored in green) were utilized in a second screen (XLS 41 kb). Additional File 3. Schematic representation of the screening steps performed to identify the targeted BACs: (A) screening outline (B) Pooling pattern (PPTX 46 kb). Additional File 4. Examples of screened 4 x 4 macroarrays from the first (A) and the second (B) batches of screening. The coordinates of the positive clones on the macroarrays correspond to the well position in the 384-well plate while the configuration of the double spots corresponds to the plate number of the 384-well plate where the clones are located. Panel C shows hybridization with repetitive elements and D shows NotI digestion of the identified low-copy BACs from C to verify the presence of inserts (PPTX 876 kb). Additional File 5. List of the BAC clones that were selected for sequencing, the corresponding GenBank accession numbers, gene index, length of assembly, type of BAC clone, the probe used to screen the targeted BAC clones, notes, LP draft genome scaffold ID, and percent query coverage. A total of 100 BAC clones were sequenced: 50 targeted BAC clones, 25 low-copy sequence BAC clones, and 25 random BAC clones (XLS 48 kb).
Laguesse_Prosapip1-Dependent Synaptic Adaptations In The Nucleus Accumbens Drive Alcohol Intake, Seeking, And Reward
Contributors: sophie laguesse
... Prosapip1-Dependent Synaptic Adaptations in the Nucleus Accumbens Drive Alcohol Intake, Seeking, and Reward Sophie Laguesse, Nadege Morisot6,7, Jung Hoon Shin6, Feng Liu8, Martin F. Adrover, Samuel A. Sakhai, Marcelo F. Lopez, Khanhky Phamluong, William C. Griffin III, Howard C. Becker, Kevin J. Bender, Veronica A. Alvarez, Dorit Ron DOI: http://dx.doi.org/10.1016/j.neuron.2017.08.037
Contributors: charles latchoumane, Hong Viet Ngo
... Average traces and phase plot of stimulation for each mice of each group for the manuscript: "Spindles promote memory formation during NREM through triple phase-locking of cortical, thalamic and hippocampal rhythms," Latchoumane et al.
Contributors: Gilberto Capistrano Cunha de Andrade, Dário Ferreira
... Curso Extensão
Data for: Quantitative Analysis of Nucleic Acid-Metal Interactions and their Biological Implications
Contributors: Rachel Hellmann-Whitaker, Aleksandar Dimkovikj, Mallory Banton, Elizabeth Thorn, Lance McDanel, Katelin Arndt, Ana Royo Marco, Katelyn Unvert
... This data is a comprehensive quantitative analysis of nucleic acid-heavy metal interactions. Our abstract is as follows: RNA is known to interact with Mg2+ when assuming higher-ordered tertiary configurations. Structurally, when tRNA molecules interact with Mg2+, they consistently form a “L-shape” conformation each time they are synthesized. Therefore, if Mg2+ can induce tertiary structure formation, then binding to alternative cations could produce alternative tertiary configurations. By utilizing circular dichroism and mobility gel-shift assays it was observed that tRNA structure can be altered when in the presence of different divalent cationic species. Formation of these alternative structural configurations was further validated by aminoacylating these tRNA structural anomalies with their native enzyme, which resulted in markedly different degrees of activity. Thus, it was confirmed that structural changes do occur when tRNA forms complexes with different cations. To better understand these structural changes, quantitative cation binding to tRNA was determined through titrations as well as ICP-OES analysis, which indicated that the metal ions can bind to the tRNA structure in specific and non-specific ways. Lastly, it was observed through stopped-flow kinetics that tRNA can associate/dissociate from different cations to varying degrees, thus forming cation-specific complexes at unique rates.
Contributors: Adrian Haas, Stephan Pfister, Christopher Zimdars
... This data is the result of the work and method developed in the scientific publication: "ENHANCING COMPREHENSIVE MEASUREMENT OF SOCIAL IMPACTS IN S-LCA BY INCLUDING ENVIRONMENTAL AND ECONOMIC ASPECTS", published in The International Journal of Life Cycle Assessment under DOI: 10.1007/s11367-017-1305-z It contains the limited version without the social hotspot database (SHDB). if you want to use the full version you need to have a license of SHDB and you can contact Stephan Pfister (email@example.com) to get further information on this. The data contains the MRIO data from EXIOBASE v2 and is only allowed to use with their data license: http://www.exiobase.eu/index.php/terms-of-use In summary, you need to pay a fee for commercial uses.
Exploiting Interactions of Heart-leaved Moon Seed, Tinospora cordifolia and yeast S. ceresiviae to Make Functional Herbal Wine
Contributors: Phyoe W. T.
... Plant products are widely used as functional foods all over the world for the quality, safety and efficiencies of their bioactive molecules. This study aims to improve the Myanmar folk medicine to the functional beverage and examine the functional properties of this beverage. Anaerobic fermentation of herbal wine was carried out for two months using S. ceresiviae. This herbal wine showed the 92% of antioxidant activity by DPPH free radical scavenging assay when compared to the reference compound ascorbic acid (250µg/ml). It has normoglycaemic Activity and can reduce the blood glucose level to the normal value after 135min of wine administration and thereafter maintained a plateau to the end of the study (225 min) in diabetic mice. Moreover, herbal wine showed antimicrobial activity on Pseudomonus sp. but not other tested bacteria by well diffusion assay. These in vitro and in vivo studies showed that this herbal wine have functional properties and have the potential for the treatment of type II diabetes in human by regular consummation.
Contributors: Agnel Sfeir, Aaron Phillips, Marco tigano, erika brunet
... Data included in Phillips et al.,