Data for: A comparison of transport methods for the solution of a problem with shadowing effects in spherical geometry
Contributors: Roberto Garcia
... Figures for the manuscript
Contributors: qiang LIU
... Polyurea nanocomposites have attracted increasing attention. In this article, in order to study the thermal properties and mechanical properties of polyurea composites, polyurea nanoparticles with different content of silicon carbide (SiC) nanoparticles (0.7wt%, 1.5wt% and 2.5wt%) were prepared via in-situ polymerization method. Using XRD,FTIR, SEM, TGA and tensile testing machine to characterize and analyze the surface morphology, structure, thermal and mechanical properties of polyurea nanocomposites. The results obtained from this study showed that the thermal stability and mechanical properties of polyurea composites as compared to pure polyurea can be improved by adding nano-SiC.
TIRF Microscopy Image Sequences of Fluorescent IgE-FcεRI inside a FcεRI-Centric Synapse in RBL-2H3 Cells Dataset
Contributors: Kathrin Spendier, Rachel Drawbond
... Total internal reflection fluorescence (TIRF) microscopy was used to image the IgE-FcεRI receptor signaling complex in rat basophilic leukemia (RBL-2H3) cells coming into contact with a supported lipid bilayer, modeling an immunological model synapse. Fluorescent immunoglobulin E (IgE488) concentrations ranged from 10% to 100%, in 10% increments and IgEdark that decreased from 90% to 0% by increments of 10%. To label, the IgE488 and IgEdark were added to the RBL-2H3 cells in suspension the day prior to imaging and allowed to incubate overnight. Immediately before experimental data was taken, cells were removed from the suspension dish and resuspended in Hank’s buffer. Cells of Sample 1 were kept in Hank’s buffer for a few minutes before cells were added to the SLB. Cells from Sample 2 were kept in Hank’s buffer for about half an hour before cells were added to the SLB. Cells from Sample 3 were kept in Hank’s buffer for about one hour before cells were added to the SLB. Multiple image sequenceswere taken for each of these ten conditions with camera exposure of 5 ms per image and pixel size of 107nm. The fluorescent labeling efficiency of IgE488 was 1.02 ± 0.08 mole fluorescent dye per mole protein. The dataset contains corresponding dark images and TIRF illumination profile images. The camera gain g was 0.006 photo electrons/ADU and the readout noise Nread was 0.529 photo electrons. The data are provided as OME-TIFF (.ome.tif), a life sciences file format. Information about the OME-TIFF (.ome.tif) file format information as well as imaging software supporting it can be found here: https://docs.openmicroscopy.org/ome-model/5.6.3/ome-tiff/
Contributors: Bingbing Wan
... These are the original gel images for MOLECULAR-CELLD-19-00528 paper
Contributors: Petra Bolechova, Michal Hradec, Helena Chaloupkova, Jana Doležalová, Eva Jánová
... Gibbons of the genus Nomascus exhibit strong sexual dichromatism in fur color. Change of fur color in sub-adult wild Nomascus females is associated with the onset of puberty and the time of their dispersing. The fact about the more observed reproductive suppression in monogamous species is known, particularly in females. The variability in color change may be therefore influenced by social factors. In this study, we determined whether captive Nomascus females establish reproductive maturity prior to dispersing with an association to their fur color. We collected 287 fecal extracts to analyze pregnandiol -3- glucuronide and 17β estradiol profiles of 4 sub-adult females (Nomascus leucogenys and Nomascus gabriellae) and 183 samples from their mothers, using enzyme immunoassays. The sub-adult females were monitored aging from 4 years old. Their hormone profiles showed the onset of ovulatory cycling between ages of 4.6-5.8 years. Based on the information about the estrogen influence to the secondary sex characteristic (color of female) the positive link between estrogen concentration and age of the sub-adult females was found. However, the amount of the estrogen can apparently determinate the presence of the mother. If the mother was presented, the level of estrogen was higher than if the mother was missing. Our findings suggest that the probability to the beige color of the sub-adult females increased with increased age and if they were without mother. Due to a small number of females in this study, we can only assume a certain degree of maternal influence to the fur color change of their daughters.
Data for: Increasing the incorporation of recycled PET on polymeric blends through the reinforcement with commercial nanoclays
Contributors: Carol Lopez de Dicastillo
... The effect of increasing post-consumer bottle recycled poly (ethylene terephthalate) (RPET) on optical, thermal (DSC and TGA) and mechanical properties of films prepared by extrusion blending of RPET and virgin PET (vPET) was investigated. Furthermore, morphology (XRD and TEM) and physical properties of vPET/RPET nanocomposites prepared with commercial organoclays were studied. Calorimetric and mechanical parameters of the vPET/RPET films without clay were mostly changed at concentrations greater than 80% of RPET. On the other hand, nanocomposites showed a combined intercalated/tactoid morphology. Higher percentages of short RPET chains favored polymer intercalation, increased elastic modulus, and produced a smaller impact on opacity in nanocomposites. Tensile strength was decreased by the formation of tactoid agglomerates. Ductility was increased by the occurrence of the stress oscillation phenomenon (SO) during the tensile test. SO was promoted with the increase of RPET concentration while the presence of clays inhibited it.
Contributors: Felipe Vargas Rios
... Data, figures and tables for "Sensitivity and specificity of timed Up & Go Test and falling risk in Chilean independent elderly"
Contributors: Timothy Omara, karungi shakilah
... Collection of water samples
Rearrangements within the U6 snRNA core during the transition between the two catalytic steps of splicing. Eysmont et al.
Contributors: Katarzyna Eysmont
... The RNA catalytic core of spliceosomes as visualized by cryo-EM remains unchanged at different stages of splicing. However, we demonstrate that mutations within the core of yeast U6 snRNA modulate conformational changes between the two catalytic steps. We propose that the intramolecular stem-loop (ISL) of U6 exists in two competing states, changing between a default, non-catalytic conformation and a transient, catalytic conformation. Whereas stable interactions in the catalytic triplex promote catalysis and their disruptions favor exit from the catalytic conformation, destabilization of the lower ISL stem promotes catalysis and its stabilization supports exit from the catalytic conformation. Thus, in addition to the catalytic triplex, U6-ISL acts as an important dynamic component of the catalytic center. The relative flexibility of the lower U6-ISL stem is conserved across eukaryotes. Similar features are found in U6atac and domain V of group II introns, arguing for the generality of the proposed mechanism.