Data for: Versatile use of Azospirillum brasilense strains tagged with gfp and mCherry genes for visualization of biofilm associated with wheat roots.
Contributors: Beatriz Baca, María Luisa Xiqui Vázquez, Alberto Ramirez , Miguel Ángel Ramales Pacheco, Saúl Jijón Moreno
... This study reports the introduction of egfp or mCherry markers to the Sp245, Sp7, and M40 wild-type strains of Azospirillum brasilense and the hhkB (encoding for a putative hybrid histidine kinase) minus mutant an isogenic strain of A. brasilense Sp245 to monitor colonization of wheat (Triticum aestivum). Two plasmids were constructed: (1) the pJMS-2 suicide plasmid derived from pSUP202 and harboring the mCherry gene expressed under the constitutive kanamycin resistance promoter to create a cis tag and (2) the broad-range plasmid pMP2449-5 that carries the mCherry gene under the lac promoter, which is derived from the plasmid pMP2444; to create the in trans tag. The stability of the plasmids encoding egfp and mCherry were confirmed in vitro for seven days of bacterial growth, and then, the A. brasilense strains harboring the plasmids were studied under nonselective conditions for adherence to seeds and, at seven or 14 days post-inoculation, for wheat root colonization. The utility of the labeled strains was proven by observation, using fluorescence microscopy and confocal laser scanning microscopy (CLSM) in wheat plants inoculated with the labeled strains and compared with the CFU g–1 for seed and wheat root. The method was suitable for observation of the in situ formation of mini-colonies, enabled visualization of bacterial colonization sites on large root fragments, and showed adherence to germinated seeds and root colonization of all strains by cell counts and direct microscopic examination. Thus, we are able to quantify the structures of the biofilms formed by each strain.
Contributors: Phil Prangnell
... Automated image analysis measurements and final selected images for paper
Contributors: Santiago Emilio Díaz Azpíroz
... These data speak about my concept of architecture, the relationships instead of people through public space to show their idiosyncracy or identity as a community
Data from Babin, Piganeau et al. "Chromosomal translocation formation is sufficient to produce fusion circular RNAs specific to patient tumor cells"
Contributors: ERIKA BRUNET, Loélia BABIN, Marion Piganeau, Thomas Mercher, ludovic deriano, Carine Giovannangeli, Cécile Thirant, Khadija Lamribet, Benjamin Renouf
... These data are linked to our paper "Chromosomal translocation formation is sufficient to produce fusion circular RNAs specific to patient tumor cells" from Babin et al; iScience 2018 Each figure raw data sets are available.
Contributors: Guangyu Wang
... All data used in the paper except for the original .oir images due to the large size of the files. The pptx. contains all the data in the figures which can be opened with GraghPad Prism.
Contributors: aswin Sundarakrishnan
... The data associated with this manuscript is available here.
Data for: Acute upper gastrointestinal hemorrhage from a pseudoaneurysm of an unusual superior polar artery of the spleen.
Contributors: Francisco Grippi, Hyeon Yu
... Abstract: Splenic artery aneurysm and pseudoaneurysm are rare pathologies and uncommon causes of massive upper gastrointestinal bleeding. They represent the third most common intra-abdominal aneurysms. Variations in the origin of splenic artery are relatively common and asymptomatic. However, the presence of an accessory splenic artery that is symptomatic is quite atypical. In this report, we describe the case of a 73-year-old female who presented with massive upper gastrointestinal bleeding caused by a pseudoaneurysm of a superior polar artery with an unusual anatomical origin. The patient was successfully treated endovascularly with transarterial coil embolization. Early diagnosis and interventional management is crucial for patient’s survival; hence, it must be kept in mind as possible etiology of life-threatening gastrointestinal bleeding to reduce morbidity and mortality.
Contributors: Ping He, Libo Shan, Pierce Jamieson
... Supplemental data delineating the identifiers of each of the genes referenced in the phylogenetic tree figure.
Data for: Facile green synthesis of carbon dots from Pyrus pyrifolia fruit for assaying of Al3+ ion via chelation enhanced fluorescence mechanism
Contributors: Suresh Kumar Kailasa, Jigna Bhamore, Sanjay Jha, Tae Jung Park, Rakesh Singhal
... Supporting Information of Facile green synthesis of carbon dots from Pyrus pyrifolia fruit for assaying of Al3+ ion via chelation enhanced fluorescence mechanism
Contributors: JUN ZHAO, Hong-ying Liu, Hong-tao Chang, Lu Chen, Chuan-qing Wang, Hong-jie Li, Yong-tao Li, Dong-sheng Gao, Yong-sheng Wang
... The data set provided the cytotoxic effect of LiCl treatment on Vero cells, inhibitory effects of LiCl on PEDV infection, inhibitory effects of LiCl on different stages of PEDV infection, and the effect of LiCl on cell apoptosis analyzed by fluorescence staining.