Contributors: Webber JL, Zhang J, Cote L, Vivekanand P, Ni X, Zhou J, N�gre N, Carthew RW, White KP, Rebay I

Date: 2013-03-01

ChIP-Seq of Yan protein in stage 11 Drosophila embryos ... High-resolution genome-wide binding of Yan used to confirm the presence of high-density regions seen in ChIP-chip ChIP-Seq of Yan protein in stage 11 Drosophila embryos

Contributors: Oscar Reina Garcia, Albert Carbonell, Salvador Pérez-Montero, Paula Climent-Cantó, Oscar Reina, Fernando Azorín

Date: 2017-12-13

ChIP-Seq peak calling of dBigH1 against input sample in early embryos ...Drosophila melanogaster) Organism: Drosophila melanogaster Published...ChIP-Seq] ... Accession Number: GSE103291 Platform: GPL11203: Illumina Genome Analyzer IIx (Drosophila melanogaster) Organism: Drosophila melanogaster Published on 2017-12-13 Summary: This analysis largely reflected the genomic distribution of dBigH1 in 0-2hpf early embryos. In these studies, we observed that dBigH1 was uniformely distributed across the genome. Overall Design: ChIP-Seq peak calling of dBigH1 against input sample in early embryos Contact: Name: Oscar Reina Garcia Organization: IRB Barcelona Deparment: Biostatistics and Bioinformatics Address: C/Baldiri Reixac 10 Barcelona Barcelona 08028 Spain Email: oscar.reina@irbbarcelona.org Organization: GEO Address: USA

Contributors: Mattias Mannervik, Ann Boija, Per Stenberg

Date: 2015-11-06

ChIP seq in Drosophila S2 cells using two different antibodies against...Drosophila melanogaster) Organism: Drosophila melanogaster Published...ChIP-seq data from Drosophila S2 cells and compared it to modENCODE data...Drosophila S2 cells ... Accession Number: GSE64464 Platform: GPL14601: AB SOLiD 4 System (Drosophila melanogaster) Organism: Drosophila melanogaster Published on 2015-11-06 Summary: CREB-binding protein (CBP, also known as nejire) is a transcriptional co-activator that is conserved in metazoans. We have generated CBP ChIP-seq data from Drosophila S2 cells and compared it to modENCODE data. This shows that CBP is bound at genomic sites with a wide range of functions. As expected, we find that CBP is bound at active promoters and enhancers. In addition, we find that the strongest CBP sites in the genome are found at Polycomb Response Elements embedded in histone H3 lysine 27 trimethylated (H3K27me3) chromatin, where they correlate with binding of the Pho repressive complex. Interestingly, we find that CBP also binds to most insulators in the genome. At a subset of these, CBP may regulate insulating activity, measured as the ability to prevent repressive H3K27 methylation from spreading into adjacent chromatin. Overall Design: ChIP seq in Drosophila S2 cells using two different antibodies against CBP (nejire), one raised in rabbit against amino acids 2540-3190 (CBP rb), and one raised in guinea-pig against amino acids 1-178 (CBP gp) Contact: Name: Mattias Mannervik Organization: Stockholm University Laboratory: Mattias Mannervik Deparment: Molecular Biosciences, the Wenner-Gren Institute Address: Arrheniuslaboratories E3 Stockholm 10691 Sweden Email: mattias.mannervik@su.se Organization: GEO Address: USA

Contributors: Oscar Reina Garcia, Albert Carbonell, Salvador Pérez-Montero, Paula Climent-Cantó, Oscar Reina, Fernando Azorín

Date: 2017-12-13

ChIP-Seq peak calling of dBigH1 against input sample in whole testes ...Drosophila melanogaster) Organism: Drosophila melanogaster Published...ChIP-Seq] ... Accession Number: GSE96959 Platform: GPL11203: Illumina Genome Analyzer IIx (Drosophila melanogaster) Organism: Drosophila melanogaster Published on 2017-12-13 Summary: This analysis largely reflected the genomic distribution of dBigH1 in spermatocytes since the vast majority of dBigH1-expressing cells in testes correspond to spermatocytes. In these studies, we observed that dBigH1 was not uniformely distributed across the genome. Overall Design: ChIP-Seq peak calling of dBigH1 against input sample in whole testes Contact: Name: Oscar Reina Garcia Organization: IRB Barcelona Deparment: Biostatistics and Bioinformatics Address: C/Baldiri Reixac 10 Barcelona Barcelona 08028 Spain Email: oscar.reina@irbbarcelona.org Organization: GEO Address: USA

Contributors: Jian Zhou, Wangjie Yu, Paul E. Hardin

Date: 2015-01-01

Drosophila that can be used to assess protein–DNA-binding rhythms at specific...Drosophila Clock...ChIP-seq. ChIP analysis has revealed the relationship between clock factor...Drosophila...ChIP-seq). ChIP has been widely used in circadian biology to assess rhythmic ... In eukaryotes, the circadian clock controls 24h rhythms in physiology, metabolism, and behavior via cell autonomous transcriptional feedback loops. These feedback loops keep circadian time and control rhythmic outputs by driving rhythms in transcription; thus, it is important to determine when clock transcription factors bind their target sequences in vivo to promote or repress transcription. Interactions between proteins and DNA can be measured in cells, tissue, or whole organisms using a technique called chromatin immunoprecipitation (ChIP). The principle underlying ChIP is that protein is cross-linked to associated chromatin to form a protein–DNA complex, the DNA is then sheared, and the protein of interest is immunoprecipitated. The cross-links are then removed from the antibody–protein–DNA complex, and the associated DNA fragments are purified. The DNA is then used to quantify specific targets by real-time quantitative PCR or to generate libraries for global analysis of protein target sites by high-throughput sequencing (ChIP-seq). ChIP has been widely used in circadian biology to assess rhythmic binding of clock components, RNA polymerase II, and rhythms in chromatin modifications such as histone acetylation and methylation. Here, we present a detailed method for ChIP analysis in Drosophila that can be used to assess protein–DNA-binding rhythms at specific genomic target sites. With minor modifications, this technique can be used to assess protein–DNA-binding rhythms at all target sites via ChIP-seq. ChIP analysis has revealed the relationship between clock factor binding, transcription, and chromatin modifications and promises to reveal circadian transcription networks that control phase and tissue specificity.

Contributors: Suchit Jhunjhunwala, Brian Egan, Madeline Craske, Paul Labhart, Chih-Chi Yuan, Gulfem D Guler, David Arnott, Tobias Maille, Jennifer Busby, Chisato Henry

Date: 2016-01-31

Drosophila chromatin into human ChIP samples and added a Drosophila-specific...ChIP-seq spike-in method enables detection of global histone modification...Drosophila chromatin as a minor fraction of the total ChIP DNA. The Drosophila...ChIP-seq tags are used to normalize the human ChIP-seq data from DMSO ...ChIP-seq data and will improve the quantitative comparison of histone ... Accession Number: GSE64243 Platform: GPL16791: Illumina HiSeq 2500 (Homo sapiens) Organism: Homo sapiens Published on 2016-01-31 Summary: This study outlines a method that dramatically alters the interpretation of ChIP-seq data and will improve the quantitative comparison of histone modification maps across biological contexts or across various conditions within a given biological context. Overall Design: We introduced a small fraction of Drosophila chromatin into human ChIP samples and added a Drosophila-specific antibody as a means to consistently precipitate Drosophila chromatin as a minor fraction of the total ChIP DNA. The Drosophila ChIP-seq tags are used to normalize the human ChIP-seq data from DMSO and EZH2 inhibitor-treated samples. Employing this strategy, a substantial reduction in H3K27me3 levels is observed across the genome upon EZH2 inhibitor treatment. Contact: Name: Suchit Jhunjhunwala Organization: Genentech Address: 1 DNA Way, MS-93 South San Francisco CA 94080 USA Email: suchitj@gene.com Organization: GEO Address: USA

Contributors: DCC modENCODE, David MacAlpine, Heather MacAlpine, Matthew Eaton, Joseph Prinz

Date: 2012-05-29

Drosophila ORC2p); read length (read_length) Contact: Name: DCC modENCODE...ChIP-Seq experiment...Drosophila melanogaster) Organism: Drosophila melanogaster Published...CHIP-seq. BIOLOGICAL SOURCE: Developmental Stage: Embryo 0-2h; EXPERIMENTAL ... Accession Number: GSE38293 Platform: GPL9058: Illumina Genome Analyzer (Drosophila melanogaster) Organism: Drosophila melanogaster Published on 2012-05-29 Summary: modENCODE_submission_3628 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We will precisely identify sequence elements that direct DNA replication by using chromatin immunoprecipitation of known replication initiation complexes. These experiments will be conducted in multiple cell types and developmental tissues. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Overall Design: EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Developmental Stage: Embryo 0-2h; EXPERIMENTAL FACTORS: Developmental Stage Embryo 0-2h; Antibody dORC2 (target is Drosophila ORC2p); read length (read_length) Contact: Name: DCC modENCODE Organization: Ontario Institute for Cancer Research Laboratory: modENCODE DCC Address: MaRS Centre, South Tower, 101 College Street, Suite 800 Toronto Ontario Canada Email: help@modencode.org Phone: 416-673-8579 Organization: GEO Address: USA

Contributors: DCC modENCODE, David MacAlpine, Heather MacAlpine, Matthew Eaton, Joseph Prinz

Date: 2012-02-27

Drosophila ORC2p); Developmental Stage Embryo 4-7h Contact: Name: DCC...ChIP-Seq experiment...CHIP-seq. BIOLOGICAL SOURCE: Strain: Oregon-R(official name : Oregon-R-modENCODE...Drosophila melanogaster) Organism: Drosophila melanogaster Published ... Accession Number: GSE36106 Platform: GPL9058: Illumina Genome Analyzer (Drosophila melanogaster) Organism: Drosophila melanogaster Published on 2012-02-27 Summary: modENCODE_submission_4192 This submission comes from a modENCODE project of David MacAlpine. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: We will precisely identify sequence elements that direct DNA replication by using chromatin immunoprecipitation of known replication initiation complexes. These experiments will be conducted in multiple cell types and developmental tissues. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Overall Design: EXPERIMENT TYPE: CHIP-seq. BIOLOGICAL SOURCE: Strain: Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); Developmental Stage: Embryo 4-7h; Genotype: wild type; EXPERIMENTAL FACTORS: Strain Oregon-R(official name : Oregon-R-modENCODE genotype : wild type ); read length (read_length) ; Antibody dORC2 (target is Drosophila ORC2p); Developmental Stage Embryo 4-7h Contact: Name: DCC modENCODE Organization: Ontario Institute for Cancer Research Laboratory: modENCODE DCC Address: MaRS Centre, South Tower, 101 College Street, Suite 800 Toronto Ontario Canada Email: help@modencode.org Phone: 416-673-8579 Organization: GEO Address: USA

Contributors: Georgi K. Marinov, Jie Wang, Dominik Handler, Barbara J. Wold, Zhiping Weng, Gregory J. Hannon, Alexei A. Aravin, Phillip D. Zamore, Julius Brennecke, Katalin Fejes Toth

Date: 2015-03-23

ChIP-seq dataset and high enrichment of H3K9me3 (from Muerdter et al.,...ChIP-seq and background (input) data from Huang et al. showing (1) unique...Piwi ChIP-seq dataset. (B) Strong apparent enrichment over individual ...ChIP-seq enrichment observed over some individual transposable elements...ChIP-seq and input RPM levels over the transposon consensus sequences ...Drosophila. Their study also reported that loss of Piwi causes widespread...ChIP-seq data from Huang et al. (red) and H3K9me3 data from Muerdter et...ChIP-seq) reveals the genome-wide sites of occupancy by Piwi, a piRNA-guided ... Huang et al. (2013) recently reported that chromatin immunoprecipitation sequencing (ChIP-seq) reveals the genome-wide sites of occupancy by Piwi, a piRNA-guided Argonaute protein central to transposon silencing in Drosophila. Their study also reported that loss of Piwi causes widespread rewiring of transcriptional patterns, as evidenced by changes in RNA polymerase II occupancy across the genome. Here we reanalyze their data and report that the underlying deep-sequencing dataset does not support the authors’ genome-wide conclusions.

Contributors: David A. Orlando, Mei Wei Chen, Victoria E. Brown, Snehakumari Solanki, Yoon J. Choi, Eric R. Olson, Christian C. Fritz, James E. Bradner, Matthew G. Guenther

Date: 2014-01-01

orange). ChIP-seq signals were calculated based on traditional or Drosophila-reference-normalized... of ChIP-Seq Data (A) Schematic representation of a typical ChIP-seq data...ChIP-Seq Normalization Reveals Global Modulation of the Epigenome...ChIP-seq peaks (blue). A comparison of the peaks as a percentage of the...ChIP-seq in the presence of the reference Drosophila epigenome (orange...ChIP-Seq Data (A) Schematic representation of a typical ChIP-seq data ...ChIP-seq) is a prevailing methodology used to investigate chromatin-based...subjected to ChIP-seq in the presence of the reference Drosophila epigenome...reveals ChIP-seq peaks (blue). A comparison of the peaks as a percentage...ChIP-seq experiments enables the discovery of disease-relevant changes...ChIP-seq signals were calculated based on traditional or Drosophila-reference-normalized...ChIP-seq reads aligning to either test (human, blue) or Drosophila (reference ... Epigenomic profiling by chromatin immunoprecipitation coupled with massively parallel DNA sequencing (ChIP-seq) is a prevailing methodology used to investigate chromatin-based regulation in biological systems such as human disease, but the lack of an empirical methodology to enable normalization among experiments has limited the precision and usefulness of this technique. Here, we describe a method called ChIP with reference exogenous genome (ChIP-Rx) that allows one to perform genome-wide quantitative comparisons of histone modification status across cell populations using defined quantities of a reference epigenome. ChIP-Rx enables the discovery and quantification of dynamic epigenomic profiles across mammalian cells that would otherwise remain hidden using traditional normalization methods. We demonstrate the utility of this method for measuring epigenomic changes following chemical perturbations and show how reference normalization of ChIP-seq experiments enables the discovery of disease-relevant changes in histone modification occupancy.