Mendeley Data Showcase

This dataset contains all raw and processed data supporting the findings of the research article titled "Harpagide from Scrophularia Alleviates Cerebral Ischemic Injury via AMPK-MCU Phosphorylation-Dependent Inhibition of Mitochondrial Calcium Overload“. The data provided herein enable the full replication of the statistical analyses, figures, and conclusions presented in the manuscript. The study investigates the neuroprotective mechanism of harpagide against cerebral ischemia/reperfusion injury, focusing on the AMPK-MCU mitochondrial calcium homeostasis axis. Data were generated from both in vitro oxygen-glucose deprivation (OGD) models using PC12 and SH-SY5Y cell lines and an in vivo mouse model of transient middle cerebral artery occlusion (MCAO). The dataset comprises the following main categories: In vitro Pharmacological and Biochemical Data (PC12 & SH-SY5Y cells): Cell Viability & Apoptosis: Raw flow cytometry data (Annexin V-FITC/PI staining) for apoptosis rates across experimental groups (Control, OGD, OGD+Harpagide, OGD+RR, OGD+Compound C). Cell Injury and Oxidative Stress Markers: Raw absorbance/fluorescence values for Lactate Dehydrogenase (LDH) release, Malondialdehyde (MDA) content, Superoxide Dismutase (SOD) activity, and intracellular ATP levels. Mitochondrial Function Parameters: Raw fluorescence intensity data for Reactive Oxygen Species (ROS) detection (DCFH-DA probe), mitochondrial membrane potential (JC-1 probe), and mitochondrial calcium concentration ([Ca2+]mito, Rhod-2 AM probe). Gene and Protein Expression:Raw Cycle Threshold (Ct) values from RT-qPCR for MCU, AIF, Endo G, Cyt C, Caspase-3， and AMPK mRNA /protein expression. In vivo Neuroprotective Efficacy Data (MCAO Mice): Neurological and Infarct Assessment: Individual animal scores from the Longa neurological deficit scale. Raw image files and subsequent calculated infarct volume percentages from Triphenyltetrazolium Chloride (TTC) staining. Cerebral Blood Flow (CBF): Raw perfusion unit data or derived relative CBF values from Laser Speckle Contrast Imaging (LSCI) for each mouse. Mitochondrial Ultrastructure: Representative transmission electron microscopy (TEM) images of cortical neuron mitochondria from different treatment groups. Tissue Protein Expression: Raw data from immunohistochemical (IHC) analysis quantifying the MCU-positive expression area in the temporal cortex. Acknowledgements and Citation This work was supported by grants from the Natural Science Key Foundation of Zhejiang, China (No. LZ25H310002), National Natural Science Foundation of China (No.82204837,No.82073824), Natural Science Foundation of Zhejiang, China (No. LQ23H290004), Jiaxing Key Laboratory of Brain Protection Basic and Clinical Research in Acute Ischemic cerebrovascular Disease. If you use this dataset in your research, please cite both the associated research article (when available) and this dataset using its persistent DOI provided by the repository

This dataset contains UV absorbance values measured between 290 and 320 nm for ethanolic extracts of five freshwater microalgae species. The absorbance data were generated to evaluate the in vitro sun protection factor (SPF) of the extracts using the Mansur spectrophotometric method. The dataset supports the assessment of the photoprotective potential of freshwater microalgae as natural sources of UV-protective compounds.

Data and processing related to the article entitled “Dynamics of assortments under different treatments of early thinning from above in Eucalyptus dunnii Maiden”

The attached csv files contain the results to the synthetic testbed for disaster variants: PO-B, PA-L, and PA-B. These are in reference to the paper: Optimizing Resilient Facility Locations under Natural Disaster Disruptions.

This dataset contains the original, uncropped western blot images supporting all figures in the manuscript titled ‘[Porcine IRF2 suppresses the cGAS-STING mediated antiviral IFN signaling by competing IRF3 for binding to the IFN promoter]’. For each figure panel, the corresponding raw files are provided.

Objective: Healthcare providers perform approximately 300 million major non-cardiac surgeries worldwide yearly and individualized risk assessments can significantly reduce perioperative complications. Telemedicine improves access to specialists, but its effectiveness in these evaluations is not fully understood. This study aims to investigate the clinical characteristics and management strategies of patients referred for telecardiology consultations specifically for preoperative cardiovascular risk assessment. Methods: A retrospective observational study at a Telemedicine Center in São Paulo, Brazil, reference for 380 primary health care facilities was performed from May 2021 to March 2024. We included adults over 18 years old. Thirty-five cardiologists conducted real-time teleconsultations alongside general practitioners, reviewing patient data, surgical type, clinical details, complementary exams, and consultation outcomes. Descriptive statistics were used for analysis. Results: A total of 3,476 patients (63% female, mean age 52.1 years) were evaluated. Among them, 3,307 (95.1%) were asymptomatic, 1,241 (35.70%) had hypertension, and 1,312 (48.4%) led sedentary lifestyles. The most frequently performed surgical procedure was intra-abdominal surgery, performed in 1,665 (47.9%) cases. Risk stratification categorized 3,161 (90.93%) patients as low risk, 90 (2.59%) as intermediate risk, and 36 (1.03%) as high risk. Ancillary testing was requested in 504 (14.50%) cases, with laboratory evaluations conducted in 304 (8.74%) and electrocardiograms in 234 (6.73%). Telecardiology interventions encompassed antihypertensive adjustments for 192 (5.52%) patients, statin prescriptions for 88 (2.53%), and guidance for postoperative monitoring in 98 (2.81%). Conclusion: This exploratory study demonstrates that most cardiologists successfully perform perioperative risk stratification via telemedicine during the initial consultation, targeting a predominantly low-risk population.

Trata-se de um estudo transversal descritivo, que analisou as notificações de anomalias congênitas no Sistema de Informações sobre Nascidos Vivos e os atributos qualidade dos dados (completude e consistência), simplicidade, flexibilidade, representatividade e oportunidade conforme, diretrizes do Centers for Disease Control and Prevention dos Estados Unidos.

The R scripts included in this dataset perform the following tasks: • Data cleaning and merging across multiple NHANES cycles • Construction of nighttime energy and macronutrient variables • Categorization of nighttime intake levels (e.g., none, moderate, high) • Survey-weighted logistic regression models • Subgroup and sensitivity analyses • Data-driven clustering of nighttime macronutrient composition patterns • Visualization of results, including forest plots and pattern comparisons

Original data of fig 3,4,5 and Table 4,5,6

Replication data and code for Floods and Urban Density