MIMBLE 1

Published: 15-04-2021| Version 2 | DOI: 10.17632/2w9kjrb682.2
Contributors:
Nuala Calder,
Kevin Walsh,
Peter Olupot Olupot,
Tonny Ssenyondo,
Rita Muhindo,
Ayub Mpoya,
Jerusa Brignardello,
Xuedan Wang,
Eleanor McKay,
Douglas Morrison,
Elaine Holmes,
Gary Frost,
Kathryn Maitland

Description

Outcomes in African children hospitalised with severe acute malnutrition (SAM) remain poor, and gastrointestinal function may be altered. A 3-arm pilot trial in 58 Ugandan children hospitalised with SAM compared feeds enriched with cowpea (CpF, n=20) or inulin (InF, n=20), and conventional feeds (ConF, n=18) investigating their safety, effectiveness and effects on gut function to 28 days. No differences were observed in proportion achieving weight gain >5g/kg/day (87%, 92%, 86%, p>0.05), 28-day mortality (16%, 30%, 17% p>0.05), or proportion achieving oedema resolution (83%, 54%, 91% p>0.05) between CpF, InF and ConF. Baseline measurements of gut permeability (lactulose:mannitol ratio mean 1.19 ± SD 2.00), inflammation (faecal calprotectin median 539.0ug/g stool IQR 904.8) and satiety (plasma polypeptide YY median 62.6pmol/l IQR 110.3) confirmed gastrointestinal dysfunction. Decrease in faecal bacterial richness from day 1 (ACE = 53.2) to day 7 (ACE = 40.8) was observed only in ConF (p=0.025). Bifidobacterium increased from day 7 (5.8% ± 8.6) to day 28 (10.9% ± 8.7) in CpF but not significantly (p=0.78). Data included in this upload: - Metadata - Faecal short chain fatty acid concentration (GC-MS) - Urine and plasma NMR chemical shift - Plasma PYY and GLP-1 - Faecal 16S rRNA sequencing OTU/Taxonomy - Faecal bacterial richness and diversity statistics

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