Data for: Mechanism of nitric oxide and acid-sensing ion channel 1a modulation of panic-like behaviour in the dorsal periaqueductal grey
Description
Fig. 1. Photograph of the study apparatus. Fig. 2. Effects of SNAP infusion (10 or 20 nmol/0.2 μl) in the dPAG on ethological and spatiotemporal measures. (A) Freezing time; (B) stretched-attend posture (SAP); (C) time in contact with the grid wall; and (D) time in the protected area. Significant differences between the treatment and control groups were determined via one-way ANOVA and Dunnett’s multiple comparison procedure (* P < 0.05, ** P < 0.01, and *** P < 0.001 compared with the control group injected with vehicle. n = 8-10 mice/group). Fig. 3. Effects of L-NAME infusion (100 or 300 nmol/0.2 μl) in the dPAG on ethological and spatiotemporal measures. (A) Freezing time; (B) stretched-attend posture (SAP); (C) time in contact with the grid wall; and (D) time in the protected area. Significant differences between the treatment and control groups were determined via one-way ANOVA and Dunnett’s multiple comparison procedure (** P < 0.01 and *** P < 0.001 compared with the control group injected with vehicle. n = 8-10 mice/group). Fig. 4. Effects of PcTx-1 infusion (20 or 50 ng/0.2 μl) in the dPAG on ethological and spatiotemporal measures. (A) Freezing time; (B) stretched-attend posture (SAP); (C) time in contact with the grid wall; and (D) time in the protected area. Significant differences between the treatment and control groups were determined via one-way ANOVA and Dunnett’s multiple comparison procedure (* P < 0.05 and ** P < 0.01 compared with the control group injected with vehicle. n = 8-10 mice/group). Fig. 5. Effects of SNAP treatment on ASIC1a, p-CaMKIIα, and CaM expression levels in the dPAG. Representative Western blot image (A) and results of quantification of the expression levels of ASIC1a (B), p-CaMKIIα (C) and CaM (D) in the vehicle control (0) and SNAP treatment groups. Significant differences between the treatment and control groups were determined via one-way ANOVA and Dunnett’s multiple comparison procedure (*P < 0.05 and **P < 0.01 compared with the control group). Fig. 6. Effects of L-NAME treatment on ASIC1a, p-CaMKIIα, and CaM expression levels in the dPAG. Representative Western blot image (A) and results of quantification of the expression levels of ASIC1a (B), p-CaMKIIα (C) and CaM (D) in the vehicle control (0) and SNAP treatment groups. Significant differences between the treatment and control groups were determined via one-way ANOVA and Dunnett’s multiple comparison procedure (*P < 0.05 and **P < 0.01 compared with the control group). Fig. 7. Effects of PcTx-1 treatment on p-CaMKIIα and CaM expression levels in the dPAG. Representative Western blot image (A) and analysis to quantify the expression levels of p-CaMKIIα (B) and CaM (C) in the vehicle control (0) and SNAP treatment groups. Significant differences between the treatment and control groups were determined via one-way ANOVA and Dunnett’s multiple comparison procedure (*P < 0.05 compared with the control group).