Szojka et al_PLOS ONE 2020 Submission

Published: 22 March 2021| Version 2 | DOI: 10.17632/4ttbwnsf6z.2
Adetola Adesida




Steps to reproduce

Cell counting - haemocytometer and light microscopy 3D Cell culture on scaffolds - type I collagen scaffold Biochemical assays - GAG (on V-max kinetic microplate reader by Molecular Devices) and DNA ( via CYQUANT Cell Proliferation Assay Kit (ThermoFisher) on CytoFluor II fluorescence multi-well plate reader (PerSeptive Biosystems) measurements. Quantitative gene expression - SYBR Green detection (Eurogentec) on a CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories). Mechanical testing - on TA Instruments Biodynamic 5210 System