Maternal prenatal restraint stress effects on immune factors in mice

Published: 11 April 2022| Version 2 | DOI: 10.17632/5tfj857sb2.2


Maternal immune dysregulation, caused by gestational psychological stress, infection, and other perturbations, results in altered offspring neurodevelopment and psychiatric risk. Multiple immune factors (e.g., cytokines, chemokines) play critical roles in shaping offspring neurodevelopment after gestational stress, though how maternal stress impacts maternal, placental, and fetal cytokines more broadly remains unclear. This dataset contains detection and concentration data on IL-1β, IL-2, IL-4, IL-6, IL-10, IL-17A, IFNγ, and TNFα levels in maternal serum, fetal brain, and placenta after exposure to a widely-used mouse prenatal restraint stress manipulation. This study assessed cytokine alterations in offspring tissues and maternal serum due to maternal restraint stress. Two cohorts of CD-1 mice were time-mated and given repetitive restraint stress on gestational days 12-14. Prenatal stress dams were restrained in a plexiglass tube under bright lights for 45-min sessions during the light cycle (three times at E12 and E13 and once approximately 30 min before collection on E14). Dams were euthanized and tissues collected on embryonic day 14. Combined across the two cohorts, tissues from a total of 8 non-stressed and 9 stressed litters were collected. The following samples were taken: maternal serum, one randomly selected placenta per litter, and microdissected dorsal forebrain. Two Bio-Plex Mouse Cytokine Group I 8-plex Assays were used to assess levels of IL-1β, IL-2, IL-4, IL-6, IL-10, IL-17A, IFNγ, and TNFα. All samples were run in duplicate on a Bio-Plex Luminex 200 (Bio-Rad) and analyzed via Bio-Plex Manager (Bio-Rad), per manufacturer recommendations. Tissue analyte levels were normalized to total protein levels (Pierce BCA Protein Assay, Thermo Fisher). All analytes were not detected in all tissues. All experimental procedures involving animals were performed in accordance with University of Iowa Institutional Animal Care and Use Committee (IACUC) policies and NIH guide for the care and use of laboratory animals.



University of Iowa


Cytokines, Prenatal Stress