Alignment of fiducial marks in a tomographic tilt series with an unknown rotation axis

Published: 01-06-2007| Version 1 | DOI: 10.17632/8vnypzjs58.1
Zachary H. Levine,
Alex Volkovitsky,
Howard K. Hung


Abstract Alignment for tomography using a transmission electron microscopy frequently uses colloidal gold particles as fiducial reference marks. Typically, there is an implicit assumption that the tilt axis of the tomographic series is orthogonal to the beam direction. However, this may not be true, either intentionally, if a tilt-rotate stage is used, or unintentionally, because of mechanical errors in the rotation stage or the sample fixture. Here, we provide a computer code which takes as input a s... Title of program: particleTilt Catalogue Id: ADYW_v1_0 Nature of problem The program is used to assist the alignment step in tomography. The samples should be prepared with spherical particles (typically gold beads) which are observed in several views. (Not every particle need be observed in every view.) The program reports coordinates of a 3D model of the particles as well as the direction of the tilt axis as a point on the unit sphere. Versions of this program held in the CPC repository in Mendeley Data ADYW_v1_0; particleTilt; 10.1016/j.cpc.2007.01.008 This program has been imported from the CPC Program Library held at Queen's University Belfast (1969-2019)