Hodel et al Original Western blot data
Original Western blot data used for Figure S3 in Hodel et al. 50 ug of cell lysate from the indicated cell lines were separated via SDS-PAGE and transferred to nitrocellulose membrane using standard conditions. The blot was then physically cut into sections and stained with the indicated primary antibodies, inlcuding p53, P-p53, Mdm2, p21, PUMA and GAPDH as a loading control. Longer exposures are also provided for Mdm2 and p53.