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Theriogenology

ISSN: 0093-691X

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Datasets associated with articles published in Theriogenology

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1970
2024
1970 2024
3 results
  • Data for: SPERM MOTILITY AND LIPID COMPOSITION IN INTERNALLY FERTILIZING OCELLATE RIVER STINGRAY POTAMOTRYGON MOTORO
    File with CASA data of stingray sperm motility
    • Dataset
  • Data for: Menstrual cycle in four New World primates: Poeppig’s woolly monkey (Lagothrix poeppigii), red uakari (Cacajao calvus), large-headed capuchin (Sapajus macrocephalus) and nocturnal monkey (Aotus nancymaae)
    Genital organs from 33 Aotus namcymaae, 29 Poeppig’s woolly monkeys (Lagothrix poepigii), 21 red uakaris (Cacajao calvus) and 11 large-headed capuchins (Sapajus macrocephalus) were histologically analyzed in order to describe the endometrial changes related to the ovarian cycle. Recorded ovarian parameter included number and diameter of the largest antral follicle and CL. Recorded uterine variables include the density of endometrial glands, hemosiderin and fibrin measured and ranked from 0 to 5, according to the average number of target structures counted in random 1-mm2 fields at 100X.
    • Dataset
  • Dataset for Evaluation of a novel microfluidic chip-like device for purifying bovine frozen-thawed semen for in vitro fertilization
    VetCountTM Harvester (MotilityCount ApS, Copenhagen, Denmark) is a novel sperm purification device. It consists of two chambers separated by a 10 μM microporous membrane. Untreated semen is applied in one chamber, sperm collection medium in the other. Motile sperm cells are selected by actively swimming through the membrane pores into the medium containing chamber. After 30 min incubation, the sperm collection medium can be aspirated and the purified sperm is ready for further use. In a first experiment we assessed sperm quality and recovery of frozen-thawed semen from six different bulls (n = 6) prior to and after purification with the VetCountTM Harvester or BoviPureTM gradient centrifugation, a commercial available standard technique. In a second approach, a competitive fertilization assay was performed. Ten straws per bull were pooled, split in two subsamples, and simultaneously purified either with the VetCountTM Harvester or BoviPureTM gradient centrifugation. Following purification, sperm cells from each treatment group were fluorescently labeled with either MitoTrackerTM Red FM or MitoTrackerTM Green FM. In vitro matured oocytes were inseminated with equal numbers of red and green labeled sperm. Eighteen hours after fertilization, fluorescence microscopy was used to determine the origin of the fertilizing spermatozoon.
    • Dataset