Journal of Microbiological Methods

The template and taxonomic files of nxrB gene of Nitrospira for processing the high throughput sequencing data with the MOTHUR

Number of true positive and negative and false positive and negative for each study used in the meta-analysis. Some information about the use of unformed stools and patient's age is also provided.

Flow cytometry data on gene expression dynamics of RNA and corresponding proteins

Spectra generated using the Absciex TOF/TOF 5800 MALDI instrument with results from MABRITEC

The sequencing data obtained from Ion Reporter program for 16S hypervariable regions V3 and V6 for different treatments.

Supplementary Materials: Figure S1: HPLC analysis of astaxanthin, Table S1: Plasmids used in this study, Table S2: Primers used for qRT-PCR analysis. Table S3: A residual endotoxin detection of FFAX.

Concentration and degree of purity of DNA extracted by method.

The uploaded file includes part of raw data obtained during experimental work. The another part of data could not be uploaded due to confidential status (future usage).

This data set is for a submission of a manuscript to a peer-reviewed journal. A Rapid Viability Polymerase Chain Reaction (RV-PCR) method for detection of Yersinia pestis in water samples was developed under a research project. An final internal report for this work was cleared in the STICS in 2016. This manuscript contains a subset of data from the final report.

This is a short communication manuscript entitled, “Rapid Viability (RV) PCR Protocol for Detection of Francisella tularensis” to be published in a peer-reviewed journal. This pathogen survives for many days in the environment and water. It is a very slow growing pathogen and can take several days to detect using the traditional microbiological culture methods. Therefore, a high-throughput RV-PCR protocol was developed and optimized by the EPA ORD’s National Homeland Security Research Center (NHSRC) to relatively rapidly detect F. tularensis. The RV-PCR method combines relatively shorter sample enrichment in growth media, and highly specific and sensitive PCR assay-based detection and identification of F. tularensis. Using this method, results can be obtained in 36 hours as compared to several days by the traditional microbiological culture methods. This manuscript contains summarized data from multiple experimental results to show the method performance in various experimental conditions mimicking a real-world interference material. The data has been extracted from the final report for the project on Optimization of a Rapid Viability (RV) PCR Protocol for Detection of Francisella tularensis in water.