Brain Behavior and Immunity

Mesoscale 29 VPlex Mouse cytokine array performed on plasma of mice run through 10-day social defeat paradigm (RSDS) or controls. Social interaction test and elevated zero maze parameters also provided.

RNA sequencing data (raw counts and TPM) of monocyte-derived microglia-like cells, monocytes and primary microglia

The physiological and motivational effects of heroin and other abused drugs become associated with environmental (contextual) stimuli during repeated drug use. As a result, these contextual stimuli gain the ability to elicit drug-like conditioned effects. For example, after context-heroin pairings, exposure to the heroin-paired context alone produces similar effects on peripheral immune function as heroin itself. Conditioned immune effects can significantly exacerbate the adverse health consequences of heroin use. Our laboratory has shown that exposure to a heroin-paired context suppresses lipopolysaccharide (LPS)-induced splenic nitric oxide (NO) production in male rats, and this effect is mediated in part by the dorsal hippocampus (dHpc). However, specific dHpc output regions, whose efferents might mediate conditioned immune effects, have not been identified, nor has the contribution of ventral hippocampus (vHpc) been investigated. Here, we evaluated the role of CaMKIIα-expressing neurons in the dHpc and vHpc main output regions by expressing Gi-coupled designer receptors exclusively activated by designer drugs (DREADDs) under a CaMKIIα promoter in the dorsal subiculum and CA1 (dSub, dCA1) or ventral subiculum and CA1 (vSub, vCA1). After context-heroin conditioning, clozapine-N-oxide (CNO, DREADD agonist) or vehicle was administered systemically prior to heroin-paired context (or home-cage control) exposure and LPS immune challenge. Chemogenetic inhibition of CaMKIIα-expressing neurons in dHpc, but not vHpc, output regions attenuated the expression of conditioned splenic NO suppression. These results establish that the main dHpc output regions, the dSub and dCA1, are critical for this context-heroin conditioned immune effect. This is the raw data from nitrate assays, ELISAs, and qPCR for iNOS and nitric oxide for each experiment.

Genome-wide gene expression data for the experimental conditions outlined in the accompanying paper

Data for Fold changes in Moodinflame dataset for the three sites. Group effects were assessed with variable "group" "suicide_risk" and "agecat2". Sex was mistakenly named "Gender" but refers to biological sex. This was not changed in this repository to keep the compatibility with the original version.

The R data file (supp.RData) contains a list object that has observed covariance matrixes, residual correlation matrixes, approximate fit indices, and weighted least squares diagonal weight matrixes (models 2-3 only) for the three primary models. The .R file (supp.R) demonstrates how to access the data in the supp.RData file.

Data used for the manuscript "Gut microbiome patterns depending on children’s psychosocial stress: reports versus biomarkers". Gut microbiome sequencing was done in 93 Belgian children 8-16y old. Stress measures included negative events, negative emotions, emotional problems reported by parents, happiness, hair cortisol and heart rate variability (pnn50 parameter reflecting parasympathetic activity).

Raw data in excel file format, showing western blot, qPCR, and CPP