Metabolic Engineering
ISSN: 1096-7176
Datasets associated with articles published in Metabolic Engineering
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- What CHO is made of: Variations in the biomass composition of Chinese hamster ovary cell linesData from exponential phase of batch cultures of Chinese Hamster Ovary (CHO) cells (13 cell lines / conditions). It contains biomass composition data at one time point in mid-exponential phase (dry mass, protein, lipid, DNA, RNA and carbohydrate content, amino acid and lipid composition), which shows variability of the composition among different cell lines/conditions. Extracellular concentrations of metabolites (amino acids, glucose, lactate, ammonia) were measured throughout the exponential phase and the uptake/secretion rates were calculated. Flux balance analysis was performed to see the impact of cell line specific biomass composition and uptake/secretion rates (calculated with different sampling frequencies) on the predictions of growth rates. Data is structured according to figures in the associated manuscript. For more detailed descriptions of the files see "read_me.txt" files in each folder.
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- Data for: Connecting central carbon and aromatic amino acid metabolisms to improve de novo 2-phenylethanol production in Saccharomyces cerevisiae.Strains, primers, numerical data of data in graphs and statistical data
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- Data for: A concerted systems biology analysis of aromatic metabolism in Rhodococcus opacus PD630Flux Analysis Raw Data
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- Data for: Growth and selection of the cyanobacterium Synechococcus sp. PCC 7002 using alternative nitrogen and phosphorus sourcesReport from the NTU Phenomics Center on HPLC-MS/MS quantification of melamine, ammeline, ammelide and cyanuric acid in growth medium, at different time points.
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- Data for: Knockdown of carbonate anhydrase elevates Nannochloropsis productivity at high CO2 levelHere carbonic anhydrase (CA2) gene sequence was provided; DNA sequence for RNAi vector was provided; Transcriptomic and proteomic data from wildtype and CA2-knockdown line were provided.
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- Data for: Sugar phosphate analysis with baseline separation and soft ionization by gas chromatography-negative chemical ionization-mass spectrometry improves flux estimation of bidirectional reactions in cancer cellsThis document incudes mass spectra data of PFBO- and TMS-derivatized sugar phospahtes and details of 13C-metabolic flux analysis of MCF-7 human breast cancer cell line.
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- Data for: Application of a curated genome-scale metabolic model of CHO DG44 to an industrial fed-batch processTailored model of CHO DG44.
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- Data for: Dynamic modeling of subcellular phenylpropanoid metabolism in Arabidopsis lignifying cellsMatlab codes for the refined model of general phenylpropanoid pathway in Arabidopsis basal lignifying stems.
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- data for CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9This is the raw NGS sequencing data as python analysis for the paper thats currently titled: CasPER, a method for directed evolution in genomic contexts using mutagenesis and CRISPR/Cas9
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- Data for: Alterations in the transcription factors GntR1 and RamA enhance the growth and central metabolism of Corynebacterium glutamicumA summary of the genomic re-sequencing results can also be seen in Supplementary Data 1. The detailed information regarding the transcriptomics analysis is provided in Supplementary Data 2. The raw measured and fitted labelling data for metabolic flux analysis can be found in Supplementary data 3.
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