Forensic Science International: Genetics Supplement Series

The results of sequencing and additional STR test.

A table of three columns. Column one is a list of of fiftymers observed in two human samples and the other two giving their observed frequencies in the samples.

On degraded DNA samples a multiplex PCR mixture containing polynucleotide primers can provide superior genotyping of Short Tandem Repeats (STRs) to the current commercial kits based on standard oligonucleotides

Table a. STRs multiplex primers features

The allele frequency data for the 23 autosomal STR loci included in the VeriFiler Express PCR Amplification Kit obtained from 1,609 unrelated Caucasian individuals from different regions of the Russian Federation.

Data from the paper: Population genetic data of 38 insertion-deletion markers in South East Spanish population. M Saiz, MJ Alvarez-Cubero, LJ Martinez-Gonzalez, JC Alvarez, JA Lorente. Forensic Science International: Genetics (2014), 13; 236-8

The genotypes of 39 loci of alleged father, mother, and child from MicroreaderTM 23sp and PowerPlex® 21 STR kits.

This validation was aimed to demonstrate that there is no effect on the Applied Biosystems® 3500xL Genetic Analyzer (Thermo Fisher Scientific®, UK) after decommissioned and recommissioned at new premises. Same sample sets were tested on the ABI 3500xL Genetic Analyzer at both old and new premises using current accredited procedures. The results showed genotyping concordance which means using the ABI 3500xL Genetic Analyzer at the new premises provides no risk regarding the accuracy, precision and reproducibility of the results and also showed a very good performance on sensitivity, specificity, repeatability and good profile quality based on the assessment of locus peak balance and peak height.

The effect of decalcification on pulverised bone samples was studied to evaluate its value when processing reasonably good quality samples. The decalcified and non-decalcified bone samples were extracted using phenol-chloroform extraction method, quantitated with GoTaq® qPCR Master Mix (Promega®, UK) using Applied Biosystems® 7500 Real-Time PCR System (Thermo Fisher Scientific®, UK) and amplified using an in-house amplification multiplex. The results showed that the decalcification of bone samples is not a necessary when processing good quality samples and higher DNA yields were obtained without the decalcification process. The electropherograms produced from the extracted DNA samples without decalcification were good quality and displayed no PCR inhibition.

Inside the folder "Likelihood Ratios", multiple text files can be found which contain our obtained Likelihood Ratios for the 100,000 profiles of each kinship being studied, through the software "Familias". The Excel file compiles the tables which result from the comparison, through ratios, of the values obtained using each of the different models.