The data associated with the article "Extracting Phylogenetic Signal from Phylogenomic Data: Higher-Level Relationships of the Nightbirds (Strisores)" published in 2019 in Molecular Phylogenetics and Evolution by Noor D. White and Michael J. Braun includes the following files: Matrices_phylip.tar.gz: Concatenated nucleotide matrices used in this study, including character sets detailing individual UCE loci Tree files are those found in Figure 5 of the manuscript. They include: Trees_concatenated_ACGT.tar.gz: Results of the concatenated analysis of nucleotide matrices Trees_concatenated_RY.tar.gz: Results of the concatenated analysis of RY-coded matrices Trees_gene_tree_sum_ACGT.tar.gz: Results of the gene tree summarization of nucleotide matrices Trees_gene_tree_sum_RY.tar.gz: Results of the gene tree summarization of RY-coded matrices Trees_Low_GC_Variance.tar.gz: Results of the concatenated analysis of nucleotide matrices containing only UCE loci with low GC variance (10% highest GC variant loci removed) Trees_Part_by_Locus.tar.gz: Results of the concatenated analysis of nucleotide matrices partitioned by UCE locus