Topology and adenocarcinoma cell localization dataset on the labyrinthin biomarker

Published: 25 March 2022| Version 2 | DOI: 10.17632/pg7z5zkgc8.2
Contributors:
Michael Babich,
Ankit Sharma,
James Radosevich

Description

The data relate to analyses of labyrinthin, a pan-adenocarcinoma diagnostic and therapeutic neo-antigen. The data are included in the paper: Babich, M., Sharma, A., Li, T. and Radosevich, J.A. "Topology and adenocarcinoma cell localization dataset on the labyrinthin biomarker", bioRxiv, 2021 (and submitted to the journal Data in Brief). The data are supplemental to the paper: Babich, M., Sharma, A., Li, T. and Radosevich, J.A. "Labyrinthin: A distinct pan-adenocarcinoma diagnostic and immunotherapeutic tumor specific antigen. Heliyon, 2022. Specifically, the data are on the 1) topology, 2) sequence analyses, 3) and cell surface localization of labyrinthin and are presented as evidence in support of labyrinthin as a pan-adenocarcinoma marker. The files posted herein include raw information that led to information summarized as follows: Multiple bioinformatics tools were used to collectively predict calcium binding domain(s), N-myristoylation sites, kinase II phosphorylation sites, and transmembrane domain(s) with predicted N- and C-termini orientations. Amino acid (a.a.) sequence homology comparisons were found and alignments made for labyrinthin (255 a.a.) vs. the intracellular aspartyl/asparaginyl beta-hydroxylase (ASPH; 758 a.a.) and the ASPH-gene related protein junctate (299 a.a.), which are both type II proteins. The epitopes for commonly used MCA 44-3A6 anti-labyrinthin and FB-50 anti-ASPH/junctate antibodies were present in all three proteins. The selective expression of labyrinthin was investigated by third party fluorescent activated cell sorter (FACS) analyses of MCA 44-3A6 binding to non-permeabilized adenocarcinoma and normal cell line models. Labyrinthin was detected on non-permeabilized A549 human lung adenocarcinoma cells, but not on normal WI-38 human lung fibroblasts nor primary cultures of normal human glandular-related cells. Microscopic images of immunofluorescent labelled MCA 44-3A6 binding to A549 cells at random cell cycle stages complement the FACS results by further showing that labyrinthin persisted on the cell surfaces along with some cell internalization within 20 minutes.

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Categories

Biomarkers, Adenocarcinoma

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