Genome-wide screens implicate loss of cullin ring ligase 3 in persistent proliferation and genome instability in TP53 deficient cells

Published: 6 November 2020| Version 3 | DOI: 10.17632/pm5v6pwkkg.3
Contributor:
Alexandros Drainas

Description

TP53 deficiency is the most common alteration in cancer, however, alone is typically insufficient to drive tumorigenesis. To identify genes promoting tumorigenesis in combination with TP53 deficiency, we performed genome-wide CRISPR/Cas9 knockout screens coupled with proliferation and transformation assays in isogenic cell lines. Loss of several known tumor suppressor genes enhanced cellular proliferation and transformation. Surprisingly, loss neddylation pathway genes, promoted uncontrolled proliferation exclusively in TP53-deficient cells. CUL3 coupled with TP53 loss results in an oncogenic transcriptional program governed by the NFκB, AP-1 and TGF-β pathways. This program maintains persistent cellular proliferation, induces partial epithelial to mesenchymal transition, increases DNA damage, genomic instability, and the rate chromosomal rearrangements. Our findings reveal CUL3 loss as a key event stimulating persistent proliferation in TP53 deficient cells. These findings may be clinically relevant, since TP53-CUL3 deficient cells are highly sensitive to ATM inhibition, exposing a vulnerability that could be exploited for cancer treatment.

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Figure 2: R7,R8,R24 are 3 different sgRNAs for CUL3, SCR is scramble Figure 4: A1,A2,A3 are CUL3 knockout cell lines, A4,A5,A6 are wild type

Institutions

Stanford Medicine

Categories

Western Blot, Cellular Imaging

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