Data for: Microarray data on the comparison of transcript expression between normal and Pt-Delta RNAi embryos in the common house spider Parasteatoda tepidariorum

Published: 27 April 2019| Version 1 | DOI: 10.17632/r79vg2ctr2.1
Contributor:
Hiroki Oda

Description

We conducted a custom microarray experiment to detect the differences in the transcript expression levels between untreated (normal) and Pt-Delta-RNAi and embryos at late stage 6 in the common house spider Parasteatoda tepidariorum. The array probes were designed based on accumulated EST and cDNA sequences. The microarray dataset has been deposited in the Gene Expression Omnibus (GEO) Database at the National Center for Biotechnology Information (NCBI) under the accession GSE113064. EST clones that showed the ratio of [Pt-Delta RNAi] / [normal] of < 0.6 for at least one array probe were selected. The expression of the transcripts related to these EST clones was examined in normal embryos at stages 5−8 by whole-mount in situ hybridization. The .zip file contains raw images showing the stained embryos.

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Categories

Arthropoda, Gene Expression, Model Organism, Animal Embryo

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