The actin nucleator Cobl is critical for centriolar positioning, postnatal planar cell polarity refinement and function of the cochlea - Haag et al

Published: 30 August 2018| Version 1 | DOI: 10.17632/rypw3jv8mj.1
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Michael Kessels

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Original data sets of figures being part of the scientific study "The actin nucleator Cobl is critical for centriolar positioning, postnatal planar cell polarity refinement and function of the cochlea" (see https://doi.org/10.1016/j.celrep.2018.07.087) by Natja Haag1§, Susann Schüler1, Sandor Nietzsche2, Christian A. Hübner3, Nicola Strenzke4, Britta Qualmann1*, Michael M. Kessels1*# 1Institute of Biochemistry I, 2Electron Microscopy Center and 3Institute of Human Genetics, Jena University Hospital – Friedrich Schiller University Jena, Germany 4InnerEarLab, Department of Otolaryngology, University of Göttingen, Germany Author list Footnotes: #Lead Contact; §present address: Institute of Human Genetics, University Hospital RWTH Aachen, Germany *Correspondence: Britta.Qualmann@med.uni-jena.de & Michael.Kessels@med.uni-jena.de Summary Proper cochlear hair cell array development and sensory apparatus positioning are achieved by planar cell polarity signaling. Effectors executing proper tissue development and maturation programs are largely unknown. We show that the actin nucleator Cobl is an important effector in postnatal refinement and maintenance of planar cell polarity. During the critical time of hearing onset these polarity defects coincided with reduced F-actin beneath the sensory apparatus and with premature kinocilium retraction. The latter was preceded andThese defects were accompanied by organizational defects of the pericentriolar scaffold that coincided with basal body and centriolar mispositionings. in relation to the kinocilium and the stereocilia bundle. Also basal body/centriolar positioning was affected. Importantly, the pericentriolar defects observed in Cobl KO mice were were demonstrated to be actin polymerization-dependent and calcium/calmodulin signaling-dependent. As Because Cobl KO phenotypes manifested postnatally, planar cell polarity is not solely an important developmental process. The but needs active Cobl-dependent planar cell polarity maintenance and refinement processes we describe here postnatally. These cell biological processes seem critical for hearing. , as Cobl KO mice show deficient cochlear amplification.

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