Contributors: Lior Nissim, Ming-Ru Wu
... The data deposited here contains all the plasmid sequences, key raw data supporting this study, and information on key resources used in this study
Contributors: Peter St George-Hyslop
... Raw data for Figures 1-7
Supplementary datasets: Multiplexed proteome dynamics profiling reveals mechanisms controlling protein homeostasis, Savitski et al.
Contributors: Nico Zinn, Mikhail Savitski, Maria Faelth-Savitski, Marcus Bantscheff, Giovanna Bergamini
... Savitski et al Figure 2.zip Description: mPDP of THP1 cells treated with JQ1-VHL PROTAC Content: - Figure 2.pdf - Figure S2.pdf - Supplementary dataset 1_mPDP JQ1-VHL PROTAC.xlsx Savitski et al Figure 3.zip Description: 2-dimensional Thermal proteome profiling (2D-TPP) of THP1 cells treated with JQ1 Content: - Figure 3.pdf - Figure S3.pdf - Supplementary dataset 1_mPDP JQ1-VHL PROTAC.xlsx Savitski et al Figure 4.zip Description: mPDP of estrogen signaling and selective estrogen receptor modulators (SERMs) Content: - Figure 4.pdf - Figure S4.pdf - Supplementary Dataset 3_Estrogen.xlsx Savitski et al Figure 5.zip Description: mPDP of HSP inhibitor 17-AAG in Jurkat and MCF7 cells Content: - Figure 5.pdf - Figure S5.pdf - Supplementary Dataset 3_Estrogen.xlsx - Supplementary Dataset 4_17AAG.xlsx - Supplementary Dataset 5_HSP90 dep etc.xlsx - Supplementary Dataset 6_reference_melting curves.xlsx - Supplementary Dataset 7_kinobeads_activatedLysate.xlsx Savitski et al Figure 6-7.zip Description: mPDP of HSP inhibitor 17-AAG in primary T-cells Content: - Figure 6.pdf - Figure S6.pdf - Figure 7.pdf - Figure S7.pdf - Supplementary Dataset 5_HSP90 dep etc.xlsx - Supplementary Dataset 6_reference_melting curves.xlsx - Supplementary Dataset 8_Tcells.xlsx
Contributors: Manajit Hayer-Hartl, Xiao Yan, Qiaoyun Shi, Andreas Bracher, Goran Miličić, Amit Singh, F. Ulrich Hartl
... Raw data for figures published in Cell 172 (2018): Title: "GroEL Ring Separation and Exchange in the Chaperonin Reaction" Authors: Xiao Yan, Qiaoyun Shi, Andreas Bracher, Goran Miličić, Amit K. Singh, F. Ulrich Hartl and Manajit Hayer-Hartl. DOI: http://doi.org/10.1016/j.cell.2017.12.010
Contributors: Ilaria Piazza
... Supplementary analyzed data from Manuscript: TITLE: A map of protein-metabolite interactions reveals principles of chemical communication. JOURNAL: CELL. Article Type: Resource Article First Author: Ilaria Piazza Authors: Ilaria Piazza, Karl Kochanowski, Valentina Cappelletti, Tobias Fuhrer, Elad Noor, Uwe Sauer, Paola Picotti. Corresponding Author: Paola Picotti FILE: Piazza_SupplDataS8: Supplementary_DataAnalysis_S8. Related to Figure 6 and S6. Differential SEC-MS elution profiles upon ATP binding of ATP binding proteins undergoing large structural changes found by LiP-SMap. FILE: Piazza_SupplDataS9: Supplementary_DataAnalysis_S9. Related to Figure S6. Differential SEC-MS elution profiles upon ATP binding of proteins that do not bind ATP. FILE: Piazza_SupplDataS10: Supplementary_DataAnalysis_S10. Related to Figure 7. Concentration dependent local structural response curves relative to single conformotypic peptides.
Contributors: Raj Gupta
... RNA-seq in iPSC-derived ECs with editing at rs9349379.
Cytosolic protein Vms1 links ribosome quality control to mitochondrial and celluler homeostasis. Izawa and Park et al.
Contributors: Walter Neupert
... Images for immunoblots
Contributors: Scott Widenmaier
... Cholesterol is a critical nutrient requiring tight constraint in the endoplasmic reticulum (ER) due to its uniquely challenging properties. While the mechanisms by which ER defends against cholesterol insufficiency are well described, it remains unclear how ER senses and effectively defends against cholesterol excess. Here, we identify the ER-bound transcription factor nuclear factor erythroid 2 related factor-1, Nrf1/Nfe2L1, as a critical mediator of this process. We show Nrf1 directly binds to and specifically senses cholesterol in the ER through a defined domain, and that cholesterol regulates Nrf1 turnover, processing, localization, and activity. In Nrf1 deficiency, in vivo cholesterol-challenges induce massive hepatic cholesterol accumulation and damage, which is rescued by replacing Nrf1 exogenously. This Nrf1-mediated mechanism involves the suppression of CD36-driven inflammatory signaling and de-repression of liver X receptor activity. These findings reveal Nrf1 as a guardian of cholesterol homeostasis and a core component of adaptive responses to excess cellular cholesterol.
Contributors: L.J. Sparvero
... MALDI-MS imaging raw data for Supplemental Figures of Wenzel et al, "PEBP1 Wardens Ferroptosis by Enabling Lipoxygenase Generation of Lipid Death Signals." The data is readable with Bruker Daltonics FlexImaging software (www.bruker.com) or MSiReader sofware (www.msireader.com).
Contributors: Michael Muelleder, Markus Ralser
... This data-set represents a genome-scale metabolomic map about amino acid metabolism in gene deletion strains as determined in Saccharomyces cerevisiae.