Contributors: Koseki Kobayashi-Kirschvink, Hidenori Nakaoka, Arisa Oda, Ken-ichiro Kamei, Kazuki Nosho, Hiroko Fukushima, Yu Kanesaki, Shunsuke Yajima, Haruhiko Masaki, Kunihiro Ohta
... Raw data and analysis codes for paper entitled "Linear Regression Links Transcriptomic Data and Cellular Raman Spectra".
Contributors: Stephane Chevrier, Vito Zanotelli
... Raw mass cytometry and imaging mass cytometry data used to develop the compensation tool available within the R package CATALYST
Contributors: daniel schultz
... Data_S1 is a Matlab file containing the growth curves of six liquid-culture experiments used in Figures 1 and 2, obtained with an automated robotic system at 30ºC. The 'Experiment' field includes a reference to the panels in the main text where the data is used, and the 'Info' field specifies how tetracycline was added to the cultures. The 'Data' field contains optical density (OD) and fluorescence (when applicable) measurements for each strain used in the experiment, together with vectors specifying the gradients of IPTG and tetracycline used (when applicable), the time of the measurements, indication of each type of measurement performed and times of IPTG induction (in Fig. 1F). A detailed description of the methods and strains used is given in the STAR Methods section. Data_S2 contains the image files obtained with the microfluidic device, where cells carrying the native tet resistance mechanism were exposed to a step increase of 70μg/ml tetracycline at time zero. Images were taken every 10 minutes at 10 different positions in 3 different fluorescence channels. CFP was expressed constitutively and used for image segmentation. GFP was expressed from the same promoter as TetR and mCherry was expressed from the same promoter as TetA. The file name indicates the time, position and fluorescence channel. A detailed description of the experiment and the strain used is given in the STAR Methods section.
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Contributors: Lee Dunham
... Matlab scripts for the Refractory Model, Dunham et al.
Contributors: Patrick Stumpf
... Single-cell gene expression data generated using 96x96 fluidigm dynamic arrays. Experimental details including steps to reproduce data are included in the associated manuscript. Briefly, mouse embryonic stem cells kept in 2i+LIF conditions for four passages differentiated towards the neuronal lineage in N2B27 medium as described in the literature (Ying et al. 2003 - DOI: 10.1038/nbt780). At 0h, 24h, 48h, 72h, 96h, 120h and 168h individual cells were sorted by FACS based on light-scatter properties and deposited into 96-well plates containing lysis buffer and RT reagents before processing for qPCR using the Biomark HD.
Contributors: Olga Ponomarova
... Amino acids in CDM35 medium conditioned with five different S. cerevisiae strains. Only amino acids not present in CDM35 were quantified. Concentrations are given in nM, corresponding OD600 values are also provided.
Contributors: Christoph Rau
... Coexpression Networks generated via wMICA on the HMDP Heart Failure Study.
Contributors: Christoph Rau
... Phenotypic data from the HMDP Heart Failure Study
Contributors: Zachary Pincus
... Raw image file data for each specific measurement and time point is within individual .zip files. An overview file, PhysiologyGalleries.pdf, with additional galleries of C. elegans physiological measurements is included for ease of viewing. Compliance with Mendeley Data Policies: This is not a section of a previously published paper. It contains annotated and raw image data related to a paper in Cell Systems, but is distinct from and is not contained in that publication. The image data found here has not been previously published elsewhere.