Contributors: Mangal Singh, sandeep kaka, Dipti Sareen
... Lantibiotics are lanthionine ring containing natural products that belong to the class of ribosomally synthesized and post-translationally modified peptides (RiPPs). Recent expansion in the availability of microbial genome data and in silico analysis tools, have accelerated the discovery of these promising alternatives to antibiotics. Following the genome-mining approach, a biosynthetic gene cluster for a putative two-component lantibiotic, roseocin, was identified in the genome of an Actinomycete, Streptomyces roseosporus NRRL 11379. Post-translationally modified lanthipeptides of this cluster were obtained by heterologous expression of the genes in E. coli, and were in vitro reconstituted to their bioactive form by exploiting commercial proteases like endoproteinase GluC, and proteinase K. The two peptides displayed synergistic antimicrobial activity against Gram-positive bacteria including the WHO high priority pathogens, MRSA and VRE. Structural characterization confirmed the installation of four (methyl)lanthionine rings with an indispensable disulphide bond in the α-peptide, and six (methyl)lanthionine rings in the β-peptide, by a single promiscuous lanthionine synthetase, RosM. Roseocin is the first two-component lantibiotic from a non-firmicute, with extensive lanthionine bridging.
Contributors: Chang Yu Sung
... dataset for "A Two Year Survey for VLF Emission from Fireballs" raw data in this dataset was gathered by AWESOME system located in Elginfield Observatory located in southern Ontario, Canada. all event spectrogram folder contains the lightcurve/+-2s/+-5s/+-15s spectrogram for all 80 events for rough inspection. no phenomenologically different signals were found in these events non positively correlated events folder contains the 7 events where calculated signal direction matches recorded meteor, and correlated lightning was not recorded in the NLDN database. in this folder, the 2 .mat files are the raw signals for NS(A) and EW(B) channel of AWESOME antenna, filename denotes its time. the offset between AWESOME antenna and ASGARD-SOMN is roughly 1.23+-0.03s. phi.txt in each folder records (in order) the (1)beginning azimuth, (2)ending azimuth, (3)start of luminous flight compared to peak brightness, (4)end of luminous flight compared to peak brightness, and (5)exact time of peak brightness within the second(raw data is only trimmed to be centered around the start of the second of the peak brightness, not the exact time of the peak brightness). The subfolders are named as "sample number of the peak/binsize of spectrogram/time of peak relative to the time of peak brightness", and each subfolder contains +-2ms amplitude, +-10ms amplitude, direction fit, and location within luminous flight of the particular fit.
Reshaping commensal gut microbiota in early life with amoxicillin presents with lower blood pressure
Contributors: Saroj Chakraborty
... Pediatric hypertension is recognized as an emerging global health concern. While new guidelines are developed for facilitating clinical management, the reasons for the prevalence of hypertension in children remain unknown. Genetics and environmental factors do not fully account for the growing incidence of pediatric hypertension. Because stable bacterial flora in early life are linked with health outcomes later in life, we hypothesized that reshaping of gut microbiota in early developmental stages of life affects blood pressure (BP) of pediatric subjects. To test this hypothesis, we administered amoxicillin, the most commonly prescribed pediatric antibiotic, to alter gut microbiota of young, genetically hypertensive rats (study 1) and dams during gestation and lactation to reshape microbiota of offspring (study 2). Reshaping of microbiota, with reductions in Firmicutes/Bacteriodetes ratio observed in Amoxicillin treated young rats and in dams. Amoxicillin treated rats also had lower blood pressure compared to the untreated rats. In the young rats treated with amoxicillin, the lowering effect on blood pressure persisted even after the antibiotics were discontinued. Similarly, the offspring from the dams treated with amoxicillin also showed lower systolic blood pressure compared to the control rats. Remarkably, in all cases, a decrease in BP was associated with lowering of Veillonellaceae, which are succinate-producing bacteria. Elevated plasma succinate is reported in hypertension. Accordingly, serum succinate was measured and found lower in animals treated with amoxicillin. Our results demonstrate a direct correlation between succinate-producing gut microbiota and early development of hypertension, and indicate that reshaping gut microbiota, especially by depleting succinate-producing microbiota early in life may have long-term benefits for hypertension-prone individuals.
Contributors: Matthias Sinnesael, Sietske J. Batenburg, Christian Zeeden, Jiri Laurin, Damien Pas, Linda Hinnov, Johannes Monkenbusch, Maximilian Vahlenkamp, Mingsong Li, Sébastien Wouters
... Supplementary materials for Submission: Sinnesael et al., Earth-Science Reviews "The Cyclostratigraphy Intercomparison Project (CIP): consistency, merits and pitfalls ".
Contributors: Zhanni Luo
... This is an eye-tracking experiment to investigate whether visualizers and verbalizers present different eye-movement patterns in viewing documents with different arrangements of images and text. Combined with the questionnaire and interview approach, the current study explored the accuracy of using eye-tracking technology to identify visualizers and verbalizers, as well as possible factors contributing to different levels of accuracy.
Data on vibrational spectra of the langasites Ln3CrGe3Be2O14 (Ln = La, Pr, Nd) and ab initio calculations
Contributors: Nikolai Kuzmin, Sergey Klimin, Boris Mavrin, Kirill Boldyrev, Vladimir Chernyshev, Boris Mill, Marina Popova
... The data presented in this article includes IR diffuse transmission spectra of powder samples of Ln3CrGe3Be2O14 (Ln = La, Pr, Nd) registered at room temperature with a Bruker 125HR Fourier spectrometer, Raman spectra taken in the backscattering geometry (also at room temperature) with a triple monochromator using the line 514,5 nm of an argon laser as an excitation, results of the DFT calculations with the B3LYP and PBE0 hybrid functionals on the optimized crystal structures, eigenfrequencies and eigenvectors of the normal vibrational modes. These data can be used to analyse electron-phonon interaction and multiferroic properties of the new langasites and to compare the lattice dynamics of different langasites.
Data for: Design of a red-emitter hybrid material for bioimaging: europium complexes grafted on silica particles.
Contributors: Alessandra Mara Mutti
... Treated and untreated data from a paper called "Design of a red-emitter hybrid material for bioimaging: europium complexes grafted on silica particles" - Materials Today Chemistry
Dataset on tip vortex formation noise produced by wall-mounted finite airfoils with flat and rounded tip geometries
Contributors: Tingyi Zhang, Danielle Moreau, Thomas Geyer, Con Doolan
... This dataset consists of measurements of tip vortex formation noise produced by finite-length airfoils with flat and rounded tips. These data were obtained using the specialist aeroacoustic test facilities at the Brandenburg University of Technology (BTU) in Cottbus, Germany and a 47-channel planar microphone array. Over 1200 unique test cases with variations in airfoil profile shape, tip geometry, angle of attack and Reynolds number were measured during the experimental campaign. The dataset contains one-third-octave band tip noise spectra that have been processed using Acoular, a Python module for acoustic beamforming.
Contributors: albert nguyen van nhien
... we present the extraction and chemical composition of chitins of different purity grade from an insect source so called Bombyx eri larva
Data/Software for "Presynaptic Mitochondria Volume and Abundance Increase During Development of a High-Fidelity Synapse"
Contributors: Connon I. Thomas, Christian Keine, Satoko Okayama, Rachel Satterfield, Morgan Musgrove, Debbie Guerrero-Given, Naomi Kamasawa, Samuel M. Young, Jr.
... Contains data and software from the publication: "Presynaptic Mitochondria Volume and Abundance Increase During Development of a High-Fidelity Synapse" published in the The Journal of Neuroscience (https://doi.org/10.1523/JNEUROSCI.0363-19.2019). The preprint to this data set has been published on bioRxiv (https://doi.org/10.1101/689653). In this study, we created a helper-dependent adenoviral vector (HdAd) to co-express cytoplasmic EGFP and a genetically encoded peroxidase marker (mito-APEX2) at the calyx of Held, an excellent model for deciphering regulatory mechanisms of presynaptic function. ABSTRACT: The calyx of Held, a large glutamatergic presynaptic terminal in the auditory brainstem undergoes developmental changes to support the high action-potential firing rates required for auditory information encoding. In addition, calyx terminals are morphologically diverse which impacts vesicle release properties and synaptic plasticity. Mitochondria influence synaptic plasticity through calcium buffering and are crucial for providing the energy required for synaptic transmission. Therefore, it has been postulated that mitochondrial levels increase during development and contribute to the morphological-functional diversity in the mature calyx. However, the developmental profile of mitochondrial volumes and subsynaptic distribution at the calyx of Held remains unclear. To provide insight on this, we developed a helper-dependent adenoviral vector (HdAd) that expresses the genetically encoded peroxidase marker for mitochondria, mito-APEX2, at the mouse calyx of Held. We developed protocols to detect labeled mitochondria for use with serial block face scanning electron microscopy to carry out semi-automated segmentation of mitochondria, high-throughput whole terminal reconstruction and presynaptic ultrastructure in mice of either sex. Subsequently, we measured mitochondrial volumes and subsynaptic distributions at the immature postnatal day 7 (P7) and the mature (P21) calyx. We found an increase of mitochondria volumes in terminals and axons from P7 to P21 but did not observe differences between stalk and swelling subcompartments in the mature calyx. Based on these findings, we propose that mitochondrial volumes and synaptic localization developmentally increase to support high firing rates required in the initial stages of auditory information processing. Data are sorted by the figures they appear in. Media (movies and 3D models) and custom-written software are located in separate folders.