Contributors: Wenchu Li, Baoli Qiu, Yangjin Zhong
... Hypothesis: Embed teaching pedagogies is better than flipped classroom alone. The single factors of revised Bloom's digital knowledge dimensions might exist satatistical relationship. Supposing one color of hats principally affect the thinking results of “Six Thinking Hats”, it is interesting to confirm. Students’ satisfaction is principally affected by innovative thinking. Abstract: To explore a bilingual course of Insect Physiology and Biochemistry, we have, in recent years, tried to embed teaching pedagogies, such as a flipped classroom that combines brainstorming and “Six Thinking Hats”, in undergraduate education. The Statistical Product and Service Solutions (SPSS) analysis revealed that there are no significant differences between the final test scores of the classes. Interestingly, a revised analysis of Bloom's digital knowledge dimensions showed that the knowledge dimension exhibited procedure collinearity, and Factual negatively affected Metacognitive in both the Toulmin’s practical argument and control groups. The addition of “Six Thinking Hats” generated higher quality and more creative ideas than a flipped classroom alone, and it promoted the students to consider the issues more quickly. We confirmed that the participants under the yellow and green hats generated higher quality ideas than the participants under the red hats in the creative and innovative thinking model. Partial least squares structural equation modeling analysis of the effect of teaching confirmed the reflective model and data at satisfactory validity and suggested that essential teaching procedures have a nearly equal influence on the higher-order construction of brainstorming and innovative thinking. Skill has a strong effect on brainstorming. Similarly, we also confirmed that brainstorming has a strong effect on innovative thinking. However, students’ satisfaction is principally affected by innovative thinking but not by brainstorming.
Contributors: Ali Nili, Hamidreza mahmoudi
... Comparing the efficacy and the safety of KOH 5% solution with 5-Fluorouracil cream on patients suffering Actinic Keratosis: A Randomized controlled trial.
Contributors: Mangal Singh, sandeep kaka, Dipti Sareen
... Lantibiotics are lanthionine ring containing natural products that belong to the class of ribosomally synthesized and post-translationally modified peptides (RiPPs). Recent expansion in the availability of microbial genome data and in silico analysis tools, have accelerated the discovery of these promising alternatives to antibiotics. Following the genome-mining approach, a biosynthetic gene cluster for a putative two-component lantibiotic, roseocin, was identified in the genome of an Actinomycete, Streptomyces roseosporus NRRL 11379. Post-translationally modified lanthipeptides of this cluster were obtained by heterologous expression of the genes in E. coli, and were in vitro reconstituted to their bioactive form by exploiting commercial proteases like endoproteinase GluC, and proteinase K. The two peptides displayed synergistic antimicrobial activity against Gram-positive bacteria including the WHO high priority pathogens, MRSA and VRE. Structural characterization confirmed the installation of four (methyl)lanthionine rings with an indispensable disulphide bond in the α-peptide, and six (methyl)lanthionine rings in the β-peptide, by a single promiscuous lanthionine synthetase, RosM. Roseocin is the first two-component lantibiotic from a non-firmicute, with extensive lanthionine bridging.
Data for: Expression of the autophagy-related protein LC3B in granulosa cells, cumulus cells, and oocyte during atresia of pig antral follicles
Contributors: Luisa Gioia, Alessia Gloria, Luca Valbonetti, Alberto Contri, Alessandro Colapietro, Claudio Festuccia
... For a long time, in mammals apoptosis was accepted as the type of programmed cell death (PCD) occurring in ovarian follicles undergoing atresia. However, recent studies have suggested that autophagy may be an alternative mechanism involved in follicle depletion, working independently or in tandem with apoptosis. This research investigated by western blot and immunofluorescence the expression of LC3B protein in freshly collected granulosa cells (GC), cumulus cells (CC), and oocytes to evaluate the involvement of autophagy in the process of antral follicle atresia in sexually mature sows. Moreover, apoptosis was analysed by Annexin V and TUNEL assays in the same cellular cohorts to evaluate the correlation between the two processes. Our immunostaining results show that the autophagy is induced in the majority of GC, CC, and oocytes collected from early and advanced atretic follicles. The quantitative results of western blot analysis demonstrate that a significant (P < 0.05) and progressive increase of expression of the autophagy-related form of the protein (LC3B-II) takes places in these cells compared to healthy ones. Moreover, our data confirm that apoptosis occurs in GC of atretic follicles, thus showing that in pigs, like in rats and humans, apoptosis and autophagy work in GC as combined processes of PCD for antral follicle depletion. Finally, we did not detect apoptosis whereas we detected an overexpression of LC3B-II in CC and oocytes of atretic follicles, thus we suggest that in these cells the two processes work independently, with autophagy acting like a cytoprotective rather than a PCD mechanism.
Reshaping commensal gut microbiota in early life with amoxicillin presents with lower blood pressure
Contributors: Saroj Chakraborty
... Pediatric hypertension is recognized as an emerging global health concern. While new guidelines are developed for facilitating clinical management, the reasons for the prevalence of hypertension in children remain unknown. Genetics and environmental factors do not fully account for the growing incidence of pediatric hypertension. Because stable bacterial flora in early life are linked with health outcomes later in life, we hypothesized that reshaping of gut microbiota in early developmental stages of life affects blood pressure (BP) of pediatric subjects. To test this hypothesis, we administered amoxicillin, the most commonly prescribed pediatric antibiotic, to alter gut microbiota of young, genetically hypertensive rats (study 1) and dams during gestation and lactation to reshape microbiota of offspring (study 2). Reshaping of microbiota, with reductions in Firmicutes/Bacteriodetes ratio observed in Amoxicillin treated young rats and in dams. Amoxicillin treated rats also had lower blood pressure compared to the untreated rats. In the young rats treated with amoxicillin, the lowering effect on blood pressure persisted even after the antibiotics were discontinued. Similarly, the offspring from the dams treated with amoxicillin also showed lower systolic blood pressure compared to the control rats. Remarkably, in all cases, a decrease in BP was associated with lowering of Veillonellaceae, which are succinate-producing bacteria. Elevated plasma succinate is reported in hypertension. Accordingly, serum succinate was measured and found lower in animals treated with amoxicillin. Our results demonstrate a direct correlation between succinate-producing gut microbiota and early development of hypertension, and indicate that reshaping gut microbiota, especially by depleting succinate-producing microbiota early in life may have long-term benefits for hypertension-prone individuals.
Contributors: Sangarani Sharungbam, Pranav Pandya
... Manipur, a land of the rising sun, is endowed with rich biodiversity and abundant natural resources. Despite inaccessibility, marginality and heterogeneity, the state has made good progress in agriculture and allied sectors. The presence of Mountains causes a barrier effect over the entire region which leads to a protective effect on the flora and fauna. This protective effect has been studied in this research.
Contributors: Matthias Sinnesael, Sietske J. Batenburg, Christian Zeeden, Jiri Laurin, Damien Pas, Linda Hinnov, Johannes Monkenbusch, Maximilian Vahlenkamp, Mingsong Li, Sébastien Wouters
... Supplementary materials for Submission: Sinnesael et al., Earth-Science Reviews "The Cyclostratigraphy Intercomparison Project (CIP): consistency, merits and pitfalls ".
Molecular Identification and Isolation of Antimicrobial, Phytochemical, Antioxidant Properties of Different Solvent Extracts from Wild Mushrooms
Contributors: MANJUSHA PGS
... The main objective of the present research work was to identify the unknown wild mushrooms based on morphological characteristics and DNA based sequencing by using universal primers ITS 1 and ITS 4 which were collected from Andhra Pradesh and Telangana. To test the potential of various extracts of mushrooms by using three different solvents (Methanol, Hot Water and Hexane). To elucidate antimicrobial properties, qualitative and quantitative estimation of phytochemical properties and antioxidant activity was determined by DPPH free radical scavenging activity. Then, IC50 values were determined. The four isolates were identified on the basis of morphological and molecular characterization was identified as Trametes versicolor, Daedaleopsis confragosa, Schizophyllum communae and Tyromyces chioneus. Three different solvents had been shown good antimicrobial activity against all pathogenic microorganisms. The hot water extract of T. versicolor exhibited more amount of phenol content (11.13±0.081) and ascorbic acid content (9.62±0.086). Methanol extract of S. communae showed high amount of flavonoids content (12.58±0.138). Methanol extract of T. chioneus exhibited more amount of β-carotene (3.54±0.010) and hot water extract of D. confragosa exhibited more amount of lycopene content (2.29±0.012). The DPPH free radical scavenging activity and IC50 values was much more exhibited by methanol extracts then followed by hot water extracts and hexane extracts of wild mushrooms they all have exhibited stronger antioxidant activity between (40-50 µg/ml). This comprehensive analysis demonstrates that these mushrooms having a viable source of natural antioxidants that can utilized for functional foods, nutraceutical’s applications with beneficial biological activities and implementation of new drug discovery.
Photodynamic therapy for cutaneous squamous cell carcinoma in situ: impact of anatomic location, tumor diameter and incubation time on effectiveness
Contributors: Sean Christensen, Nour Kibbi, David Leffell, Yuemei Zhang
... Background Photodynamic therapy (PDT) has been reported as a treatment for cutaneous squamous cell carcinoma in situ (SCCis) but there is limited data on the effectiveness of PDT with aminolevulinic acid (ALA-PDT). Objective To review the outcomes of SCCis treated with ALA-PDT and examine factors associated with response. Methods Retrospective review identified 58 patients with 68 primary SCCis lesions treated with ALA-PDT and blue light illumination. Patient demographics, lesion features, treatment details, clinical response, and subsequent recurrence were extracted from medical record review. Results Upon completion of PDT, the initial complete response rate was 77.9% and was not associated with the number of PDT treatments. On multivariate analysis, the factors associated with response were location on the face, tumor diameter <2 centimeters, and longer ALA incubation time. Lesions treated with a maximum incubation time of <3 hours had a 53.3% response compared with 84.9% for longer incubation. Subsequent recurrence of SCCis was noted in 7 of 53 cases (13.2%) at a median time of 11.7 months. Limitations This was a retrospective study performed at a single institution without systematic follow-up. Conclusions ALA-PDT may be an effective treatment for selected cases of SCCis. Effectiveness is impacted by anatomic location, tumor diameter, and ALA incubation time. This Dataset is Supplementary Data for the manuscript, "Photodynamic therapy for cutaneous squamous cell carcinoma in situ: impact of anatomic location, tumor diameter and incubation time on effectiveness." Authors: Nour Kibbi, MD, Yuemei Zhang, MD, MBA, David J. Leffell, MD, and Sean R. Christensen, MD, PhD Accepted for publication by Journal of the American Academy of Dermatology 2019 (JAAD-D-19-01312R1). Supplementary Figure 1. Response of SCCis according to number of PDT treatments. A, Initial complete response for all lesions and according to number of PDT treatments. B, Durable complete response at final follow-up for all lesions and according to number of PDT treatments. Supplementary Figure 2. Occlusion during PDT incubation period was significantly associated with incubation time. P value was calculated using the Spearman correlation. Supplementary Table. Factors associated with recurrence of SCCis after initial complete response.
Bridging sensory evaluation and consumer research for strategic leafy Brassica (Brassica oleracea) improvement
Contributors: Hannah Swegarden, Phillip Griffiths, Alina Stelick, Robin Dando
... Research data uploaded here provided the foundation for analysis of Qualitative Multivariate Analysis (QMA_FocusGroupObservations), descriptive analysis (QDA_Sessions123 and ConsumerClusterDemo_Kale2019), and consumer testing (ConsumerKale_FinalData). These data have been cleaned and formatted from raw datasets; they are ready for downstream analysis. Participant journal entries and raw data are available upon request.