Reshaping commensal gut microbiota in early life with amoxicillin presents with lower blood pressure
Contributors: Saroj Chakraborty
... Pediatric hypertension is recognized as an emerging global health concern. While new guidelines are developed for facilitating clinical management, the reasons for the prevalence of hypertension in children remain unknown. Genetics and environmental factors do not fully account for the growing incidence of pediatric hypertension. Because stable bacterial flora in early life are linked with health outcomes later in life, we hypothesized that reshaping of gut microbiota in early developmental stages of life affects blood pressure (BP) of pediatric subjects. To test this hypothesis, we administered amoxicillin, the most commonly prescribed pediatric antibiotic, to alter gut microbiota of young, genetically hypertensive rats (study 1) and dams during gestation and lactation to reshape microbiota of offspring (study 2). Reshaping of microbiota, with reductions in Firmicutes/Bacteriodetes ratio observed in Amoxicillin treated young rats and in dams. Amoxicillin treated rats also had lower blood pressure compared to the untreated rats. In the young rats treated with amoxicillin, the lowering effect on blood pressure persisted even after the antibiotics were discontinued. Similarly, the offspring from the dams treated with amoxicillin also showed lower systolic blood pressure compared to the control rats. Remarkably, in all cases, a decrease in BP was associated with lowering of Veillonellaceae, which are succinate-producing bacteria. Elevated plasma succinate is reported in hypertension. Accordingly, serum succinate was measured and found lower in animals treated with amoxicillin. Our results demonstrate a direct correlation between succinate-producing gut microbiota and early development of hypertension, and indicate that reshaping gut microbiota, especially by depleting succinate-producing microbiota early in life may have long-term benefits for hypertension-prone individuals.
Contributors: Zhen-zhen Zhang
... Primary giant cell tumor originating in the rib is rare, resembling giant cell tumor in the epiphyseal region of long tubular bones morphologically and immunologically. However, this lesion poses a great challenge for diagnosis due to uncommon clinical and radiological presentation and histological heterogeneity. Here we report two cases of huge giant cell tumor of ribs presented as soft tissue mass. The first patient presented as a large expanding soft tissue mass in the right thoracic cavity attached to the 4th and 5th ribs with bone destruction. The second patient showed an ill-defined soft tissue mass in the left retroperitoneum. The maximum diameter of the lesions are greater than 15cm. Apart from the typical lesion with giant cell tumor evenly distributed among the mononuclear cells, there are secondary change including multiple hemorrhage cyst formation, irregular ossification and spindle cell proliferation, which mimics malignancies. H3.3 p.G34W immunohistochemistry and H3F3A mutation detected by Sanger sequencing supported the diagnosis of giant cell tumor. Our studies emphasized radiological and histological polymorphism and the diagnostic value H3F3A mutation, with a review of other published reports to assess radiological and pathological diagnosis and outcomes in case of misdiagnosis and overtreatment.
Contributors: Young-Min Soh, Iain Finley Davidson, Stefano Zamuner, Jerome Basquin, Michael Taschner, Florian Patrick Bock, Jan-Willem Veening, Paolo De Los Rios, Jan-Michael Peters, Stephan Gruber
... Dracala spotting assay, ITC measurements, Malachite Green Assay, in vitro and in vivo crosslinking , DNA loading assay, Sequence alignment used for DCA, Single Molecule Imaging
Contributors: Jeny Bastida, Alejandro Crampet, Melitta Meneghel, Victor Morais
... This dataset correspond to the proteomic analysis of the article entitle “Preliminary Biochemical and Venomic Characterization of the Venom of Phalotris lemniscatus (Serpentes, Colubridae)” sending to Current topics in medicinal chemistry. The data set include the raw files of each band of mass spectroscopy and the database used to identify the proteins. To observe the SDS page, please refer to the article. “Protein bands were excised and sent to the Spectroscopy and Biophysics Core, University of Nebraska, Lincoln (via Science Exchange) to in-gel trypsin digestion and peptide fragmentation by LC-MS/MS. The instrument was an LTQ Velos Pro (Thermo Scientific) equipped with dual pressure ion-trap. Raw data obtained by proteomic analysis was analyzed using MaxQuant software [30–32] with the default parameters (false discovery rate 0,01). As a sequence database to match for protein identification, a fasta file download from Uniprot was used. Database was constituted by all the reviewed sequenced proteins of snakes, around 2500 proteins from Swiss-Prot database (taxonomy:"Serpentes (snakes) " AND reviewed:yes)”.
Contributors: Esteban Finol Berrueta
... raw DENV genomic data for Finol E. & Ooi EE. Iscience submision
Contributors: UnaElsLive Natra, mdgmatest5 live
... RDM - File Type Support 21May2019 ElsCustomer Apart from .u3d all files preview [ .obj / .ply / .vtk / .stl / .ent / .brk / .pdb / .pse / .mol / .mol2 / .cif / .u3d / .dcm / .nii] - .pse is not supported
Data for: Dissection of protonation sites for antibacterial recognition and transport in QacA, a multi-drug efflux transporter
Contributors: Aravind Penmatsa, ashutosh gulati, Nazia Hussain, Puja Majumder, Arunabh Athreya, Shashank Khare
... The data comprises the raw data files of the manuscript titled "Dissection of protonations sites for antibacterial recognition and transport in QacA, a multi-drug efflux transporter", authored by Majumder et al.
Database related to the seasonal variability and trend analysis of the solar energy resource in Northeastern Brazilian region.
Contributors: Frnacisco Lima, Fernando Ramos Martins, Andre Rodrigues Gonçalves, Rodrigo Santos Costa, Enio Bueno Pereira
... Database generated produced during the evaluation study on the spatial patterns, seasonal variability and trend analysis of the surface solar irradiation in the Northeastern Brazilian region by using meteorological data acquires in automated weather stations operated by the Brazilian Institute of Meteorology from 2008 till 2015.
Data for: SUBDUCTION CONTROL ON THE CURIE ISOTHERM AROUND THE PACIFIC-NORTH AMERICA PLATE BOUNDARY IN NORTHWESTERN MEXICO (GULF OF CALIFORNIA). PRELIMINARY RESULTS.
Contributors: Oscar Campos Enriquez, erdinc oksum, Juan-Manuel Espinosa-Cardeña
... This repository contains the raw data necesay to reproduce our results. In particular, data necesary to reproduce the results shown in Figures 5a,5b, %fc, 6a, 6b, 6c, and 7a, and 7b are included, as well as instructions how to reproduce our results.
Supplementary Data S1: Next generation sequencing from Hepatozoon canis (Apicomplexa: Coccidia: Adeleorina): Complete apicoplast genome and multiple mitochondrion-associated sequences
Contributors: Alexandre Léveillé, John Barta
... These files comprise all of the NGS sequence assemblies referred to in the article: "Next generation sequencing from Hepatozoon canis (Apicomplexa: Coccidia: Adeleorina): Complete apicoplast genome and multiple mitochondrion-associated sequences." All assemblies were generated from Illumina HiSeq 2500 sequencing data (126 bp paired-end reads, insert length ~500 bp). In the case of mitochondrion-associated sequences 1, 2, 3 and 4: PCR and Sanger sequencing data were utilized to provide additional assembly coverage of CDS regions. Files included are: BAM assembly files: .bam, .bai and .fasta (these files are needed together to generate a BAM assembly flat file - supported by many software platforms). Geneious assembly files: Complete annotated assemblies (with NGS read pairings) can be viewed with Geneious software (versions 6.1 or newer). These files will provide the greatest details of the assembly data. Jpeg images of Geneious assemblies: These files were provided for ease of viewing and rapid analysis. Note: images were not generated for the complete ribosomal DNA unit and 18S rDNA variant assemblies as these assemblies were too large to viewed as images.