The human settlement of Central Iberia during MIS 2: New technological, chronological and environmental data from the Solutrean workshop of Las Delicias (Manzanares River valley, Spain)
Contributors: Manuel Alcaraz-Castaño, Mario López-Recio, Fernando Tapias, Felipe Cuartero, Javier Baena, Blanca Ruiz-Zapata, Jorge Morín, Alfredo Pérez-González, Manuel Santonja
... The recent excavations (2008–2009) conducted at the open-air site of Las Delicias, located in the Manzanares River valley (Madrid), have revealed new important data for the understanding of the human settlement of Central Iberia during Solutrean times. In this paper, we present a geomorphological and taphonomic study of the Pleistocene deposits of Las Delicias, a technological analysis focused on the bifacial lithic reduction processes documented at the site, new Optically Stimulated Luminescence dates, and new palynological data. Together with the existence of numerous Solutrean lithic assemblages from the early 20th century excavations of the Manzanares terraces, these new data highlight the importance of the Manzanares valley as a focus of Solutrean settlement, not only related to flint procurement but also to foraging activities. Moreover, they require reconsideration of Central Iberia as a virtually unpopulated region during the Late Pleniglacial (MIS 2), and of the associated idea of its cultural dependence on the coastal areas of the Iberian Peninsula. We propose new avenues of research aimed at approaching the central region of Iberia in its own cultural and ecological terms.
Archival Report - Neurochemical Mediation of Affiliation and Aggression Associated With Pair-Bonding
Contributors: Kyle L. Gobrogge, Xixi Jia, Yan Liu, Zuoxin Wang
... The neuropeptides vasopressin and corticotropin-releasing hormone facilitate, while serotonin inhibits, aggression. How the brain is wired to coordinate interactions between these functionally opposed neurotransmitters to control behavioral states is poorly understood.
Contributors: Qingdi Zhu, Dieter Trau
... Surface-tension-driven capillary systems (CSs) enable self-powered delivery of samples and reagents for bioassays and thus are especially suitable for point-of-care applications. Current silicon and polymer based CSs require extensive work in professional cleanroom for the fabrication of either the silicon device itself or the micromold for polymer processing. In this work, we fabricated a PEG-based CS in a one-step photopolymerization process without the requirement of any cleanroom work. Water, buffer and serum can flow autonomously in this CS and the liquid flow rate can be tuned by modification with surfactant solution of different concentrations. We further integrated an antibody-coated microbead array into this CS for the autonomous immunoassay of two tumor markers, prostate specific antigen (PSA) and human chorionic gonadotropin (hCG), in serum samples with a total assay time of less than 10min. The detection limits for the two tumor markers were at sub-nanogram per milliliter range which is lower than their clinical threshold concentrations for cancer diagnosis. Moreover, simultaneous and multiplex detection of the two tumor markers was also achieved by using spatially encoded microbeads. This low cost and easy-fabricated CS enables fast, multiplex and autonomous immunoassay for protein markers and has the potential to be applied for point-of-care diagnostics on real clinical samples.
Contributors: Meiyu Gai, Johannes Frueh, Tieyan Si, Narsiu Hu, Gleb B. Sukhorukov, Qiang He
... The collision phenomena of self-propelled micro-plate motors are significantly different from classical life-less particles. The Janus micro-plate motors used in this study are 5 and 10μm in diameter and driven by catalytic decomposition of hydrogen peroxide. Periodically oscillating oxygen bubbles drive the motor in rugged straight or spiral motion. Since the inertia effect of mass is no longer important for slow motion in low Reynold number liquid, the momentum law of collision shows some new characteristics due to the constant self-propelling force. We found a bounce back collision and a linear collision between the bubbles of one motor and the micromotor plates as well as between 2 microplates and present a basic theoretical concept. In addition we show experimentally that these 2D particles are able to destroy emerging gas bubbles. More complex collision dynamics may exist for a higher concentration of self-propelled motors.
Contributors: Anton Tomšič, Meindert Palmen, Michel I.M. Versteegh, Frank van der Kley, Hans-Marc J. Siebelink, Remco R. Berendsen, Martin J. Schalij
... Transapical valve-in-valve mitral valve implantation has been described as an alternative treatment option in patients with degenerated bioprosthesis in the mitral position. We report the first case of prosthesis dislocation and migration into the left atrium following transapical valve-in-valve implantation. A new prosthesis was implanted using the same approach. The dislocated prosthesis was successfully removed through the left atrial appendage through an extended anterolateral thoracotomy without the use of cardiopulmonary bypass.
Case Report - Direct His-Bundle Pacing Improved Left Ventricular Function and Remodelling in a Biventricular Pacing Nonresponder
Contributors: Peiren Shan, Lan Su, Xiao Chen, Lei Xu, Xianda Ni, Weijian Huang
... The optimal pacing modality after atrioventricular junction (AVJ) ablation remains unclear. Herein, we describe the case of a heart failure patient who had AVJ ablation for chronic atrial fibrillation and received a cardiac resynchronization therapy defibrillator device. Because of the lack of clinical response to biventricular pacing, the device was revised with the addition of direct His bundle pacing, which resulted in significant improvement in functional status and left ventricular indices. This case illustrated direct His bundle pacing as an alternative for conventional biventricular pacing in some cardiac resynchronization therapy nonresponders who undergo AVJ ablation for atrial fibrillation and have an intact distal conduction system.
Electrospun soluble conductive polypyrrole nanoparticles for fabrication of highly selective n-butylamine gas sensor
Contributors: Alireza Akbarinejad, Arash Ghoorchian, Mahdie Kamalabadi, Naader Alizadeh
... A three-dimensional and highly porous polypyrrole (PPy) film was successfully coated onto a copper interdigital electrode (Cu-IDE) surface by electrospinning of soluble PPy nanoparticles. The chemical composition of PPy nanoparticles was analyzed using X-ray photoelectron spectroscopy (XPS) and fourier transform infrared spectroscopy (FT-IR). The Brunauer–Emmett–Teller (BET) analysis confirmed the porous nature of PPy nanoparticles. The field emission scanning electron microscopy (FE-SEM) images of polymer coated Cu-IDE revealed that PPy nanoparticles were assembled by electrical forces to form an outstanding honeycomb-like architecture. As a proof-of-concept demonstration of the functional properties of the electrospun PPy (Es-PPy) film, the polymer coated Cu-IDE was investigated as a sensing device for gas sensor. The as-prepared Es-PPy film proved to be a viable aliphatic amines sensing material with large response, low detection limit, fast response and good repeatability at a low operating temperature of 150°C. Moreover, the sensor demonstrated an extremely high sensitivity and selectivity to n-butylamine. The calibration sensitivity to n-butylamine is up to three orders of magnitude higher than that of other common aliphatic amines. The detection limit and linear range for determination of n-butylamine were 0.42ppm and 10.54-21.08ppm, respectively. Es-PPy gas sensor exhibited good repeatability with RSD≤8% at temperature ranges 90–200°C. The response of the Es-PPy sensor to n-butylamine was compared with electrochemically and drop coated sensors and found that it has an extremely higher response. Finally, the Es-PPy gas sensor was successfully applied to real well water sample analysis.
Prefrontal cortex corticotropin-releasing hormone receptor 1 conveys acute stress-induced executive dysfunction
Contributors: Andrés Uribe-Mariño, Nils C. Gassen, Maximilian F. Wiesbeck, Georgia Balsevich, Sara Santarelli, Beate Solfrank, Carine Dournes, Gabriel R. Fries, Merce Masana, Christiana Labermeier
... The medial prefrontal cortex (mPFC) subserves complex cognition and is impaired by stress. Corticotropin-releasing hormone (CRH), through the CRH-receptor 1 (CRHR1), constitutes a key element of the stress response. However, its contribution to the effects of stress in the mPFC remains unclear.
An immunochromatographic biosensor combined with a water-swellable polymer for automatic signal generation or amplification
Contributors: Kahee Kim, Hyou-Arm Joung, Gyeo-Re Han, Min-Gon Kim
... An immunochromatographic assay (ICA) strip is one of the most widely used platforms in the field of point-of-care biosensors for the detection of various analytes in a simple, fast, and inexpensive manner. Currently, several approaches for sequential reactions in ICA platforms have improved their usability, sensitivity, and versatility. In this study, a new, simple, and low-cost approach using automatic sequential-reaction ICA strip is described. The automatic switching of a reagent pad from separation to attachment to the test membrane was achieved using a water-swellable polymer. The reagent pad was dried with an enzyme substrate for signal generation or with signal-enhancing materials. The strip design and system operation were confirmed by the characterization of the raw materials and flow analysis. We demonstrated the operation of the proposed sensor by using various chemical reaction-based assays, including metal-ion amplification, enzyme-colorimetric reaction, and enzyme-catalyzed chemiluminescence. Furthermore, by employing C-reactive protein as a model, we successfully demonstrated that the new water-swellable polymer-based ICA sensor can be utilized to detect biologically relevant analytes in human serum.
Contributors: Ming Bai, Rolf Georg Beutel, Klaus-Dieter Klass, Weiwei Zhang, Xingke Yang, Benjamin Wipfler
... A new insect species (†Alienopterus brachyelytrus Bai, Beutel, Klass, Wipfler et Zhang gen. et sp. nov.) of a new order and family is described, based on a single male embedded in Cretaceous Burmese amber (ca. 99Ma). Unusual characters are shortened forewings combined with fully developed, operational hindwings, similar as in Dermaptera, and specialized attachment pads otherwise only found in mantophasmatodeans (heelwalkers). A cladistic analysis suggests a placement as sister to Mantodea, supported by a profemoral brush and other characters. The male genitalia show the same pattern in both groups. Specialized features are the unusual flight apparatus, attachment structures adapted for locomotion on leaves, and a dense profemoral setation suitable for catching small prey. †Alienopterus was apparently able to fly and likely a predator of small arthropods in bushes or trees. An impressive radiation of Mantodea started in similar habitats at least 35Ma later in the early Cenozoic. In contrast, †Alienopterus was an evolutionary dead end in the roach–mantis transition zone.