Reshaping commensal gut microbiota in early life with amoxicillin presents with lower blood pressure
Contributors: Saroj Chakraborty
... Pediatric hypertension is recognized as an emerging global health concern. While new guidelines are developed for facilitating clinical management, the reasons for the prevalence of hypertension in children remain unknown. Genetics and environmental factors do not fully account for the growing incidence of pediatric hypertension. Because stable bacterial flora in early life are linked with health outcomes later in life, we hypothesized that reshaping of gut microbiota in early developmental stages of life affects blood pressure (BP) of pediatric subjects. To test this hypothesis, we administered amoxicillin, the most commonly prescribed pediatric antibiotic, to alter gut microbiota of young, genetically hypertensive rats (study 1) and dams during gestation and lactation to reshape microbiota of offspring (study 2). Reshaping of microbiota, with reductions in Firmicutes/Bacteriodetes ratio observed in Amoxicillin treated young rats and in dams. Amoxicillin treated rats also had lower blood pressure compared to the untreated rats. In the young rats treated with amoxicillin, the lowering effect on blood pressure persisted even after the antibiotics were discontinued. Similarly, the offspring from the dams treated with amoxicillin also showed lower systolic blood pressure compared to the control rats. Remarkably, in all cases, a decrease in BP was associated with lowering of Veillonellaceae, which are succinate-producing bacteria. Elevated plasma succinate is reported in hypertension. Accordingly, serum succinate was measured and found lower in animals treated with amoxicillin. Our results demonstrate a direct correlation between succinate-producing gut microbiota and early development of hypertension, and indicate that reshaping gut microbiota, especially by depleting succinate-producing microbiota early in life may have long-term benefits for hypertension-prone individuals.
Contributors: Zhen-zhen Zhang
... Primary giant cell tumor originating in the rib is rare, resembling giant cell tumor in the epiphyseal region of long tubular bones morphologically and immunologically. However, this lesion poses a great challenge for diagnosis due to uncommon clinical and radiological presentation and histological heterogeneity. Here we report two cases of huge giant cell tumor of ribs presented as soft tissue mass. The first patient presented as a large expanding soft tissue mass in the right thoracic cavity attached to the 4th and 5th ribs with bone destruction. The second patient showed an ill-defined soft tissue mass in the left retroperitoneum. The maximum diameter of the lesions are greater than 15cm. Apart from the typical lesion with giant cell tumor evenly distributed among the mononuclear cells, there are secondary change including multiple hemorrhage cyst formation, irregular ossification and spindle cell proliferation, which mimics malignancies. H3.3 p.G34W immunohistochemistry and H3F3A mutation detected by Sanger sequencing supported the diagnosis of giant cell tumor. Our studies emphasized radiological and histological polymorphism and the diagnostic value H3F3A mutation, with a review of other published reports to assess radiological and pathological diagnosis and outcomes in case of misdiagnosis and overtreatment.
Schistosoma mansoni infection is associated with quantitative and qualitative modifications of the mammalian intestinal microbiota
Contributors: Timothy Jenkins, Laura Peachey, Nadim Ajami, Andrew MacDonald, Michael Hsieh, Paul Brindley, Cinzia Cantacessi, Gabriel Rinaldi
... Raw 16S rRNA sequencing data from luminal contents from sections of the small (SI) and large intestines (LI) of 10 Swiss-Webster female mice (S+) that were infected with 200 S. mansoni cercariae and 10 age- and gender-matched mice that remained uninfected and were included as controls (S-). For each S+ and S- group, five mice were euthanised 28 days after infection (D28 p.i., i.e. before egg laying by sexually mature S. mansoni females had commenced). The remaining mice (n = 5 for each S+ and S-) were euthanised at day 50 p.i. (D50 p.i., corresponding with ongoing egg laying by sexually mature S. mansoni females). The experiment was carried out in duplicate and samples from both experiments (i.e. Exp. 1 & Exp. 2) pooled for further analysis. The data set includes four types of files: 1. Raw joined read files - 1 per sample (demultiplexed): e.g. Exp1_SI_S-_D50_1.fasta 2. Joined, quality filtered file containing reads from all samples and ready for OTU picking. The file was generated using Qiime: seqs.fna 3. Metadata file containing the relevant contextual information for each sample: Schisto_microbiome_Metadata.csv 4. OTU table created using Qiime including all of the taxonomic information: otu_table_mc2_w_tax_no_pynast_failures.biom
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Data for: Identifying the mechanisms that shape fungal community and metacommunity patterns in Yunnan, China.
Contributors: Kingsly Chuo Beng, Richard Corlett
... Additional results and data
Contributors: Juan_Gabriel Rueda-Bayona
... the folder contains input and setup files of the article An Alternative Method to Determine Extreme Hydrodynamic Forces with Data Limitations for Offshore Engineering
Contributors: Esteban Finol Berrueta
... raw DENV genomic data for Finol E. & Ooi EE. Iscience submision
Contributors: UnaElsLive Natra, mdgmatest5 live
... RDM - File Type Support 21May2019 ElsCustomer Apart from .u3d all files preview [ .obj / .ply / .vtk / .stl / .ent / .brk / .pdb / .pse / .mol / .mol2 / .cif / .u3d / .dcm / .nii] - .pse is not supported
Data for: Structural modifications of 2,3-indolobetulinic acid: design and synthesis of highly potent α-glucosidase inhibitors
Contributors: Elmira Khusnutdinova, Thi Tu Anh Le, Tra Nguyen Thanh, Anastasiya V. Petrova, Denis Babkov, Oxana Kazakova, Ha Nguyen Thi Thu, Cham Ba Thi
... A series of nineteen nitrogen-containing lupane triterpenoids was obtained by modification of C2, C3, C20 and C28 positions of betulonic acid and their α-glucosidase inhibiting activity was investigated. Being a leader compound from our previous study, 2,3-indolo-betulinic acid was used as the main template for different modifications at C-(28)-carboxyl group to obtain cyano-, methylcyanoethoxy-, propargyloxy- and carboxamide derivatives. 20-Oxo- and 29-hydroxy-20-oxo-30-nor-analogues of 2,3-indolo-betulinic acid were synthesized by ozonolysis of betulonic acid followed by Fischer indolization reaction. To compare the influence of the fused indole or the seven membered A-ring on the inhibitory activity, lupane A-azepanones with different substituents at C28 were synthesized. The structure-activity relationships revealed that the enzyme inhibition activity dramatically increased (up to 4730 times) when the carboxylic group of 2,3-indolo-betulinic acid was сonverted to the corresponding amide. Thus, the IC50 values for glycine amide and L-phenylalanine amides were 0.04 and 0.05 μM, respectively. This study also revealed that 2,3-indolo-platanic acid is 4.5 times more active than the parent triterpenoid with IC50 of 0.4 μM. Molecular modeling suggested that improved potency is due to additional polar interactions formed between C28 side chain and a sub-pocket of the α-glucosidase allosteric site.
Database related to the seasonal variability and trend analysis of the solar energy resource in Northeastern Brazilian region.
Contributors: Frnacisco Lima, Fernando Ramos Martins, Andre Rodrigues Gonçalves, Rodrigo Santos Costa, Enio Bueno Pereira
... Database generated produced during the evaluation study on the spatial patterns, seasonal variability and trend analysis of the surface solar irradiation in the Northeastern Brazilian region by using meteorological data acquires in automated weather stations operated by the Brazilian Institute of Meteorology from 2008 till 2015.
Data for: SUBDUCTION CONTROL ON THE CURIE ISOTHERM AROUND THE PACIFIC-NORTH AMERICA PLATE BOUNDARY IN NORTHWESTERN MEXICO (GULF OF CALIFORNIA). PRELIMINARY RESULTS.
Contributors: Oscar Campos Enriquez, erdinc oksum, Juan-Manuel Espinosa-Cardeña
... This repository contains the raw data necesay to reproduce our results. In particular, data necesary to reproduce the results shown in Figures 5a,5b, %fc, 6a, 6b, 6c, and 7a, and 7b are included, as well as instructions how to reproduce our results.