Anti-leukemic properties of aplysinopsin derivative EE-84 alone and in combination with BH3 mimetic A-1210477 (2)
Contributors: Su A Kim
... Aplysinopsins are a class of marine indole alkaloids that exhibit a wide range of biological activities such as prevention of neoplastic growth on an array of different cancer cell lines. Although both the indole and N-benzyl moieties of aplysinopsins are known to possess anti-proliferative activity against cancer cells, their mechanism of action remains unclear. Through in vitro and in vivo proliferation and viability screening of newly synthesized aplysinopsin analogs on myelogenous leukemia cell lines and zebrafish toxicity tests as well as analysis of differential toxicity in non-cancerous RPMI 1788 cells, we identified EE-84 as a promising novel drug candidate against myeloid leukemia. This indole derivative demonstrated drug-likeness in agreement with Lipinski’s rule of five and was responsible for cell cycle dysregulation in K562 cells in line with its cytostatic effect. EE-84-treated K562 cells likewise underwent morphological changes suggesting mitochondrial dysfunction. Finally, we demonstrated the synergistic cytotoxic effect of EE-84 with a BH3 mimetic, the Mcl-1 inhibitor, A-1210477, against K562 cells, highlighting the inhibition of anti-apoptotic Bcl-2 proteins as a promising therapeutic approach against myeloid leukemia in combination with EE-84.
A Novel Optimization-Based Tool to Automate Infeasible Cycle-Free Gapfilling of Genome-Scale Metabolic Models, Schroeder and Saha
Contributors: Wheaton Schroeder, Rajib Saha
... Dataset related to the paper of the same title submitted for publication in the journal iScience. The abstract is as follows.
Contributors: Ruitao Jin, Yutingw Wang, Yangpeng Liu, Chunmei Ding, Jing Xie, Jianshu Li
... The raw data of "Biomineralization and Osteogenic Differentiation Modulated by Substrate Stiffness".
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Port Data Penelitian Personalisasi Konten E-learning Berdasarkan Felder-Silverman Learning Style Model
Contributors: Jeremiah Hasudungan Sihombing
... The following data port contains the entire Data, Documents, Products, and Software generated from this research. Please use as appropriate. -JHS-2019 NB : since this research was conducted in Indonesia there are several documents that use Indonesian
Contributors: Tetsuji Okada
... DSA files of human (N to Z, by gene name) : UniProt ID is used for a protein to which no gene name is assigned.
Reshaping commensal gut microbiota in early life with amoxicillin presents with lower blood pressure
Contributors: Saroj Chakraborty
... Pediatric hypertension is recognized as an emerging global health concern. While new guidelines are developed for facilitating clinical management, the reasons for the prevalence of hypertension in children remain unknown. Genetics and environmental factors do not fully account for the growing incidence of pediatric hypertension. Because stable bacterial flora in early life are linked with health outcomes later in life, we hypothesized that reshaping of gut microbiota in early developmental stages of life affects blood pressure (BP) of pediatric subjects. To test this hypothesis, we administered amoxicillin, the most commonly prescribed pediatric antibiotic, to alter gut microbiota of young, genetically hypertensive rats (study 1) and dams during gestation and lactation to reshape microbiota of offspring (study 2). Reshaping of microbiota, with reductions in Firmicutes/Bacteriodetes ratio observed in Amoxicillin treated young rats and in dams. Amoxicillin treated rats also had lower blood pressure compared to the untreated rats. In the young rats treated with amoxicillin, the lowering effect on blood pressure persisted even after the antibiotics were discontinued. Similarly, the offspring from the dams treated with amoxicillin also showed lower systolic blood pressure compared to the control rats. Remarkably, in all cases, a decrease in BP was associated with lowering of Veillonellaceae, which are succinate-producing bacteria. Elevated plasma succinate is reported in hypertension. Accordingly, serum succinate was measured and found lower in animals treated with amoxicillin. Our results demonstrate a direct correlation between succinate-producing gut microbiota and early development of hypertension, and indicate that reshaping gut microbiota, especially by depleting succinate-producing microbiota early in life may have long-term benefits for hypertension-prone individuals.
Data for: CR6 interacting factor 1 deficiency induces premature senescence via SIRT3 inhibition in endothelial cells
Contributors: Cuk seong Kim, Seonhee Kim, Ikjun Lee, Harsha Nagar, Su-jeong Choi, Shuyu Piao, Nara Shin, Minho Shong, Dong Woon Kim, Byeong Hwa Jeon
... Raw data for submission
Research article manuscripts, data pieces, literature review, presentations etc on sharecropping and power-relations in villages in Sindh, Pakistan
Contributors: Ghulam Hussain
... Research article manuscripts based on research done in 2013-2014 on sharecropping, peasants and village culture in Sindh, Pakistan
Data for: A novel DNA repair inhibitor, diallyl disulfide (DADS), impairs DNA resection during DNA double-strand break repair by reducing the levels of Sae2 and Exo1
Contributors: Chin-Chuan Chen, Chun-Hao Feng, Chen-Hsin Kuo, Wei-Che Tseng, Tong-Hong Wang, Yann-Lii Leu, shu-huei wang
... "Fig" file is including the raw data of Fig. 1B, 1C, 2C, 3A, 3C, 3D, 3E, 3F, 4A, 4B, 5, 6A, 7A, 7D, 7E, 8A-8D and supplementary Fig. 1. “ChIP data” file is including the raw data of Fig. 2B, 4C and 4D. “Apoptosis” file is including the raw data of Fig. 6B-C. “Autophagy” file is including the raw data of Fig. 7B-C. “RNA” file is including the raw data of Fig. 3A-B.
A new method for analyzing sustainability performance of global supply chains and its application to material resources
Contributors: Livia Cabernard, Stephan Pfister, Stefanie Hellweg
... VERSION 2 Compared to version 1, version 2 runs without matlab. The file “Instructions.pdf” attached below explains how to install and use this application. The file “Explanation_method_examples.pptx” attached below illustrates the principle of the method and how to use the application with several examples. OVERALL DESCRIPTION (same as for version 1): We share here the data compiled to calculate the results presented in the study «A new method for analyzing sustainability performance of global supply chains and its application to material resources». In order to allow for the compilation of all results of interest, we provide an application. This application is based on the multi-regional-input output database EXIOBASE3 (version 3.4) and data to assess the potential environmental impacts of emissions and resource use. While the data for assessing the environmental impacts is provided here, the user must download the Exiobase data from the Exiobase website (due to copyright issues). The provided application allows to assess the cumulated upstream impacts of any sector or region on the globe without double-counting and to track these impacts upstream and downstream the global value chain. The application covers a broad set of environmental and socio-economic indicators and the timespan from 1995 to 2011. The methodology of this application is comprehensively explained in the study: «A new method for analyzing sustainability performance of global supply chains and its application to material resources» (e.g Section 2.1 gives a broad overview of the principle of the method). Link to the study: https://doi.org/10.1016/j.scitotenv.2019.04.434