5202981 results
Contributors: Kayser Oliver, Albert Sickmann, Stefan Loroch, Lukasz Marczak, Paweł Rodziewicz
Date: 2019-04-17
... Cannabinoids are terpenophenolic compounds produced by Cannabis sativa L., which accumulate in storage cavities of glandular trichomes as a part of the exudates. We investigated if tetrahydrocannabinolic acid synthase and cannabidiolic acid synthase, which are involved in the last step of cannabinoid biosynthesis, are also secreted into Cannabis trichome exudates. The exudates were collected by microsuction from storage cavities of Cannabis glandular trichomes and were subjected for proteomic and metabolomic analyses. The catalytic activity of the exudates was documented by cannabigerolic acid biotransformation studies under hydrophobic conditions. Electrophoretic separations revealed protein bands at ~65 kDa, which were further identified as tetrahydrocannabinolic acid synthase and cannabidiolic acid synthase. The accumulation of the enzymes in trichome exudates increased substantially during the flowering period in the drug-type Cannabis plants. The content of cannabinoids increased significantly after incubating hexane-diluted trichome exudates with cannabigerolic acid. In this study, we showed that Cannabis glandular trichomes secrete and accumulate cannabinoid synthases in storage cavities, and are able to convert cannabigerolic acid under hydrophobic trichome-mimicking conditions. Metabolite profiling of the exudates revealed compounds with hydrophilic, osmoprotective and amphiphilic properties, which may play a role in providing a necessary aqueous microenvironment, which enables enzyme solubility and biocatalysis under hydrophobic conditions of glandular trichomes.
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Contributors: Fabian Mueller, Marc Hanewinkel
Date: 2019-04-17
... A business process map, visualizing the wood supply chain from the tree to the mill. The process map visualizes process steps and information flows within the supply chain and outlines the opportunities of Industry 4.0 enabling the interconnection of process steps along the value chain with close to unlimited information flow and allocation in an internet of trees and services.
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Contributors: Giovanni Ludovico Montagnani
Date: 2019-04-17
... this matlab figure contains the main results of our research: a state of the art resolution gamma ray spectrum obtained with both a standard and a codoped lanthanum bromide scintillator coupled with the same sipm matrix
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Top results from Data Repository sources. Show only results like these.
Contributors: Mike Jones
Date: 2019-04-16
... Test
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Contributors: Marc Humphries, Kelly Kirsten, Terence S. McCarthy
Date: 2019-04-16
... Geochemical and diatom data for core MZ4
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Contributors: Jie Yang
Date: 2019-04-16
... Crystallization behaviors of CaO-SiO2 based slag for high Mn-high Al steels
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Contributors: UnaElsLive Natra
Date: 2019-04-16
... SMOKE TEST - second
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Contributors: Moamen Abdelkareem, Charlotte Saint-André, Maria Takacs, Gabor Papai, Corinne Crucifix, Xieyang Guo, Julio Ortiz, Albert Weixlbaumer
Date: 2019-04-15
... Denaturing polyacrylamide gels of cleavage products show that GreB accelerates RNA cleavage (left). The resulting complex is active and resumes transcription (+NTPs). Using an RNA with phosphorothioate modifications slows down the cleavage without affecting the position (right). To help the reader, cleavage_reaction_a.tif represents the raw data, while cleavage_reaction_b.tif, matches the figure in the publication (Figure S1D).
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Contributors: Takuro Matsui
Date: 2019-04-15
... a
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Contributors: Fajar Purnama, Otgontsetseg Sukhbaatar, Lodoiravsal Choimaa, Tsuyoshi Usagawa
Date: 2019-04-15
... The data are based from mouse tracking experiments at https://md.hicc.cs.kumamoto-u.ac.jp. 1. Filenames with "P2P" contains network packet captured of one click event submitted from the client to the server. The filenames also have the number of variables included such as name, email, date, current URL, etc, refer to "Summary_of_Data.ods". 2. Filename with "Local" contains resource cost and mouse tracking data of five users attempting a quiz session on the server via the Internet on 25 December 2018. 3. Filename with "Implementation" contains resource cost of a mouse tracking implementation of two quiz session attempted by twenty-two students on each session from School of Engineering and Applied Sciences, National University of Mongolia to the server at Human Interface and Cyber Communication laboratory, Kumamoto University on 03 January 2019. Unfortunately, the mouse tracking data is not open for a limited amount of time.
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