Contributors: Pablo Ferrada
... -Folder MATLAB files contains *mat files, which is data to start the described code in the data in brief manuscript. -Folder Photographs, Flasks 1-4 contains pictures of rising bubbles in a flask. -Folder Videos, Flasks 1-4 contains videos of the rising bubbles in a flask.
Contributors: Massimo Salvi
... This repository contains the FAST algorithm graphical user interface and some sample image used in the following work: - Salvi M., Cerrato V., Buffo A., and Molinari F., "Automated Segmentation of Brain Cells for Clonal Analyses in Fluorescence Microscopy Images", J Neurosci Methods 2019 (DOI: 10.1016/j.jneumeth.2019.108348) ABSTRACT The understanding of how cell diversity within and across distinct brain regions is ontogenetically achieved is a pivotal topic in neuroscience. Clonal analyses based on multicolor cell labeling represent a powerful tool to tackle this issue and disclose lineage relationships, but produce enormous sets of fluorescence images, leading to time consuming analyses that may be biased by the operator’s subjectivity. Thus, time-efficient automated software are needed to analyze images easily, accurately and without subjective bias. In this paper, we present a fully automated method, named FAST (‘Fluorescent cell Analysis Segmentation Tool’), for the segmentation of neural cells labeled by multicolor combinations of fluorophores and for their classification into clones. The proposed method was tested on 77 high-magnification fluorescence images of adult mouse cerebellar tissues acquired using a confocal microscope. Automatic results were compared with manual annotations and two open-source software designed for cell detection in microscopic imaging. The algorithm showed very good performance in the cellular detection and in the assignment of the clonal identity. To the best of our knowledge, FAST is the first fully automated technique for the analysis of cellular clones based on combinatorial expression of fluorescent proteins. The proposed approach allows to perform clonal analyses easily, accurately and objectively, overcoming those biases and errors that may result from manual annotations. Moreover, it can be broadly applied to the quantification and colocalization within cells of fluorescent markers, therefore representing a versatile and powerful tool for automated quantitative analyses in fluorescence microscopy.
Improve the interfacial adhesion, corrosion resistance and combustion properties of aluminum powder by modification of nickel and dopamine
Contributors: Li Liang, Xiaode Guo, Xin Liao, Zhipeng Chang
... To improve the interface incompatibility, poor corrosion resistance, and limited combustion efficiency of aluminum powder for propellant applications. The surface of the naked aluminum was coated with a layer of dense nickel of about 100 nm by replacement method. A polydopamine film was then deposited by self-polymerization. The double-layer structure and chemical composition were investigated. The polydopamine content of composite particles was 2.3% by thermal analysis. And its heat release around 1050 C reached 4137 J g-1, which was much higher than that of aluminum of 1644 J g-1. The surface energies was used to calculate the theoretical adhesion work of aluminum and composite particles to propellant binder. The adhesion work after modification was increased from 62.33 mN m-1 to 74.89 mN m-1. No matter in the weak acid/alkaline or neutral environment, the composite particles exhibited excellent stability at 50 C. In addition, the combustion test revealed that the composite particles had higher combustion efficiency, smaller condensed phase product size, and stronger ignition ability. All the conclusions indicate after modification by nickel and dopamine, the comprehensive performance of aluminum is improved, and it may be used as an energy component in propellants or other energetic fields.
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Contributors: UnaElsLive Natra
... MD-2831 Update Java version in the Docker containers
Supplementary Information for: "Simulation of convective moistening of extratropical lower stratosphere using a numerical weather prediction model"
Contributors: Zhipeng Qu
... Video SI.1: the animation of 1 km simulation on a chosen cross-section during the convection event from 18:40 to 20:40 UTC, 25 Aug 2013. 1st panel: temperature (colour) and isentropic lines (thin white lines); 2nd panel: ice water content in logarithm scale; 3rd panel: water vapor content in ppmv (colour saturated at 20 ppmv); 4th panel: vertical wind speed (m s-1). Video SI.2: water vapor content in ppmv at the altitude of 16.5 km from 18:00 to 23:00 UTC (5 hours) within the evaluation Domain A. Figure SI.1: the transported water vapor in the simulation domain of 0.25 km (green box in Fig. 1) from 1800 to 19:00 UTC 25 Aug. The vertical advection of 10 km simulation is decomposed into two parts: grid scale advection (light blue bar) and sub-grid scale advection from KFC parameterization (darker blue bar). For the results presented in Fig. SI.1, the evaluation is performed for the simulated domain of 0.25 km grid-spacing (green box in Fig. 1) for the first hour of the evaluation time window (from 18:00 to 19:00 UTC 25 Aug for the three high resolution models, from 21:30 to 22:30 UTC 25 Aug for 10 km simulation). The tropopause levels are defined using the mean laps rate <2˚C km-1. The use of limited time window is due to the fact that the storm moves quickly to the outside of the simulation domain. Figure SI.1 shows the mass transport budget for the 4 simulations with different horizontal resolutions, we found that the direct transport of water vapor of 0.25 km simulation is higher than all the other simulations. Although the contributions from ice sublimation and turbulent mixing of 0.25 km simulation are the lowest. The contribution from ice sublimation is negative which suggests that vapor deposition rate is very high in the convection updrafts and exceeds that of ice sublimation. Although the results presented here should be viewed with caution due to the short evaluation period (1 hour), it corroborates the finding that higher resolution model tends to have a stronger direct vertical transport of water vapor and a weaker contribution from the ice sublimation.
Contributors: James Burke
... Raw images for Supplemental Figures S4-S7
Formation of artifact-hydride in Zr-containing amorphous alloys during sample preparation and atom probe tomography
Contributors: chetarpa yipyintum
... Hydride formation in Zr-containing amorphous alloys as an experimental artifact was investigated utilizing atom probe tomography, transmission electron microscopy, and focused ion beam with normal and cryogenic conditions. The amount of hydrogen existing in the atom probe specimens decreased significantly by utilizing focused ion beam milling under cryogenic condition. Also, the formation of hydride was confirmed by diffraction pattern analysis in the remaining tip of the specimen after the atom probe tomography experiment. With those collected pieces of evidence, sources of hydrogen in the atom probe tomography were discussed.
Contributors: Anders Thomsen, Morten Kristiansen, Ewa Kristiansen, Benny Endelt
... The data describes the measurement of a v-bend shape formed during multi-scan laser forming. The purpose of the measurements was to determine the dynamic response during laser forming of a v-bend. A measurement scanner was used to measure the height of a line perpendicular to the heating scan line of a laser during laser forming. In order to estimate a surface, 105 samples were made with identical settings with the measurement scanner moved along the heating scan line between samples. A total of 21 positions along the heating scan line were measured. Each position was measured using 5 samples. Due to a memory problem, the measurement scanner could only measure about 3.12 seconds at a time. The measurement scanner is started slightly before each heating scan line starts. Furthermore, each heating scan line is split into its own text file in the data set. This data set contains 21 folders, one for each position of the measurement scanner along the heating scan line. The folders are named as 'ymm', where y is the distance from the trailing edge of the heating scan line, '_' is used instead of decimals here. Each folder contains 30 text files, 6 for each of the samples used, structured as (x, y, z, t). Each file is named as 'sample_i_plate_j_scannumber_k.txt', where i is the sample number (1-5) at this position, j is the plate number (1 or 2), k is the scan number (1-6). Scan number 6 does not contain any heating, but is set as a final measurement of about 60 seconds after forming. Warning: The unzipped data fill 51.6 GB
Contributors: Yujun Lee
... Foot reﬂexotherapy was effective on auditory recuperation of infants with SNHL
Contributors: songjia tang
... Modified single-hair follicular unit grafting to aesthetically restore eyelashes in Asians