Contributors: Griffin Walker
... Griffin Walker's demo, released in 2004. It's not mastered, please be kind.
Supplemental material for JAAD publication titled - Palm reading and water divining: A cross-sectional study of the accuracy of palmar hyper-linearity and trans-epidermal water loss to identify individuals with a filaggrin gene null mutation.
Contributors: Adrian Lowe
... Supplemental material for publication titled - "Palm reading and water divining: A cross-sectional study of the accuracy of palmar hyper-linearity and trans-epidermal water loss to identify individuals with a filaggrin gene null mutation" to be published in Journal of the American Academy of Dermatology
Contributors: Heather Coker, Neil brockdorff, Guifeng Wei, Benoit Moindrot, Tatyana Nesterova, Shabaz Mohammed
... X chromosome inactivation in mammals is regulated by the non-coding (nc) RNA, Xist, which represses the chromosome from which it is transcribed. High levels of the N6-methyladenosine (m6A) RNA modification occur within Xist exon I, close to the 5’ end of the transcript, and also further 3’, in Xist exon VII. The m6A modification is catalysed by the METTL3/14 complex that is directed to specific targets, including Xist, by the RNA binding protein RBM15/15B. m6A modification of Xist RNA has been reported to be important for Xist–mediated gene silencing. We use CRISPR/Cas9 mediated mutagenesis to delete sequences around the 5’ m6A region in interspecific XX mouse embryonic stem cells (mESC). Following induction of Xist RNA expression we assay chromosome silencing using allelic RNA-seq and Xist m6A patterns using m6A-seq. Additionally we use Xist RNA FISH to analyse the effect of deleting the 5’ m6A region on the function of the endogenous Xist promoter. Purifying epitope tagged RBM15, we employ MS/MS analysis to define the RBM15 interactome in mESCs. We show that a deletion encompassing the entire Xist 5’ m6A region results in a modest reduction in Xist-mediated silencing, and that the 5’ m6A region overlaps essential DNA elements required for activation of the endogenous Xist promoter. Deletion of the Xist A-repeat, to which RBM15 binds, entirely abolishes deposition of m6A in the Xist 5’ m6A region without affecting the modification in exon VII. We show that in mESCs RBM15 interacts with the m6A complex, the SETD1B histone modifying complex, and several proteins linked to RNA metabolism
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Contributors: Eric Schniter, Shane J. Macfarlan, Juan J. Garcia
... In 2017 and 2018, seventy-one individuals (33 females and 38 males) from 40 ranches across three Choyero communities in the Sierra de la Giganta of Baja California Sur, Mexico were presented an ethnobiological knowledge assessment task. As a first step of the task, individuals took a vision acuity test using “tumbling E” eye charts presented on a laptop computer (see TumblingE_EyeChart.pptx), a robust and easy to use diagnostic tool that is practical for populations with innumerate or analphabetic participants (Messina et al. 2006). From this test we derived a visual acuity score [1,…11]. A higher score indicates better visual acuity. The ethnobiological knowledge assessment task presented a sequence of 137 slides on a laptop computer featuring images of 87 plants and 50 animals (see EthnobiologicalKnowledgeAssessmentTool.pptx). The order of items presented was varied among participants. Assessments of ethnobiological knowledge were conducted in Spanish in the privacy of individual homes and occurred as part of a larger set of household interviews regarding ranching demography and lifestyles. These household interviews additionally informed us of individuals’ educational achievement, their ranch affiliation, and community membership. For each of 137 plant and animal images presented in sequence, the researcher (ES) asked the participant whether they recognized the item in the image. If the participant answered affirmatively, the researcher asked the follow-up question about what the item is called. Responses were recorded and coded “correctly named” if matching locally appropriate culturally correct names used for species identification (see Macfarlan et al., 2020 http://dx.doi.org/10.17632/kjds8jztzv.1). Here we provide raw data generated by seventy-one participants (33 females and 38 male). We also include a codebook that explains data types and meanings and we provide the .pptx files for tumbling E eye charts and the ethnobiological knowledge assessment task used to generate the data in this survey.
Astrocyte-derived extracellular vesicles enhance the electrophysiological function and cell survival of human cortical neurons in vitro
Contributors: Changho Chun, Alec Smith, Deok-Ho Kim
... The immunostained image analysis of neurons created, NTA analysis of obtained EVs, image-based demonstration of EV-uptake by neurons, FACS analysis to quantify apoptotic behavior of cultures of interest, beta-galactosidase result to explain cell senescence status in each group, patch clamp experiment result to demonstrate the electrophusiological effect of astroglial EV treatment.
Data for: Development and validation of a rapid LC-MS/MS method for the quantification of mycosporines and mycosporine-like amino acids (MAAs) from cyanobacteria
Contributors: Ernani Pinto, Rios Jacinavicius, Paul F. Long, Lívia Soman, Vanessa Geraldes
... Validation Data UV-Induction experiments data
Data for: Quantitative analysis of the aqueous humor metabolites and changes in glucose metabolism in patients with central retinal vein occlusion using UHPLC-MS/MS
Contributors: Guoge Han, Meiqin He, Pinghui Wei, He Teng
... Standard Calibration Curve
Contributors: Zhouli Cheng
... source data of TET2-ZSCAN project.
Contributors: Hary Murcahyanto
... Data ini adalh data hasil wawancara penelitian alat Musik Trasisional Lombok "Selober" yang menjadi bahan kajian utama pada penelitian ini,