Reshaping commensal gut microbiota in early life with amoxicillin presents with lower blood pressure
Contributors: Saroj Chakraborty
... Pediatric hypertension is recognized as an emerging global health concern. While new guidelines are developed for facilitating clinical management, the reasons for the prevalence of hypertension in children remain unknown. Genetics and environmental factors do not fully account for the growing incidence of pediatric hypertension. Because stable bacterial flora in early life are linked with health outcomes later in life, we hypothesized that reshaping of gut microbiota in early developmental stages of life affects blood pressure (BP) of pediatric subjects. To test this hypothesis, we administered amoxicillin, the most commonly prescribed pediatric antibiotic, to alter gut microbiota of young, genetically hypertensive rats (study 1) and dams during gestation and lactation to reshape microbiota of offspring (study 2). Reshaping of microbiota, with reductions in Firmicutes/Bacteriodetes ratio observed in Amoxicillin treated young rats and in dams. Amoxicillin treated rats also had lower blood pressure compared to the untreated rats. In the young rats treated with amoxicillin, the lowering effect on blood pressure persisted even after the antibiotics were discontinued. Similarly, the offspring from the dams treated with amoxicillin also showed lower systolic blood pressure compared to the control rats. Remarkably, in all cases, a decrease in BP was associated with lowering of Veillonellaceae, which are succinate-producing bacteria. Elevated plasma succinate is reported in hypertension. Accordingly, serum succinate was measured and found lower in animals treated with amoxicillin. Our results demonstrate a direct correlation between succinate-producing gut microbiota and early development of hypertension, and indicate that reshaping gut microbiota, especially by depleting succinate-producing microbiota early in life may have long-term benefits for hypertension-prone individuals.
Contributors: Matthias Sinnesael, Sietske J. Batenburg, Christian Zeeden, Jiri Laurin, Damien Pas, Linda Hinnov, Johannes Monkenbusch, Maximilian Vahlenkamp, Mingsong Li, Sébastien Wouters
... Supplementary materials for Submission: Sinnesael et al., Earth-Science Reviews "The Cyclostratigraphy Intercomparison Project (CIP): consistency, merits and pitfalls ".
Bridging sensory evaluation and consumer research for strategic leafy Brassica (Brassica oleracea) improvement
Contributors: Hannah Swegarden, Phillip Griffiths, Alina Stelick, Robin Dando
... Research data uploaded here provided the foundation for analysis of Qualitative Multivariate Analysis (QMA_FocusGroupObservations), descriptive analysis (QDA_Sessions123 and ConsumerClusterDemo_Kale2019), and consumer testing (ConsumerKale_FinalData). These data have been cleaned and formatted from raw datasets; they are ready for downstream analysis. Participant journal entries and raw data are available upon request.
Top results from Data Repository sources. Show only results like these.
Contributors: Chunli Dai
... Here are the results in a paper entitled "Characterization of the 2008 phreatomagmatic eruption of Okmok from ArcticDEM and InSAR: deposition, erosion, and deformation" submitted to JGR Solid Earth in 2019. It includes the 2-m resolution surface elevation change of the 2008 Okmok eruption (Fig. 2a in the paper) and the 2-m resolution post-eruptive elevation change rate map (Fig. 3), as well as the corresponding uncertainties (Fig. S3). It also includes the boundary of the proximal deposit field classified using a minimum elevation increase of 2 m, the boundary of large slope failure, and the shorelines of two lakes (Figs. 2a, S5, and S6) at different acquisition times. The GeoTIFF files can be viewed in free and open-source software QGIS, in Google Earth, or by Matlab using code https://github.com/ihowat/setsm_postprocessing/blob/master/readGeotiff.m. The shapefiles can be viewed in QGIS and Google Earth.
Contributors: Soner Buytoz
... It's the raw information of my work.
Contributors: Solam Lee
... AloNet Author: Solam Lee (firstname.lastname@example.org) AloNet is a convolutional neural network based on U-Net that can identify the hair loss and the scalp area by analying clinical photograph. This model was developed for the automated calculation of the Severity of Alopecia Tools (SALT) score in assessment of patients with alopecia areata. This repository posts the Mendeley Supplementary Materials, the program code, and the relevant data used in the paper titled "Clinically Applicable Deep Learning Framework for Measurement of the Severity of Alopecia Tool Score in Patients with Alopecia Areata". Along with the programs in the "/Program/" directory, a total of 2716 pixelwise annotations used for train the hair loss identifier (mask) and the hair loss identifier (target) could be find in the "/Data/" directory. However, please note that the clinical photograph of the patients could not be made publicly available because of strict privacy regulation. Before using AloNet program with your dataset, you should convert your dataset into numpy files. One clinical photograph (saved in .jpg with RGB format) need each annotation for the scalp area (saved in .gif with black&white color) and the hair loss (saved in .gif with black&white color), respectively. Please make sure that they have same image size each other, or the conversion will fail. We are now currently working on several postprocessing algorithms for AloNet to be available for general use. The Flask web application and its code will be made available publicly when the program is ready to use.
Data/Software for "Presynaptic Mitochondria Volume and Abundance Increase During Development of a High-Fidelity Synapse"
Contributors: Connon I. Thomas, Christian Keine, Satoko Okayama, Rachel Satterfield, Morgan Musgrove, Debbie Guerrero-Given, Naomi Kamasawa, Samuel M. Young, Jr.
... Contains data and software from the publication: "Presynaptic Mitochondria Volume and Abundance Increase During Development of a High-Fidelity Synapse" published in the The Journal of Neuroscience (https://doi.org/10.1523/JNEUROSCI.0363-19.2019). The preprint to this data set has been published on bioRxiv (https://doi.org/10.1101/689653). In this study, we created a helper-dependent adenoviral vector (HdAd) to co-express cytoplasmic EGFP and a genetically encoded peroxidase marker (mito-APEX2) at the calyx of Held, an excellent model for deciphering regulatory mechanisms of presynaptic function. ABSTRACT: The calyx of Held, a large glutamatergic presynaptic terminal in the auditory brainstem undergoes developmental changes to support the high action-potential firing rates required for auditory information encoding. In addition, calyx terminals are morphologically diverse which impacts vesicle release properties and synaptic plasticity. Mitochondria influence synaptic plasticity through calcium buffering and are crucial for providing the energy required for synaptic transmission. Therefore, it has been postulated that mitochondrial levels increase during development and contribute to the morphological-functional diversity in the mature calyx. However, the developmental profile of mitochondrial volumes and subsynaptic distribution at the calyx of Held remains unclear. To provide insight on this, we developed a helper-dependent adenoviral vector (HdAd) that expresses the genetically encoded peroxidase marker for mitochondria, mito-APEX2, at the mouse calyx of Held. We developed protocols to detect labeled mitochondria for use with serial block face scanning electron microscopy to carry out semi-automated segmentation of mitochondria, high-throughput whole terminal reconstruction and presynaptic ultrastructure in mice of either sex. Subsequently, we measured mitochondrial volumes and subsynaptic distributions at the immature postnatal day 7 (P7) and the mature (P21) calyx. We found an increase of mitochondria volumes in terminals and axons from P7 to P21 but did not observe differences between stalk and swelling subcompartments in the mature calyx. Based on these findings, we propose that mitochondrial volumes and synaptic localization developmentally increase to support high firing rates required in the initial stages of auditory information processing. Data are sorted by the figures they appear in. Media (movies and 3D models) and custom-written software are located in separate folders.
The relationship between regular sinusoidal waves in the tissue at lung-related acupuncture points and the respiration pacesetter mechanism
Contributors: Fletcher Kovich
... This data compliments a paper entitled: The relationship between regular sinusoidal waves in the tissue at lung-related acupuncture points and the respiration pacesetter mechanism. The paper fully describes and analyses the data. Supplemental to that paper, the following document analyses aspects of the “pace signals” to rule out the possibility that the pace signals are artefact. http://www.curiouspages.com/research/01.pdf At the time of writing, the main paper is still in production. Please see the links at the end of this dataset, which will be updated once the paper is published. A preprint draft of the main paper is available at https://www.preprints.org/manuscript/201910.0201/v2
Oxidative stress induced by the tetrahydrobenzimidazole TMQ0153 modulates crosstalk between apoptosis, autophagy and necroptosis in chronic myeloid leukemia (Part 3 - Figure 4 (b), Figure 5, 6, 7, Supplementary 1-5)
Contributors: Sungmi Song
... Aims: We demonstrate the capacity of the cytotoxic synthetic tetrahydrobenzimidazole (TMQ) hydroquinone (HQ) derivative TMQ0153 to induce reactive oxygen species (ROS) and mediate differential cell death modalities in chronic myeloid leukemia (CML). Results: Results showed that concentrations of TMQ0153 30 µM lead to ROS-mediated necrostatin-sensitive necroptosis. Moreover, TMQ0153 allowed us to elucidate the interplay of ROS-mediated cell death and cell stress/survival mechanisms as this compound triggers protective autophagy in response to metabolic stress in CML cells. Importantly, the modulation of cell stress prior to TMQ0153-induced cell death enhances the exposure of “find-me”/“eat-me” signals considered as hallmarks of immunogenic cell death (ICD), altogether providing a pro-oxidant therapeutic strategy against CML. Innovation: We suggest here a pro-oxidant anti-CML therapy leading to differential cell death modalities in both imatinib-sensitive and -resistant CML cell types. Moreover, we elucidated the interplay between apoptosis, autophagy and controlled necrosis induced by TMQ0153 leading to disruption of mitochondrial homeostasis. Conclusion: Our findings indicate that TMQ0153-induced ROS act as a rheostat determining the onset of apoptotic- or autophagy-related controlled necrotic cell death in CML.
Data for: Substantially enhanced varistor properties and dielectric response in (Zn2+, Sn4+) co-doped CaCu3Ti4O12 ceramics
Contributors: Prasit Thongbai
... Raw data for all figures in this paper.