Reshaping commensal gut microbiota in early life with amoxicillin presents with lower blood pressure
Contributors: Saroj Chakraborty
... Pediatric hypertension is recognized as an emerging global health concern. While new guidelines are developed for facilitating clinical management, the reasons for the prevalence of hypertension in children remain unknown. Genetics and environmental factors do not fully account for the growing incidence of pediatric hypertension. Because stable bacterial flora in early life are linked with health outcomes later in life, we hypothesized that reshaping of gut microbiota in early developmental stages of life affects blood pressure (BP) of pediatric subjects. To test this hypothesis, we administered amoxicillin, the most commonly prescribed pediatric antibiotic, to alter gut microbiota of young, genetically hypertensive rats (study 1) and dams during gestation and lactation to reshape microbiota of offspring (study 2). Reshaping of microbiota, with reductions in Firmicutes/Bacteriodetes ratio observed in Amoxicillin treated young rats and in dams. Amoxicillin treated rats also had lower blood pressure compared to the untreated rats. In the young rats treated with amoxicillin, the lowering effect on blood pressure persisted even after the antibiotics were discontinued. Similarly, the offspring from the dams treated with amoxicillin also showed lower systolic blood pressure compared to the control rats. Remarkably, in all cases, a decrease in BP was associated with lowering of Veillonellaceae, which are succinate-producing bacteria. Elevated plasma succinate is reported in hypertension. Accordingly, serum succinate was measured and found lower in animals treated with amoxicillin. Our results demonstrate a direct correlation between succinate-producing gut microbiota and early development of hypertension, and indicate that reshaping gut microbiota, especially by depleting succinate-producing microbiota early in life may have long-term benefits for hypertension-prone individuals.
Contributors: Nikola Tošić, Adam Knaack, Yahya Kurama
... This dataset contains supporting documentation explaining the use of time-dependent concrete material models TDConcrete, TDConcreteEXP, TDConcreteMC, and TDConcreteMC10NL in OpenSees. The dataset contains a manual explaining the use and features of the models, an Excel table for calculating model input parameters and example files using the material model TDConcreteMC10NL on a specific example.
Contributors: Alon Poleg-Polsky
... Simulation of neuronal processing of noise-corrupted inputs NEURON simulation (http://neuron.yale.edu/neuron)
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Oxidative stress induced by the tetrahydrobenzimidazole TMQ0153 modulates crosstalk between apoptosis, autophagy and necroptosis in chronic myeloid leukemia (Part 3 - Figure 4 (b), Figure 5, 6, 7, Supplementary 1-5)
Contributors: Sungmi Song
... Aims: We demonstrate the capacity of the cytotoxic synthetic tetrahydrobenzimidazole (TMQ) hydroquinone (HQ) derivative TMQ0153 to induce reactive oxygen species (ROS) and mediate differential cell death modalities in chronic myeloid leukemia (CML). Results: Results showed that concentrations of TMQ0153 30 µM lead to ROS-mediated necrostatin-sensitive necroptosis. Moreover, TMQ0153 allowed us to elucidate the interplay of ROS-mediated cell death and cell stress/survival mechanisms as this compound triggers protective autophagy in response to metabolic stress in CML cells. Importantly, the modulation of cell stress prior to TMQ0153-induced cell death enhances the exposure of “find-me”/“eat-me” signals considered as hallmarks of immunogenic cell death (ICD), altogether providing a pro-oxidant therapeutic strategy against CML. Innovation: We suggest here a pro-oxidant anti-CML therapy leading to differential cell death modalities in both imatinib-sensitive and -resistant CML cell types. Moreover, we elucidated the interplay between apoptosis, autophagy and controlled necrosis induced by TMQ0153 leading to disruption of mitochondrial homeostasis. Conclusion: Our findings indicate that TMQ0153-induced ROS act as a rheostat determining the onset of apoptotic- or autophagy-related controlled necrotic cell death in CML.
Contributors: Laura Newsome
... Data associated with manuscript 'Manganese and cobalt redox cycling in laterites; biogeochemical and bioprocessing implications'
Contributors: M. Sana Ullah Sahar
... This archive contains firmware of the nerve stretcher as well as necessary files to develop PCB for the device. This project is actively being maintained at GitHub. The updated versions of these scripts can be downloaded any time from our GitHub repository (https://github.com/msanaullahsahar/nestv2). If you find any issue/problem/error in these scripts please do not hesitate to report at (https://github.com/msanaullahsahar/nestv2/issues).
Contributors: Jorge Bravo-Martinez
... The device is to automate Western Blot
Contributors: Qiankun Liu, Jingang Jiang, Changwei Jing, Zhong Liu, Jiaguo Qi
... In this paper, a new, alternative, multi-scale, multi-pollution source waste load allocation (WLA) system was developed, with a goal to produce optimal, fair quota allocations at multiple scales. The new WLA system integrates multi-constrained environmental Gini coefficients (EGCs) and Delphi-analytic hierarchy process (Delphi-AHP) optimization models to achieve the stated goal. This dataset consists of the raw data and the source code of models (The multi-constrained environmental Gini coefficients and Delphi-analytic hierarchy process optimization models). The source code of the multi-constrained EGCs and Delphi-AHP models was used to run the program in MATLAB environment to allocate waste load reduction quotas at both the regional scale and the site-specific scale with multiple pollution sources. The raw data mainly consists of the following two parts: (1) The shp files of various geographic information data, which was used to depicture the administrative divisions, pollution source distribution, geographical characteristics and patterns of Xian-jiang watershed; (2) The basic data includes the statistical yearbook data of villages and towns in Ningbo city, the various indicator data using to calculate the weights at criteria level and decision-making level, the contribution coefficients, and the EGC values of the three pollutants. On the basis of these data, a new, alternative, multi-scale, multi-sector optimal WLA framework was developed. The new scheme provides decision-makers critical information (i.e., the best compromise solutions of WLA) and practical guidance as they address the related water pollution control. The results, in comparison with existing practices by the local governments, suggested that the pollution discharge quota at regional scale is much fairer than the existing WLA and, even have some environmental economic benefits at pollutant source scale after optimal WLA. Some important conclusions had been found: 1) Reductions and proportions of pollutants at regional scale are significantly associated with the region’s actual socioeconomic development modes. 2）There are certain characteristics that high-reduced pollution sources tend to share (which are listed in the article). The sources with the above features should be the top priorities in the reduction of removals. 3）Most previous studies reported primarily on the WLA of removals among point sources pollution. Conversely, we found that the industrial pollution source should be the last option for reduction from an environmental-economic benefit perspective. Instead, the often overlooked types, such as agricultural non-point source and domestic sources, deserve more attention, especially in extensive rural areas.
Contributors: UnaElsLive Natra
... MD-2831 Update Java version in the Docker containers
Jyoti and Haese, 2019, "The transition from face dissolution to wormhole formation in a heterogeneous limestone based on the distribution of local dissolution rates"
Contributors: Apoorv Jyoti, Ralf R. Haese
... The findings from the dataset presented here are described in the paper titled "The transition from face dissolution to wormhole formation in a heterogeneous limestone based on the distribution of local dissolution rates" by Apoorv Jyoti and Ralf. R. Haese from the Peter Cook Center for CCS Research at the University of Melbourne. A multicomponent reactive transport model at the pore-scale in combination with a flow-through experiment with intermittent high-resolution imaging of the pore network geometry is used to better understand the formation of dissolution patterns in porous limestone. The 3D images of the pore network were used for reactive-transport simulations to derive the distribution of local dissolution rates. The model was validated through the comparison of experimental and integrated calcite dissolution rates derived from simulations. The image-based change in pore geometry was limited to less than 3 mm from the inflow of acid due to the rapid acid neutralization from calcite dissolution. The dataset consists of three parts: 1) Raw image data from the micro-CT scanner, 2) Chemical analysis of the effluent presented in an excel file, 3) The three reactive transport models at T0, T2, and T4, consisting of the input files for COMSOL and PhreeqC.