Reshaping commensal gut microbiota in early life with amoxicillin presents with lower blood pressure
Contributors: Saroj Chakraborty
... Pediatric hypertension is recognized as an emerging global health concern. While new guidelines are developed for facilitating clinical management, the reasons for the prevalence of hypertension in children remain unknown. Genetics and environmental factors do not fully account for the growing incidence of pediatric hypertension. Because stable bacterial flora in early life are linked with health outcomes later in life, we hypothesized that reshaping of gut microbiota in early developmental stages of life affects blood pressure (BP) of pediatric subjects. To test this hypothesis, we administered amoxicillin, the most commonly prescribed pediatric antibiotic, to alter gut microbiota of young, genetically hypertensive rats (study 1) and dams during gestation and lactation to reshape microbiota of offspring (study 2). Reshaping of microbiota, with reductions in Firmicutes/Bacteriodetes ratio observed in Amoxicillin treated young rats and in dams. Amoxicillin treated rats also had lower blood pressure compared to the untreated rats. In the young rats treated with amoxicillin, the lowering effect on blood pressure persisted even after the antibiotics were discontinued. Similarly, the offspring from the dams treated with amoxicillin also showed lower systolic blood pressure compared to the control rats. Remarkably, in all cases, a decrease in BP was associated with lowering of Veillonellaceae, which are succinate-producing bacteria. Elevated plasma succinate is reported in hypertension. Accordingly, serum succinate was measured and found lower in animals treated with amoxicillin. Our results demonstrate a direct correlation between succinate-producing gut microbiota and early development of hypertension, and indicate that reshaping gut microbiota, especially by depleting succinate-producing microbiota early in life may have long-term benefits for hypertension-prone individuals.
Genome-wide identification and transcriptional profiling of small heat shock protein gene family under diverse abiotic stress conditions in Sorghum bicolor (L.)
Contributors: Kavi Kishor Polavarapu, anilkumar somanaboina, Manohar Rao D, Sudhkar Reddy Palakolanu, Rajasheker G, Anuj Kumar, nagaraju marka
... The small heat shock proteins (sHsps/Hsp20s) are the molecular chaperones that maintain proper folding, trafficking and disaggregation of proteins under diverse abiotic stress conditions. In the present investigation, a genome-wide scan revealed the presence of a total of 47 sHsps in Sorghum bicolor (SbsHsps), distributed across 10 subfamilies, the major subfamily being P (plastid) group with 17 genes. Chromosomes 1 and 3 appear as the hot spot regions for SbsHsps, and majority of them were found acidic, hydrophilic, unstable and intron less. Interestingly, promoter analysis indicated that they are associated with both biotic and abiotic stresses, as well as plant development. Sorghum sHsps exhibited 15 paralogous and 20 orthologous duplications. Expression analysis of 15 genes selected from different subfamilies showed high transcript levels in roots and leaves implying that they are likely to participate in the developmental processes. SbsHsp genes were highly induced by diverse abiotic stresses inferring their critical role in mediating the environmental stress responses. Gene expression data revealed that SbsHsp-02 is a candidate gene expressed in all the tissues under varied stress conditions tested. Our results contribute to the understanding of the complexity of SbsHsp genes and help to analyse them further for functional validation.
Oxidative stress induced by the tetrahydrobenzimidazole TMQ0153 modulates crosstalk between apoptosis, autophagy and necroptosis in chronic myeloid leukemia (Part 3 - Figure 4 (b), Figure 5, 6, 7, Supplementary 1-5)
Contributors: Sungmi Song
... Aims: We demonstrate the capacity of the cytotoxic synthetic tetrahydrobenzimidazole (TMQ) hydroquinone (HQ) derivative TMQ0153 to induce reactive oxygen species (ROS) and mediate differential cell death modalities in chronic myeloid leukemia (CML). Results: Results showed that concentrations of TMQ0153 30 µM lead to ROS-mediated necrostatin-sensitive necroptosis. Moreover, TMQ0153 allowed us to elucidate the interplay of ROS-mediated cell death and cell stress/survival mechanisms as this compound triggers protective autophagy in response to metabolic stress in CML cells. Importantly, the modulation of cell stress prior to TMQ0153-induced cell death enhances the exposure of “find-me”/“eat-me” signals considered as hallmarks of immunogenic cell death (ICD), altogether providing a pro-oxidant therapeutic strategy against CML. Innovation: We suggest here a pro-oxidant anti-CML therapy leading to differential cell death modalities in both imatinib-sensitive and -resistant CML cell types. Moreover, we elucidated the interplay between apoptosis, autophagy and controlled necrosis induced by TMQ0153 leading to disruption of mitochondrial homeostasis. Conclusion: Our findings indicate that TMQ0153-induced ROS act as a rheostat determining the onset of apoptotic- or autophagy-related controlled necrotic cell death in CML.
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Contributors: Isabel Correia, Ceyda Acilan
... Rawdata for the Data in Brief paper: "EXPERIMENTAL DATA ON NOVEL FE(III)-COMPLEXES CONTAINING PHENANTHROLINE DERIVATIVES FOR THEIR ANTICANCER PROPERTIES"
Port Data Penelitian Personalisasi Konten E-learning Berdasarkan Felder-Silverman Learning Style Model
Contributors: Jeremiah Hasudungan Sihombing
... Port data berikut berisi keseluruhan Data, Dokumen , Produk, dan Perangkat Lunak yang dihasilkan dari penelitian ini. Mohon untuk digunakan sebagai mana mestinya. -JHS-2019
Contributors: Ines Diaz-Laviada, Belen Sanchez, Alicia Bort, Pedro Mateos, Nieves Rodríguez-Henche
... Data used to generate figures of the paper “Combination of the natural product capsaicin and docetaxel synergistically kills human prostate cancer cells through the metabolic regulator AMP-activated kinase” Sánchez BG, Bort A, Mateos-Gómez PA, Rodríguez-Henche N, Díaz-Laviada I. Cancer Cell Int. 2019 Mar 8;19:54. doi: 10.1186/s12935-019-0769-2. eCollection 2019. In this study we demonstrate that the spicy ingredient of peepers, capsaicin, sensitizes prostate cancer cell to docetaxel. The metabolic regulator AMPK plays a pivotal role.
Overexpression of AMP-activated kinase sensitizes Sorafenib-resistant Hepatocellular cancer cells and decreases stem cell markers
Contributors: Ines Diaz-Laviada
... Data used to generate figures of the paper by Bort A, Sánchez BG, Mateos-Gómez PA, Vara-Ciruelos D, Rodríguez-Henche N, Díaz-Laviada I. “Targeting AMP-activated kinase impacts hepatocellular cancer stem cells induced by long-term treatment with sorafenib” Mol Oncol. 2019 May;13(5):1311-1331. doi: 10.1002/1878-0261.12488. In this study we demonstrate that the AMP-activated kinase AMPK reverse the Cancer Stem cell features of Hepatocellular CSC.
Data for: Nuclear genome shuffling increases recombinant protein expression in the chloroplast of Chlamydomonas reinhardtii
Contributors: Francis Fields, Stephen Mayfield, Miller Tran, Joseph T. Ostrand
... Supplemental data for "Nuclear genome shuffling significantly increases production of chloroplast-based recombinant protein in Chlamydomonas reinhardtii" by Fields et al.
Contributors: Luca Iandoli
... The data have been used to test the following research hypotheses H1: Perceived Elegance is inversely correlated to expected effort; H2: Perceived Elegance is positively correlated with the EC of a product interface. H3: Elegance is positively correlated to the actual effort of using a new product. Perceived was elegance was measured through an online survey (see survey) in which participants were asked to rank several models of an appliance control panel in order of perceived elegance using a one to 5 scale (1 most elegant, 5 least elegant) Expected effort was measured through an online survey (see survey) in which participants were asked to rank several models of an appliance control panel in order of perceived elegance using a one to 5 scale (the easiest to use) to 5 ( the hardest to use) Effective complexity is a metric described in the file Microwave EC.ppt. The EC value is computed based on the spatial organization of control elements on the panel (knobs, buttons, screens, etc.). Actual effort was measured through total execution time of the three tasks by every participant, including possible mistakes and subsequent new attempts. The Kruskal Wallis test and post hoc test are reported in the data actual effort folder
Regional heterogeneity in gene expression, regulation and coherence in hippocampus and prefrontal cortex across development and in schizophrenia. Collado-Torres et al, Neuron, 2019.
Contributors: Leonardo Collado-Torres, Emily E Burke, Amy Peterson, Joo Heon Shin, Richard E Straub, Nina Rajpurohit, Stephen A Semick, William S Ulrich, BrainSeq Consortium, Amanda J Price
... This dataset contains the Supplementary Figures and Tables for the "Regional heterogeneity in gene expression, regulation and coherence in hippocampus and prefrontal cortex across development and in schizophrenia" article by Collado-Torres et al, Neuron, 2019. Please cite the original article as well as the data itself if appropriate. For clarifications about the figures or data, please contact us by creating an issue at https://github.com/LieberInstitute/brainseq_phase2/issues. Thank you!