Astrocyte-derived extracellular vesicles enhance the electrophysiological function and cell survival of human cortical neurons in vitro
Contributors: Deok-Ho Kim, Changho Chun, Alec Smith
... The data uploaded here are for the manuscript titled 'Astrocyte-derived extracellular vesicles enhance the electrophysiological function and cell survival of human cortical neurons in vitro '
Integrated approach for data acquisition, visualization and processing of analog polarographic systems for bioenergetics studies
Contributors: Lucas Potter, Dean Krusienski , Jesse Kennedy, Charles Hoppel, Nicola Lai
... Graphical user interface for bioenergetics data. This is the supplemental material of the manuscript "Integrated approach for data acquisition, visualization and processing of analog polarographic systems for bioenergetics studies" that includes the graphical user interface (GUI), user guide and file examples generated with the GUI and a video tutorial. The GUI allows to calibrate oxygen sensor, continuous visualization, decimation and analysis of data recorded with a comma separated values format. GUI can be used to visualize and analyze data of analog polarographic systems. The GUI allows to overcome cumbersome calculations to extract bioenergetic data from oxygen kinetics, enhance the accuracy of RCR and ADP/O ratio, and optimize the decimation of kinetic data for computational applications.
Contributors: Eusebio de la Fuente
... This dataset contain the code, images and video results of the analysed algorithms: LBP|VAR, CLBP and the convolutional neural network ResNet50.
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Contributors: Francois Lambert
... Title: Swim style-dependent utricular activation ensures gaze-stabilization during early development
Contributors: Mei-hui Liu
... This set of supplementary data is related to the article “Blended Cross-tier Teacher Development: Designing Online Video-based Pragmatic Assessment.” It contains 1) survey data, 2) data collection methods, and 3) samples of edited video clips retrieved from Youtube links and deigned discourse completion tests. The participants’ interview content and reflection entries cannot be exposed to the public under the ethics guidelines of National Cheng Kung University Human Research Ethics Committee in Taiwan.
Data/Software for "Presynaptic Mitochondria Volume and Abundance Increase During Development of a High-Fidelity Synapse"
Contributors: Connon I. Thomas, Christian Keine, Satoko Okayama, Rachel Satterfield, Morgan Musgrove, Debbie Guerrero-Given, Naomi Kamasawa, Samuel M. Young, Jr.
... Contains data and software from the publication: "Presynaptic Mitochondria Volume and Abundance Increase During Development of a High-Fidelity Synapse" published in the The Journal of Neuroscience (https://doi.org/10.1523/JNEUROSCI.0363-19.2019). The preprint to this data set has been published on bioRxiv (https://doi.org/10.1101/689653). In this study, we created a helper-dependent adenoviral vector (HdAd) to co-express cytoplasmic EGFP and a genetically encoded peroxidase marker (mito-APEX2) at the calyx of Held, an excellent model for deciphering regulatory mechanisms of presynaptic function. ABSTRACT: The calyx of Held, a large glutamatergic presynaptic terminal in the auditory brainstem undergoes developmental changes to support the high action-potential firing rates required for auditory information encoding. In addition, calyx terminals are morphologically diverse which impacts vesicle release properties and synaptic plasticity. Mitochondria influence synaptic plasticity through calcium buffering and are crucial for providing the energy required for synaptic transmission. Therefore, it has been postulated that mitochondrial levels increase during development and contribute to the morphological-functional diversity in the mature calyx. However, the developmental profile of mitochondrial volumes and subsynaptic distribution at the calyx of Held remains unclear. To provide insight on this, we developed a helper-dependent adenoviral vector (HdAd) that expresses the genetically encoded peroxidase marker for mitochondria, mito-APEX2, at the mouse calyx of Held. We developed protocols to detect labeled mitochondria for use with serial block face scanning electron microscopy to carry out semi-automated segmentation of mitochondria, high-throughput whole terminal reconstruction and presynaptic ultrastructure in mice of either sex. Subsequently, we measured mitochondrial volumes and subsynaptic distributions at the immature postnatal day 7 (P7) and the mature (P21) calyx. We found an increase of mitochondria volumes in terminals and axons from P7 to P21 but did not observe differences between stalk and swelling subcompartments in the mature calyx. Based on these findings, we propose that mitochondrial volumes and synaptic localization developmentally increase to support high firing rates required in the initial stages of auditory information processing. Data are sorted by the figures they appear in. Media (movies and 3D models) and custom-written software are located in separate folders.
Is There Variability in Scoring of Student Surgical OSCE Performance Based on Examiner Experience and Expertise?
Contributors: Claire L. Donohoe
... Scripted videos of simulated student performance in an OSCE at two standards (clear pass and borderline) were prospectively awarded a global score based on two different rating scales by a range of clinical assessors. Results were analysed by examiner experience and content expertise.
Improve the interfacial adhesion, corrosion resistance and combustion properties of aluminum powder by modification of nickel and dopamine
Contributors: Li Liang, Xiaode Guo, Xin Liao, Zhipeng Chang
... To improve the interface incompatibility, poor corrosion resistance, and limited combustion efficiency of aluminum powder for propellant applications. The surface of the naked aluminum was coated with a layer of dense nickel of about 100 nm by replacement method. A polydopamine film was then deposited by self-polymerization. The double-layer structure and chemical composition were investigated. The polydopamine content of composite particles was 2.3% by thermal analysis. And its heat release around 1050 C reached 4137 J g-1, which was much higher than that of aluminum of 1644 J g-1. The surface energies was used to calculate the theoretical adhesion work of aluminum and composite particles to propellant binder. The adhesion work after modification was increased from 62.33 mN m-1 to 74.89 mN m-1. No matter in the weak acid/alkaline or neutral environment, the composite particles exhibited excellent stability at 50 C. In addition, the combustion test revealed that the composite particles had higher combustion efficiency, smaller condensed phase product size, and stronger ignition ability. All the conclusions indicate after modification by nickel and dopamine, the comprehensive performance of aluminum is improved, and it may be used as an energy component in propellants or other energetic fields.
DATA: Accommodation of manganese into hydroxyapatite and segregation pathway involved in the formation of Mn3O4 nanoparticles
Contributors: Euler Araujo dos Santos
... Raw data and Rietveld refined data used in the article.
Contributors: UnaElsLive Natra, mdgmatest5 live
... RDM - File Type Support 21May2019 ElsCustomer Apart from .u3d all files preview [ .obj / .ply / .vtk / .stl / .ent / .brk / .pdb / .pse / .mol / .mol2 / .cif / .u3d / .dcm / .nii] - .pse is not supported