Data/Software for "Presynaptic Mitochondria Volume and Abundance Increase During Development of a High-Fidelity Synapse"
Contributors: Connon I. Thomas, Christian Keine, Satoko Okayama, Rachel Satterfield, Morgan Musgrove, Debbie Guerrero-Given, Naomi Kamasawa, Samuel M. Young, Jr.
... Contains data and software from the publication: "Presynaptic Mitochondria Volume and Abundance Increase During Development of a High-Fidelity Synapse" published in the The Journal of Neuroscience (https://doi.org/10.1523/JNEUROSCI.0363-19.2019). The preprint to this data set has been published on bioRxiv (https://doi.org/10.1101/689653). In this study, we created a helper-dependent adenoviral vector (HdAd) to co-express cytoplasmic EGFP and a genetically encoded peroxidase marker (mito-APEX2) at the calyx of Held, an excellent model for deciphering regulatory mechanisms of presynaptic function. ABSTRACT: The calyx of Held, a large glutamatergic presynaptic terminal in the auditory brainstem undergoes developmental changes to support the high action-potential firing rates required for auditory information encoding. In addition, calyx terminals are morphologically diverse which impacts vesicle release properties and synaptic plasticity. Mitochondria influence synaptic plasticity through calcium buffering and are crucial for providing the energy required for synaptic transmission. Therefore, it has been postulated that mitochondrial levels increase during development and contribute to the morphological-functional diversity in the mature calyx. However, the developmental profile of mitochondrial volumes and subsynaptic distribution at the calyx of Held remains unclear. To provide insight on this, we developed a helper-dependent adenoviral vector (HdAd) that expresses the genetically encoded peroxidase marker for mitochondria, mito-APEX2, at the mouse calyx of Held. We developed protocols to detect labeled mitochondria for use with serial block face scanning electron microscopy to carry out semi-automated segmentation of mitochondria, high-throughput whole terminal reconstruction and presynaptic ultrastructure in mice of either sex. Subsequently, we measured mitochondrial volumes and subsynaptic distributions at the immature postnatal day 7 (P7) and the mature (P21) calyx. We found an increase of mitochondria volumes in terminals and axons from P7 to P21 but did not observe differences between stalk and swelling subcompartments in the mature calyx. Based on these findings, we propose that mitochondrial volumes and synaptic localization developmentally increase to support high firing rates required in the initial stages of auditory information processing. Data are sorted by the figures they appear in. Media (movies and 3D models) and custom-written software are located in separate folders.
Contributors: Peter Toson, Johannes G. Khinast
... Update Version 2: Link to Data in Brief article: https://doi.org/10.1016/j.dib.2019.104672 Pre-processed particle data obtained from DEM simulations. For each particle, the initial position in the twin-screw feeder, the particle radius, and the residence time is given. The data is available for 3 fill levels in the feeder (100%, 66%, 40%). Included in the dataset are example RTDs obtained from the particle data, a minimum working example for a Python script that calculates the RTD of particles in two spatial regions of the feeder, and a video of the complete discharge process rendered from the DEM results.
Contributors: UnaElsLive Natra
... MD-2831 Update Java version in the Docker containers
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Formation of artifact-hydride in Zr-containing amorphous alloys during sample preparation and atom probe tomography
Contributors: chetarpa yipyintum
... Hydride formation in Zr-containing amorphous alloys as an experimental artifact was investigated utilizing atom probe tomography, transmission electron microscopy, and focused ion beam with normal and cryogenic conditions. The amount of hydrogen existing in the atom probe specimens decreased significantly by utilizing focused ion beam milling under cryogenic condition. Also, the formation of hydride was confirmed by diffraction pattern analysis in the remaining tip of the specimen after the atom probe tomography experiment. With those collected pieces of evidence, sources of hydrogen in the atom probe tomography were discussed.
DATA: Accommodation of manganese into hydroxyapatite and segregation pathway involved in the formation of Mn3O4 nanoparticles
Contributors: Euler Araujo dos Santos
... Raw data and Rietveld refined data used in the article.
Contributors: Eusebio de la Fuente
... This dataset contain the code, test videos and the results of the developed algorithm. The detection is based on texture analysis using the LBP technique.
Contributors: jasvinder pal Singh, Madhav Institute of Technology & Science, Sakshi Arora, UDAY PRATAP SINGH
... Previous research in gait recognition and available dataset focused on single subjects. But in real time environment (such as airport, metro stations etc.) more than one person walk in a group and occlusion factors affects the performance of gait recognition. Therefore, we presented a new dataset which focused dynamic occlusion scenario. We constructed two categories of gait dataset i.e. first, subjects walk in a group (SMVDU-Multi-Gait) and second, subjects walk separately (SMVDU-Single-Gait). The purpose of this dataset to analyze the variations in gait patterns when same subject walk in a group or individually and also to recognize the target subject in Multi-Gait.
Contributors: UnaElsLive Natra, mdgmatest5 live
... RDM - File Type Support 21May2019 ElsCustomer Apart from .u3d all files preview [ .obj / .ply / .vtk / .stl / .ent / .brk / .pdb / .pse / .mol / .mol2 / .cif / .u3d / .dcm / .nii] - .pse is not supported
Contributors: Guillem Camprodon, Viktor Smari, Dorian Somers
... The Smart Citizen Web is the main front-end website for the Smart Citizen Platform. It provides a visual website where the project environmental sensors can be accessed in near real time to facilitate the exploration of data with other contextual data (maps, keywords) and processed reports. This is especially important towards citizens engaging at each local site having a sense of ownership over a technology intervention has been associated with sustained community engagement. It connects with the Smart Citizen Platform using its REST API. This work is led by IAAC Fab Lab Barcelona team under the framework of iSCAPE (Improving Smart Control of Air Pollution in Europe) project, which is funded by the European Community’s H2020 Programme (H2020-SC5-04-2015) under the Grant Agreement No. 689954. All the authors acknowledge the funding received via the iSCAPE project.
Supplementary material for the PhD thesis of Rickman, J., "Modelling microtubule dynamics and microtubule-motor self-organisation", UCL, 2019.
Contributors: Jamie Rickman
... Simulation configuration files, documentation and supplementary movies.