Contributors: Jianming Zhang
... Video S1 shows the process that the liquid level in the cylinder gradually decreased between 0 and 12 h, while the thickness of the bright thin layer at the bottom increased, which is the supplementary datum of Figure 1a. Video S2 presents that the gel, supported on the filter paper, can be obtained by decanting the upper suspension.
Contributors: Ulrich Stefan Bauer, Rosanne Fransisca van de Wijdeven , Ioanna Sandvig, Axel Sandvig
... Abolition of contractile activity in in vitro neuromuscular junctions within multi-nodal microfluidic chips using alpha-Bungarotoxin. Chips seeded as described above were allowed to mature for 21 days after seeding. Chips were selected for high contractile activity of myotubes that had visual contact with axons (more than 1 contraction per minute over 5 minutes with at least 1 contraction in each minute, the day prior to the experiment). Video of pre-experiment activity was recorded for 1 min followed by counting of the total number of contractions in 10 min. The cells were allowed to recover for 1 h at 37°C, 5% CO2 before addition of 1:100 of α-BTX at a final concentration of 1.25μM or of 0.75μl sterile PBS and incubation of 10 min at 37°C, 5% CO2. The α-BTX was only added to the central well containing the myotubes, which was fluidically isolated by hydrostatic pressure throughout the incubation. Another 1 min video of activity after intervention was recorded before counting the total number of contractions in 10 min. Cells were fixed immediately after the final count. For quantitative analysis the experiment was repeated with 10 min video being recorded as baseline followed by 1 h recovery at 37°C, 5% CO2 and treatment. Another 10 min video was recorded after treatment, and blinded quantification was carried out for both.
Data for: Synthesis and evaluation of 89Zr-labeled and long-lived GLP-1 receptor agonists for PET imaging
Contributors: Christian Borch Jacobsen, Franck Denat, James McGuire, Magnus Gustafsson, Ibai Valverde, Kirsten Raun, Pierre Adumeau, René Raavé, Mathieu Moreau, Sandra Heskamp
... Appendix B. PET imaging 3D rendered images. 1. PET 3D of 4-89Zr 2. PET 3D of 5-89Zr
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Data for: Potential field continuation in spatial domain: A new kernel function and its numerical scheme
Contributors: Chunhui Tao, zhikui guo
... (1) conti2d-win10.zip includes manual, source code and pre-compiled executable file with and without GUI for Win10 system. (2) conti2d-master.zip includes manual, source code for Linux and Mac OS. (3) Tutorial_conti2d.mp4 is a video tutorial for executable program of conti2d with GUI.
Contributors: Alexey Sukhanov
... The concomitant change of the pH as acquired by the pH meter and the related color change of the sensor membrane
Astrocyte-derived extracellular vesicles enhance the electrophysiological function and cell survival of human cortical neurons in vitro
Contributors: Deok-Ho Kim, Changho Chun, Alec Smith
... The data uploaded here are for the manuscript titled 'Astrocyte-derived extracellular vesicles enhance the electrophysiological function and cell survival of human cortical neurons in vitro '
Contributors: Annunziata Pirro, Hemantha Wijesekera, Tommy Jensen, Luca Centurioni, S Jinadasa, Harindra Joseph Fernando
... Sea Surface Height Animation for 2015
Contributors: Yue Qu
... Video 1: Active detachment of bacterial cells from the frontline of interstitial biofilms and free movement of planktonic cells or small bacterial clusters were noticed after 24 h of biofilm culture in a humid chamber. Video 2: The dispersed cells seeded a nearby area and re-established a standalone “daughter” biofilm. The “daughter” biofilms expanded to merge with the primary biofilm and lead to migration of the interstitial biofilm.
Contributors: zuheir khlaif
... Interviews with teachers about their experience with STEm professional development in Palestine.
Contributors: Neil Zhang
... Drosophila grooming ethograms, MATLAB code for grooming model simulation and one example video of fly-on-a-ball experiment Ethograms can be opened in MATLAB. Different numbers stand for different behaviors: For dusting experiments: 1.Front Leg rubbing 2. Head cleaning 3. Abdominal cleaning 4. Back Leg rubbing 5. Wing cleaning 6. Walking 7. Standing For optogenetic experiments in free-moving flies: 1.Front Leg rubbing 2. Head cleaning 3. Abdominal cleaning 4. Back Leg rubbing 5. Wing cleaning 6. Thoracic Cleaning 7. Walking 8. Standing For fly-on-a-ball experiments: 1. Anterior grooming 2. Posterior grooming 3. Walking 4. Standing