Contributors: Francois Lambert
... Title: Swim style-dependent utricular activation ensures gaze-stabilization during early development
Contributors: Mei-hui Liu
... This set of supplementary data is related to the article “Blended Cross-tier Teacher Development: Designing Online Video-based Pragmatic Assessment.” It contains 1) survey data, 2) data collection methods, and 3) samples of edited video clips retrieved from Youtube links and deigned discourse completion tests. The participants’ interview content and reflection entries cannot be exposed to the public under the ethics guidelines of National Cheng Kung University Human Research Ethics Committee in Taiwan.
Data/Software for "Presynaptic Mitochondria Volume and Abundance Increase During Development of a High-Fidelity Synapse"
Contributors: Connon I. Thomas, Christian Keine, Satoko Okayama, Rachel Satterfield, Morgan Musgrove, Debbie Guerrero-Given, Naomi Kamasawa, Samuel M. Young, Jr.
... Contains data and software from the publication: "Presynaptic Mitochondria Volume and Abundance Increase During Development of a High-Fidelity Synapse" published in the The Journal of Neuroscience (https://doi.org/10.1523/JNEUROSCI.0363-19.2019). The preprint to this data set has been published on bioRxiv (https://doi.org/10.1101/689653). In this study, we created a helper-dependent adenoviral vector (HdAd) to co-express cytoplasmic EGFP and a genetically encoded peroxidase marker (mito-APEX2) at the calyx of Held, an excellent model for deciphering regulatory mechanisms of presynaptic function. ABSTRACT: The calyx of Held, a large glutamatergic presynaptic terminal in the auditory brainstem undergoes developmental changes to support the high action-potential firing rates required for auditory information encoding. In addition, calyx terminals are morphologically diverse which impacts vesicle release properties and synaptic plasticity. Mitochondria influence synaptic plasticity through calcium buffering and are crucial for providing the energy required for synaptic transmission. Therefore, it has been postulated that mitochondrial levels increase during development and contribute to the morphological-functional diversity in the mature calyx. However, the developmental profile of mitochondrial volumes and subsynaptic distribution at the calyx of Held remains unclear. To provide insight on this, we developed a helper-dependent adenoviral vector (HdAd) that expresses the genetically encoded peroxidase marker for mitochondria, mito-APEX2, at the mouse calyx of Held. We developed protocols to detect labeled mitochondria for use with serial block face scanning electron microscopy to carry out semi-automated segmentation of mitochondria, high-throughput whole terminal reconstruction and presynaptic ultrastructure in mice of either sex. Subsequently, we measured mitochondrial volumes and subsynaptic distributions at the immature postnatal day 7 (P7) and the mature (P21) calyx. We found an increase of mitochondria volumes in terminals and axons from P7 to P21 but did not observe differences between stalk and swelling subcompartments in the mature calyx. Based on these findings, we propose that mitochondrial volumes and synaptic localization developmentally increase to support high firing rates required in the initial stages of auditory information processing. Data are sorted by the figures they appear in. Media (movies and 3D models) and custom-written software are located in separate folders.
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Is There Variability in Scoring of Student Surgical OSCE Performance Based on Examiner Experience and Expertise?
Contributors: Claire L. Donohoe
... Scripted videos of simulated student performance in an OSCE at two standards (clear pass and borderline) were prospectively awarded a global score based on two different rating scales by a range of clinical assessors. Results were analysed by examiner experience and content expertise.
Improve the interfacial adhesion, corrosion resistance and combustion properties of aluminum powder by modification of nickel and dopamine
Contributors: Li Liang, Xiaode Guo, Xin Liao, Zhipeng Chang
... To improve the interface incompatibility, poor corrosion resistance, and limited combustion efficiency of aluminum powder for propellant applications. The surface of the naked aluminum was coated with a layer of dense nickel of about 100 nm by replacement method. A polydopamine film was then deposited by self-polymerization. The double-layer structure and chemical composition were investigated. The polydopamine content of composite particles was 2.3% by thermal analysis. And its heat release around 1050 C reached 4137 J g-1, which was much higher than that of aluminum of 1644 J g-1. The surface energies was used to calculate the theoretical adhesion work of aluminum and composite particles to propellant binder. The adhesion work after modification was increased from 62.33 mN m-1 to 74.89 mN m-1. No matter in the weak acid/alkaline or neutral environment, the composite particles exhibited excellent stability at 50 C. In addition, the combustion test revealed that the composite particles had higher combustion efficiency, smaller condensed phase product size, and stronger ignition ability. All the conclusions indicate after modification by nickel and dopamine, the comprehensive performance of aluminum is improved, and it may be used as an energy component in propellants or other energetic fields.
mHeart - mHealthCare Platform Adapted to the Heart Transplant Population - Technical Specifications and Software Source Code
Contributors: Mar Gomis-Pastor, Vicente Pellicer, Miriam Ors, Mª Antonia Mangues
... The information is provided to increase the data transparency and the scalability of eHealth research and particularly the mHeart studies scalability. This dataset contains relevant information on: - The mHealthCare Platform Technological Description and Specifications. This file aims to describe the general mHealthcare Platform developed by the private firm which has been thereafter adapted to the heart transplant specifications (mHeart System). - The mHeart source code description of the 3 applications (website, app Android, app Apple) and storage location. - A video of the clinical use of the mHeart mobile application. - An apk file with a DEMO (Spain Version 2015). Please, notice that this is a dynamic process. Multiples versions will be uploaded since mHeart was developed. Consult the DEMO access directly on Stores. - User Guidelines (Spain Version 2015) - Scientific Authorship Statement - mHeart trademark - the mHeart System funding and reimbursement model. The mHealthCare Platform is an internet-based system to carry out integral healthcare for disease and therapy management in any population. The first version uploaded in Hospital de la Santa Creu i Sant Pau (HSCSP) was directed at transplant populations and was specifically adapted to the heart transplant population in the ambulatory setting. The mHeart program has been classified by authors as a Behavior Intervention Technology (BIT) to facilitate the following overall clinical goals: (1) health behavior change (i.e. increase patients’ healthy behaviors and prevent the onset of disease); and (2) targeted disease management (i.e. facilitate therapeutic interventions and improve patient’s self-management). The mHeart tool is a mobile phone app connected to a website for use by providers and patients. The tool can be used simultaneously on distinct devices to facilitate caregiver and guardian support. The mobile application can be downloaded for free from the Google and Apple online stores. The website link is salud.trilema.es. The system was developed by Socioemprende SL Technical Team. Scientific advice was provided by the heart transplant interdisciplinary healthcare team and directed by the Pharmacy Department, HSCSP. For further questions about the System, please contact with the scientific coordinator Mar Gomis-Pastor: firstname.lastname@example.org
Contributors: UnaElsLive Natra
... MD-2831 Update Java version in the Docker containers
Morphology and lifestyle of endophytic fungi associated with roots of Gaylussacia brasiliensis (Ericaceae) in southern Brazil sand dunes
Contributors: Kelly Besen
... Gaylussacia brasiliensis hair roots, revealing wide morphological diversity. Pezizomicotina fungi have been found in healthy ericaceous roots, showing several morphological structures of the symbiotic association. Morphological and sequencing analyses of seven isolates revealed that they belong to the genera Penicillium, Paraconiothyrium, Cladophialofora, Knufia, Diaporthe, Chaetomium, and Arcopilus. It appears that there are as many association structures as there are fungi capable of producing them.
Asymmetric centromeres differentially coordinate with mitotic machinery to ensure biased sister chromatid segregation in germline stem cells
Contributors: Rajesh Ranjan
... Many stem cells utilize asymmetric cell division (ACD) to produce a self-renewed stem cell and a differentiating daughter cell. How non-genic information could be inherited differentially to establish distinct cell fates is not well understood. Here, we report a series of spatiotemporally regulated asymmetric components, which ensure biased sister chromatid attachment and segregation during ACD of Drosophila male germline stem cells (GSCs). First, sister centromeres are differentially enriched with proteins involved in centromere specification and kinetochore function. Second, temporally asymmetric microtubule activities and polarized nuclear envelope breakdown allow for the preferential recognition and attachment of microtubules to asymmetric sister kinetochores and sister centromeres. Abolishment of either the asymmetric sister centromeres or the asymmetric microtubule activities results in randomized sister chromatid segregation. Together, these results provide the cellular basis for partitioning epigenetically distinct sister chromatids during stem cell ACDs, which opens new directions to study these mechanisms in other biological contexts.
DATA: Accommodation of manganese into hydroxyapatite and segregation pathway involved in the formation of Mn3O4 nanoparticles
Contributors: Euler Araujo dos Santos
... Raw data and Rietveld refined data used in the article.