Contributors: UnaElsLive Natra, mdgmatest5 live
... RDM - File Type Support 21May2019 ElsCustomer Apart from .u3d all files preview [ .obj / .ply / .vtk / .stl / .ent / .brk / .pdb / .pse / .mol / .mol2 / .cif / .u3d / .dcm / .nii] - .pse is not supported
Contributors: Aaley, Uday Raj, Bodt, Timotheus Adrianus
... This data set contains 8 video files made of the last two known Kusunda speakers from Nepal, Gyani Maiya Sen Kusunda and Kamala Khatri (Sen Kusunda), in February and April 2019, in Kulmore, Dang Deokhuri, Nepal. The recordings constitute discussion between the participants, showing how to use flint, how to make a lean-to, and how to dig for tubers. This material is made freely available to everyone for informative or scientific purposes as long as the source (this DOI) / the collectors are properly credited. Please note that use of the material for commercial purposes of any kind, which includes conversion into commercial audio-visual media (documentaries etc.), (paid) public screening other than for educational purposes, storage and dissemination through sites that require registration & payment for access, or sites that rely on advertisement (including YouTube) is not permitted without specific written consent from the speakers and their community, obtained through the collectors of the material. By downloading our material, you agree to these restrictions. This data set falls under the Create Commons Attribution 4.0 International / Attribution-NonCommercial-ShareAlike (CC BY-NC-SA) license. This license lets you remix, tweak, and build upon this work non-commercially, as long as you credit us and license your new creations under the identical terms. License Deed on https://creativecommons.org/licenses/by-nc-sa/4.0/. Legal Code on https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode. We greatly value feedback, suggestions, advice, analysis etc. based on this material which will help in the description of the Kusunda language, especially any comments and suggestions that will enable the revitalisation of the language, including a standardisation of the phonology and a phonologically consistent but also practical orthography in both देवनागरी Devanāgarī and Roman script. Uday Raj Aaley: aarambhkhabar (at) gmail (dot) com Tim Bodt: bodttim (at) gmail (dot) com
A nemertean excitatory peptide/CCHamide regulates ciliary swimming in the larvae of Lineus longissimus
Contributors: Thiel, Daniel, Bauknecht, Philipp, Jékely, Gáspár, Hejnol, Andreas
... Background: The trochozoan excitatory peptide (EP) and its ortholog, the arthropod CCHamide, are neuropeptides that are only investigated in very few animal species. Previous studies on different trochozoan species focused on their physiological effect in adult specimens, demonstrating a myo-excitatory effect, often on tissues of the digestive system. The function of EP in the planktonic larvae of trochozoans has not yet been studied.Results: We surveyed transcriptomes from species of various spiralian (Orthonectida, Nemertea, Brachiopoda, Entoprocta, Rotifera) and ecdysozoan taxa (Tardigrada, Onychophora, Priapulida, Loricifera, Nematomorpha) to investigate the evolution of EPs/CCHamides in protostomes. We found that the EPs of several pilidiophoran nemerteans show a characteristic difference in their C-terminus. Deorphanization of a pilidiophoran EP receptor showed, that the two splice variants of the nemertean Lineus longissimus EP activate a single receptor. We investigated the expression of EP in L. longissimus larvae and juveniles with customized antibodies and found that EP positive nerves in larvae project from the apical organ to the ciliary band and that EP is expressed more broadly in juveniles in the neuropil and the prominent longitudinal nerve cords. While exposing juvenile L. longissimus specimens to synthetic excitatory peptides did not show any obvious effect, exposure of larvae to either of the two EPs increased the beat frequency of their locomotory cilia and shifted their vertical swimming distribution in a water column upwards.Conclusion: Our results show that EP/CCHamide peptides are broadly conserved in protostomes. We show that the EP increases the ciliary beat frequency of L. longissimus larvae, which shifts their vertical distribution in a water column upwards. Endogenous EP may be released at the ciliary band from the projections of apical organ EP positive neurons to regulate ciliary beating. This locomotory function of EP in L. longissimus larvae stands in contrast to the repeated association of EP/CCHamides with its myo-excitatory effect in adult trochozoans and the general association with the digestive system in many protostomes.
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Contributors: Bodt, Timotheus Adrianus
... This collection of videos and photos displays the three days of Tawang Dungjur festival in 2013. It was held for three days, on 1st, 2nd and 3rd October 2013. The files are saved on three separate DOIs, each for one of the dates. The focus of these recordings is mainly on the dances are that unique to the Tawang Dungjur festival, rather than those in common with the tshecu festivals organised in Tibet, Bhutan and, indeed, across much of the Tibetan Buddhist world. These ‘local’ dances not only teach the audience lessons from Buddhism, but are also greatly entertaining. A detailed description of these dances would be welcomed. Unfortunately, by the time these files were archived and saved on Zenodo, five years had passed and not much about the order and meaning of the dances could be remembered. In due course of time, a second version of this accompanying document will contain more detailed information on what is exactly depicted in the videos and pictures.
Contributors: Eccles, David Andrew
... A spiral representation of the longest sequence found by Alex Payne et al., which at the time of upload was the longest observed public single-pore nanopore sequence. This video shows about 30kb of the sequence at once, and zooms through the sequence at about 20kb per second in the animated GIF, or about 48kb per second in the AVI file.
Contributors: Rourk, William
... Fralin 3D ; Laser scan data of a Greek pyxis from the UVA Fralin Museum collection.
Molecular modeling of the human P2Y14 receptor: A template for structure-based design of selective agonist ligands
Contributors: Kevin Trujillo, Silvia Paoletta, Evgeny Kiselev, Kenneth A. Jacobson
... The P2Y14 receptor (P2Y14R) is a Gi protein-coupled receptor that is activated by uracil nucleotides UDP and UDP-glucose. The P2Y14R structure has yet to be solved through X-ray crystallography, but the recent agonist-bound crystal structure of the P2Y12R provides a potentially suitable template for its homology modeling for rational structure-based design of selective and high-affinity ligands. In this study, we applied ligand docking and molecular dynamics refinement to a P2Y14R homology model to qualitatively explain structure–activity relationships of previously published synthetic nucleotide analogues and to probe the quality of P2Y14R homology modeling as a template for structure-based design. The P2Y14R model supports the hypothesis of a conserved binding mode of nucleotides in the three P2Y12-like receptors involving functionally conserved residues. We predict phosphate group interactions with R2536.55, K2777.35, Y2566.58 and Q2606.62, nucleobase (anti-conformation) π–π stacking with Y1023.33 and the role of F1915.42 as a means for selectivity among P2Y12-like receptors. The glucose moiety of UDP-glucose docked in a secondary subpocket at the P2Y14R homology model. Thus, P2Y14R homology modeling may allow detailed prediction of interactions to facilitate the design of high affinity, selective agonists as pharmacological tools to study the P2Y14R.