Contributors: Rikke Meyer
... Inorganic fouling is one of the challenging problems in heat exchanger applications. One approach to mitigate fouling is to employ surface coatings. In this study, we evaluated the feasibility of surface initiated polymerization (SIP) as thin coating technology to mitigate CaCO3 formation for heat transfer applications. The extent of formation of CaCO3 on different types of poly(oligoethyleneglycol) methacrylate brushes (POEGMA) was investigated under stagnant and flow heat-exchanging conditions. Polymer brushes of high graft density reduced the surface coverage of CaCO3 more effectively than the low graft density brushes. By contrast, the thickness of the brush did not correlate with the surface coverage of CaCO3. The comparison of stagnant and flow experiments revealed that the antiscaling effect of POEGMA brushes originate due to reduced adhesion of CaCO3. Finally, the SIP process was successfully scaled-up to coat commercial heat exchanger plates with thickness and homogeneity comparable to lab-scale surfaces. Under industrial testing, the POEGMA brushes extended the performance by 50 h before the commencement of complete blockage.
Contributors: Gabriel Fiorin, Andrea Sanchez-Vallet , Daniela Thomazella, Paula Prado, Leandro Nascimento, Antonio Figueira, Bart Thomma, Goncalo Pereira, Paulo Jose Teixeira
... Source data of the paper "Suppression of plant immunity by fungal chitinase-like effectors".
Contributors: Saroj Chakraborty
... Salt-responsive Metabolite, beta-hydroxybutyrate, Attenuates Hypertension Chakraborty, S1, Galla, S1, Cheng X1, Yeo, J1, Mell, B1, Singh V1, Yeoh BS1, Saha P1, Mathew, AV2, Vijay-Kumar, M1, Joe B*1. 1Program in Physiological Genomics, Microbiome Consortium, Center for Hypertension and Personalized Medicine, Department of Physiology and Pharmacology, University of Toledo College of Medicine and Life Sciences, Toledo, OH, USA. 2 Department of Internal Medicine-Nephrology, University of Michigan, Ann Arbor, MI, USA. *Corresponding Author: Bina Joe, firstname.lastname@example.org Summary Dietary salt reduction and exercise are lifestyle modifications prescribed for salt-sensitive hypertensives. While exercise is known to have prominent metabolic effects, such effects of lower dietary salt are just emerging. Studies indicate that salt has adverse effect on metabolic syndrome, of which, hypertension is a hallmark. We hypothesized that dietary salt impacts metabolism in a salt-sensitive model of hypertension. Untargeted metabolomic profiling of rats with differential salt (NaCl) diets revealed that a high salt-induced increase in blood pressure was associated with lower circulating levels of the ketone body, beta-hydroxybutyrate (βOHB). Specific rescue of the βOHB levels by nutritional supplementation of its precursor, 1,3-butanediol attenuated hypertension and protected kidney disease despite the high salt intake. This beneficial effect of βOHB was observed independent of the gut-microbiotal effects of salt or the Th17-mediated renal effects. Instead, the mechanism for the observed renoprotective effect of βOHB on salt-sensitive hypertension was the βOHB-mediated inhibition of the pro-inflammatory cascade by the Nlrp3 inflammasome. In summary, the juxtaposed effects of dietary salt and exercise on salt-sensitive hypertension, which decrease and increase βOHB respectively, indicates that nutritional supplementation of a precursor of βOHB could be considered to provide a similar benefit to salt-sensitive hypertension as exercise.
Contributors: Agnieszka Tudek
... A nuclear export block triggers the decay of newly synthesized polyadenylated RNA Agnieszka Tudek1, Manfred Schmid1, Marius Makaras1, J. David Barrass2, Jean D. Beggs2, Torben Heick Jensen1* 1Department of Molecular Biology and Genetics, Aarhus University, C. F. Møllers Allé 3, building 1130, 8000 Aarhus C, Denmark, 2Wellcome Centre for Cell Biology, University of Edinburgh, Edinburgh EH9 3BF, UK *corresponding author and lead contact: email@example.com Keywords: nuclear export of pA+ RNA, nuclear degradation of pA+ RNA, Mex67p, Nab2p, transcription. ABSTRACT Genomes are promiscuously transcribed necessitating mechanisms that facilitate the sorting of RNA for function or destruction. The polyA (pA) tail is one such distinguishing feature, which in the Saccharomyces cerevisiae nucleus is bound by the Nab2p protein, yielding transcript protection. As Nab2p also contacts the main nuclear export factor Mex67p, we asked whether transport kinetics contributes to RNA sorting. Indeed, 3’end sequencing of newly transcribed pA+ RNAs demonstrates that nuclear depletion of Mex67p elicits their instant and global decay. A similar phenotype is evident upon inactivation of other export factors and proportional to the amount of nuclear pA+ RNA. As RNA expression is partially rescued by Nab2p over-expression, we propose that an export-block out-titrates Nab2p onto nuclear retained pA+ RNA, reducing the pool of Nab2p available to protect new transcripts. More generally, we suggest that nuclear RNA decay, negotiated by Nab2p availability, aids in balancing cellular transcript supply with demand.
Contributors: Guangyu Wang
... All data used in the paper except for the original .oir images due to the large size of the files. The pptx. contains all the data in the figures which can be opened with GraghPad Prism.
Contributors: aswin Sundarakrishnan
... The data associated with this manuscript is available here.
Contributors: Daniel Sorensen
... Raw data set for Vit 105 to titanium laser weld manuscript
Data for: Virtual Nondestructive Evaluation of Anisotropic Plates by Implementing Symmetry Informed Sequential Mapping of Anisotropic Green’s function (SISMAG)
Contributors: Sourav Banerjee, Sajan Shrestha
... No data should be used without permission from the corresponding the author. With permission, data can be used for only non-commercial purposes.
Laguesse_Prosapip1-Dependent Synaptic Adaptations In The Nucleus Accumbens Drive Alcohol Intake, Seeking, And Reward
Contributors: sophie laguesse
... Prosapip1-Dependent Synaptic Adaptations in the Nucleus Accumbens Drive Alcohol Intake, Seeking, and Reward Sophie Laguesse, Nadege Morisot6,7, Jung Hoon Shin6, Feng Liu8, Martin F. Adrover, Samuel A. Sakhai, Marcelo F. Lopez, Khanhky Phamluong, William C. Griffin III, Howard C. Becker, Kevin J. Bender, Veronica A. Alvarez, Dorit Ron DOI: http://dx.doi.org/10.1016/j.neuron.2017.08.037
Data for: Quantitative Analysis of Nucleic Acid-Metal Interactions and their Biological Implications
Contributors: Rachel Hellmann-Whitaker, Aleksandar Dimkovikj, Mallory Banton, Elizabeth Thorn, Lance McDanel, Katelin Arndt, Ana Royo Marco, Katelyn Unvert
... This data is a comprehensive quantitative analysis of nucleic acid-heavy metal interactions. Our abstract is as follows: RNA is known to interact with Mg2+ when assuming higher-ordered tertiary configurations. Structurally, when tRNA molecules interact with Mg2+, they consistently form a “L-shape” conformation each time they are synthesized. Therefore, if Mg2+ can induce tertiary structure formation, then binding to alternative cations could produce alternative tertiary configurations. By utilizing circular dichroism and mobility gel-shift assays it was observed that tRNA structure can be altered when in the presence of different divalent cationic species. Formation of these alternative structural configurations was further validated by aminoacylating these tRNA structural anomalies with their native enzyme, which resulted in markedly different degrees of activity. Thus, it was confirmed that structural changes do occur when tRNA forms complexes with different cations. To better understand these structural changes, quantitative cation binding to tRNA was determined through titrations as well as ICP-OES analysis, which indicated that the metal ions can bind to the tRNA structure in specific and non-specific ways. Lastly, it was observed through stopped-flow kinetics that tRNA can associate/dissociate from different cations to varying degrees, thus forming cation-specific complexes at unique rates.